SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0001156

Accession Number SPSED0001156
Secreted Protein Cutinase
Uniprot Accession Number P00590
GenBank Accession Number AAA33335.1
Source Organism of the Secreted Protein Fusarium solani pisi
Nucleotide Sequence of the Secreted Protein Length = 693 nt
ATGAAATTCTTCGCTCTCACCACACTTCTCGCCGCCACGGCTTCGGCTCTGCCTACTTCTAACCCTGCCCAGGAGCTTGAGGCGCGCCAGCTTGGTAGAACAACTCGCGACGATCTGATCAACGGCAATAGCGCTTCCTGCGCCGATGTCATCTTCATTTATGCCCGAGGTTCAACAGAGACGGGCAACTTGGGAACTCTCGGTCCTAGCATTGCCTCCAACCTTGAGTCCGCCTTCGGCAAGGACGGTGTCTGGATTCAGGGCGTTGGCGGTGCCTACGCAGCCACTCTTGGAGACAATGCTCTCCCTCGCGGAACCTCTAGCGCCGCAATCAGGGAGATGCTCGGTCTCTTCCAGCAGGCCAACACCAAGTGCCCTGACGCGACTTTGATCGCCGGTGGCTACAGCCAGGGTGCTGCACTTGCAGCCGCCTCCATCGAGGACCTCGACTCGGCCATTCGTGACAAGATCGCCGGAACTGTTCTGTTCGGCTACACCAAGAACCTACAGAACCGTGGCCGAATCCCCAACTACCCTGCCGACAGGACCAAGGTCTTCTGCAATACAGGGGATCTCGTTTGTACTGGTAGCTTGATCGTTGCTGCACCTCACTTGGCTTATGGTCCTGATGCTCGTGGCCCTGCCCCTGAGTTCCTCATCGAGAAGGTTCGGGCTGTCCGTGGTTCTGCTTGA
Amino Acid Sequence of the Secreted Protein Length = 230 aa
MKFFALTTLLAATASALPTSNPAQELEARQLGRTTRDDLINGNSASCRDVIFIYARGSTETGNLGTLGPSIASNLESAFGKDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNTGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA
Expression Host Bacillus subtilis TEB1030

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide phoA
Signal Peptide Sequence Length = 30 aa
KKMSLFQNMKSKLLPIAAVSVLTAGIFAGA
Type Sec
Yield Signal peptides not tolerated in Bacillus subtilis when constitutively expressed as in-frame fusion to cutinase from Fusarium solani pisi -
Performance (Current Yield / Highest Yield) -
Charge 4
Charge (N-Region) 4
Hydrophobicity 0.76
Hydrophobicity Plot
Hydrophobicity (H-region) 2.23
Signal Peptide Original Protein Sequence KKMSLFQNMKSKLLPIAAVSVLTAGIFAGAAELQQTEKASAKKQDKAEIRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNNPKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVKTYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHATPAAYGAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGLFADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKAIIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK
Signal Peptide Original DNA Sequence ATGAAAAAAATGAGTTTGTTTCAAAATATGAAATCAAAACTTCTGCCAATCGCCGCTGTTTCTGTCCTTACAGCTGGAATCTTTGCCGGAGCTGAGCTTCAGCAAACAGAAAAGGCCAGCGCCAAAAAACAAGACAAAGCTGAGATCAGAAATGTCATTGTGATGATAGGCGACGGCATGGGGACGCCTTACATAAGAGCCTACCGTTCCATGAAAAATAACGGTGACACACCGAATAACCCGAAGTTAACAGAATTTGACCGGAACCTGACAGGCATGATGATGACGCATCCGGATGACCCTGACTATAATATTACAGATTCAGCAGCAGCCGGAACAGCATTAGCGACAGGCGTTAAGACATATAACAATGCAATTGGCGTCGATAAAAACGGAAAAAAAGTGAAATCTGTACTTGAAGAGGCCAAACAGCAAGGCAAGTCAACAGGGCTTGTCGCCACGTCTGAAATTAACCACGCCACTCCAGCCGCATATGGCGCCCACAATGAATCACGGAAAAACATGGACCAAATCGCCAACAGCTATATGGATGACAAGATAAAAGGCAAACATAAAATAGACGTGCTGCTCGGCGGCGGAAAATCTTATTTTAACCGCAAGGACAGAAACTTGACAAAGGAATTCAAACAAGCCGGCTACAGCTATGTGACAACTAAACAAGCATTGAAAAAAAATAAAGATCAGCAGGTGCTCGGGCTTTTCGCAGATGGAGGGCTTGCTAAAGCGCTCGACCGTGACAGTAAAACACCGTCTCTCAAAGACATGACGGTTTCAGCAATTGATCGCCTGAACCAAAATAAAAAAGGATTTTTCTTGATGGTCGAAGGGAGCCAGATTGACTGGGCGGCCCATGACAATGATACAGTAGGAGCCATGAGCGAGGTTAAAGATTTTGAACAGGCCTATAAAGCCGCGATTGAATTTGCGAAAAAAGACAAACATACACTTGTGATTGCAACTGCTGACCATACAACCGGCGGCTTTACCATTGGCGCAAACGGGGAAAAGAATTGGCACGCAGAACCGATTCTCTCCGCTAAGAAAACACCTGAATTCATGGCCAAAAAAATCAGTGAAGGCAAGCCGGTTAAAGATGTGCTCGCCCGCTATGCCAATCTGAAAGTCACATCTGAAGAAATCAAAAGCGTTGAAGCAGCTGCACAGGCTGACAAAAGCAAAGGGGCCTCCAAAGCCATCATCAAGATTTTTAATACCCGCTCCAACAGCGGATGGACGAGTACCGATCATACCGGCGAAGAAGTACCGGTATACGCGTACGGCCCCGGAAAAGAAAAATTCCGCGGATTGATTAACAATACGGACCAGGCAAACATCATATTTAAGATTTTAAAAACTGGAAAATAA
Reference Brockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. [PubMed]
Miscellaneous -