| 1 |
Alkaline active xylanase (XynBYG) (promoter：P43) |
138 |
Bacillus subtilis WB700 |
Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
| 2 |
Alkaline active xylanase (XynBYG) (promoter：Pglvm) |
138 |
Bacillus subtilis WB700 |
Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
| 3 |
Alkaline polygalacturonate lyase (PGL) |
6 |
Bacillus subtilis WB600 |
Zhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 [PubMed] |
| 4 |
Alkaline protease (alk) |
35 |
Bacillus subtilis WB600 |
Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed] |
| 5 |
Alkaline protease (alk) |
86 |
Bacillus amyloliquefaciens 111018 |
Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed] |
| 6 |
Aminopeptidase (AP) |
20 |
Bacillus subtilis WB600 |
Guan C, Cui W, Cheng J, et al. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis[J]. New Biotechnology, 2016, 33(3): 372-379. [PubMed] |
| 7 |
Amylase (AmyA) |
18 |
Lactobacillus plantarum WCFS1 |
Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed] |
| 8 |
Bacillus protease novo type (BPN') |
11 |
Bacillus subtilis TEB1030 |
Degering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. [PubMed] |
| 9 |
Bacillus protease novo type (BPN') |
6 |
Bacillus licheniformis H402 |
Degering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. [PubMed] |
| 10 |
Bacillus protease novo type (BPN') |
3 |
Bacillus licheniformis MW3 |
Degering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. [PubMed] |
| 11 |
CotA-laccase |
7 |
Bacillus subtilis WB600 |
Guan Z B, Shui Y, Song C M, et al. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater[J]. Environmental Science and Pollution Research, 2015, 22(12): 9515-9523. [PubMed] |
| 12 |
Cutinase |
173 |
Bacillus subtilis TEB1030 |
Brockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. [PubMed] |
| 13 |
Cutinase |
64 |
Corynebacterium glutamicum |
Hemmerich J, Rohe P, Kleine B, et al. Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum[J]. Microbial Cell Factories, 2016, 15(1): 208. [PubMed] |
| 14 |
L-Asparaginase |
13 |
Bacillus subtilis WB600 |
Xuan Chen.Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600[D]. Jiangnan University, 2015. (in Chinese) |
| 15 |
Lipase (LipS) |
9 |
Bacillus subtilis WB800 |
Zhou Y, Xu G, Yang L, et al. Effect of signal Peptide Optimization on Lipase LipS secretion and expression in Bacillus subtilis system[J]. China Biotechnology, 2015, 35(9): 42-49. (in Chinese) |
| 16 |
Lipase A |
5 |
Bacillus subtilis WB600 |
LingLi Zeng.Effects of different signal peptides on the secretion of lipase A in Bacillus subtilis[D]. Jiangnan University, 2009. (in Chinese) |
| 17 |
Natto phytase |
15 |
Bacillus subtilis RIK1285 |
Tsuji S, Tanaka K, Takenaka S, et al. Enhanced secretion of natto phytase by Bacillus subtilis[J]. Bioscience Biotechnology and Biochemistry, 2015, 79(11): 1906-1914. [PubMed] |
| 18 |
Nattokinase |
81 |
Bacillus licheniformis △0F-3 |
Cai D, Wei X, Qiu Y, et al. High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase[J]. Journal of Applied Microbiology, 2016, 121(3): 704-712. [PubMed] |
| 19 |
Nuclease (NucA) |
78 |
Lactobacillus plantarum WCFS1 |
Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed] |
| 20 |
glucoamylase (TlGAMY) |
10 |
Yarrowia lipolytica JMY2101 |
Celinska E, Borkowska M, Bialas W, et al. Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags[J]. Applied Microbiology and Biotechnology, 2018, 102(12): 5221-5233. [PubMed] |
| 21 |
α-amylase (AmyE) |
31 |
Corynebacterium glutamicum |
Watanabe K, Tsuchida Y, Okibe N, et al. Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences[J]. Microbiology-Sgm, 2009, 155: 741-750. [PubMed] |
| 22 |
α-amylase (AmyS) |
16 |
Bacillus subtilis 1A751p |
Fu G, Liu J L, Li J S, et al. Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2018, 66(50): 13141-13151. [PubMed] |
| 23 |
α-amylase (AmyS) |
3 |
Bacillus subtilis RIK1285 |
Ya D, Su L, Li N, et al. Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection[J]. Microbial Cell Factories, 2019, 18: 69. [PubMed] |
| 24 |
α-amylase (AmyS) |
3 |
Bacillus subtilis WS11 |
Ya D, Su L, Li N, et al. Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection[J]. Microbial Cell Factories, 2019, 18: 69. [PubMed] |
| 25 |
α-amylase (AmyS) |
46 |
Bacillus subtilis 1A751 |
Jinlan L, Gang F, Huina D, et al. Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis[J]. Industrial Microbiology, 2017, 47(01): 17-23. (In Chinese) |
| 26 |
α-amylase (SoAMY) |
10 |
Yarrowia lipolytica JMY2101 |
Celinska E, Borkowska M, Bialas W, et al. Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags[J]. Applied Microbiology and Biotechnology, 2018, 102(12): 5221-5233. [PubMed] |