SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0002002
| Accession Number | SPSED0002002 |
| Secreted Protein | Aminopeptidase (AP) |
| Uniprot Accession Number | A0A023SGJ5 |
| GenBank Accession Number | AHX73749.1 |
| Source Organism of the Secreted Protein | Bacillus subtilis Zj016 |
| Nucleotide Sequence of the Secreted Protein | Length = 1368 nt ATGAAAAAGCTTTTGACTGTCATGACGATGGCTGTTTTAACTGCCGGCACACTGCTCTTGCCGGCACAGAGTGTCACCCCTGCCGCGCACGCTGTACAAATATCAAATAGCGAGCGCGAGCTTCTATTCAAAGCAAAACATGCGTACTCTACCATTTCTCAGCTAAGTGAAGCAATCGGCCCCAGAATAGCCGGAACTGCAGCTGAAAAAAAGAGTGCCCTATTGATCGCCTCATCAATGAGAAAATTAAAGCTTGATGTGAAGGTTCAACGATTCAACATTCCTGACCGGCTTGAGGGAACACTGTCTTCAGCAGGACGCGATATTCTTCTCCAAGCGGCGTCCGGCTCAGCTCCGACTGAGGAACAAGGACTGACGGCCCCGCTTTACAATGCGGGATTGGGCAATCAAAAGGGCTTTACCGCTGACGCCAAGGGCAAAATCGCCTTAATTTCCAGAGGAGACCTGACTTATTACGAGAAAGCCAAAAATGCCGAAGCCGCCGGAGCAAAAGCTGTCATCATTTATAACAACAAAGAAAGCCTCGTGCCTATGACGCCAAACCTGTCGGGAAATAAAGTCGGCATTCCGGTCGTCGGCATTAAGAAAGAAGACGGCGAAGCACTTACCCAGCAAAAAGAAGCCACCTTAAAACTAAAAGCATTCACAAACCAAACCTCCCAGAATATCATCGGAATCAAAAAACCAAAGAACATCAAACATCCAGACATTGTGTACGTGACGGCCCATTACGACAGTGTTCCTTTTTCGCCCGGAGCAAATGACAACGGCTCAGGTACCTCTGTTATGCTGGAGATGGCGCGTGTCTTAAAAAGCGTTCCATCTGATAAAGAAATCCGCTTTATCGCTTTCGGCGCCGAAGAGCTCGGCCTGCTCGGCTCCTCTCACTATGTAGATCATCTATCAGAAAAAGAGCTGAAACGAAGCGAAGTGAACTTCAACTTAGATATGGTAGGCACAAGCTGGGAAAAAGCGTCTGAGCTGTATGTCAACACATTGGACGGCCAATCTAACTATGTTTGGGAATCCAGCCGTACGGCTGCTGAAAAAATTGGGTTTGACAGCCTGTCTCTGACACAGGGAGGATTATCTGATCATGTGCCATTCCACGAAGCCGGCATTGATTCCGCCAACTTTATTTGGGGAGACCCGGAAACAGAAGAAGTTGAGCCGTGGTATCATACCCCTGAAGACTCGATCGAACATATCAGCAAAGAACGCCTCCAGCAAGCAGGCGATCTTGTGACAGCCGCTGTCTATGAGGCTGTGAAAAAAGAGAAGAAACCGAAAACCATTAAGAAACAAATGAAGGCGAAAGCATCTGACATTTTTGAAGATATCAAATAA |
| Amino Acid Sequence of the Secreted Protein | Length = 455 aa MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELLFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSMRKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGNQKGFTADAKGKIALISRGDLTYYEKAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIKHPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNFNLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGLSDHVPFHEAGIDSANFIWGDPETEEVEPWYHTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK |
| Expression Host | Bacillus subtilis WB600 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | eapD |
| Signal Peptide Sequence | Length = 24 aa MKKLLTVMTMAVLTAGTLLLPAQS |
| Type | Sec |
| Yield | 73.6 (U/ml) |
| Performance (Current Yield / Highest Yield) | 0.83 |
| Charge | 2 |
| Charge (N-Region) | 2 |
| Hydrophobicity | 1.06 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 1.88 |
| Signal Peptide Original Protein Sequence | MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSMRKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYEKAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIKHPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNFNLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWYHTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK |
| Signal Peptide Original DNA Sequence | ATGAAAAAGCTTTTGACTGTCATGACGATGGCTGTTTTAACTGCCGGCACACTGCTCTTGCCGGCACAGAGTGTCACCCCTGCCGCGCACGCTGTACAAATATCAAATAGCGAGCGCGAGCTTCCATTCAAAGCAAAACATGCGTACTCTACCATTTCTCAGCTAAGTGAAGCAATCGGCCCCAGAATAGCCGGAACTGCAGCTGAAAAAAAGAGTGCCCTATTGATCGCCTCATCAATGAGAAAATTAAAGCTTGATGTGAAGGTTCAACGATTCAACATTCCTGACCGGCTTGAGGGTACACTGTCTTCAGCAGGACGCGATATTCTTCTCCAAGCGGCGTCGGGCTCAGCTCCGACTGAGGAACAAGGGCTGACGGCCCCGCTTTACAATGCGGGATTGGGCTATCAAAAGGACTTTACCGCTGACGCCAAGGGCAAAATCGCCTTGATTTCCAGAGGAGACCTGACTTATTACGAGAAAGCCAAAAATGCCGAAGCCGCCGGAGCGAAAGCTGTCATCATTTATAACAACAAAGAAAGCCTCGTGCCTATGACGCCAAACCTGTCGGGAAATAAAGTCGGCATTCCGGTCGTCGGCATTAAGAAAGAAGACGGCGAAGCACTTACCCAGCAAAAAGAAGCCACCTTAAAACTAAAAGCATTCACAAACCAAACCTCCCAGAATATCATCGGAATCAAAAAACCAAAGAACATCAAACATCCAGACATTGTGTACGTGACCGCCCATTACGACAGTGTTCCTTTTTCGCCCGGAGCAAATGACAACGGCTCAGGTACCTCTGTTATGCTGGAGATGGCGCGTGTCTTAAAAAGCGTTCCATCTGATAAAGAAATCCGCTTTATCGCTTTCGGCGCCGAAGAGCTCGGCCTGCTCGGCTCCTCTCACTATGTAGATCATCTATCGGAAAAAGAGCTGAAACGAAGCGAAGTGAACTTCAACTTAGATATGGTAGGCACAAGCTGGGAAAAAGCGTCTGAGCTGTATGTCAACACATTGGATGGCCAATCTAACTATGTTTGGGAATCCAGCCGTACGGCTGCTGAAAAAATTGGGTTTGACAGCCTGTCTCTGACACAGGGAGGATCATCTGATCATGTGCCATTCCACGAAGCCGGCATTGATTCCGCTAACTTTATTTGGGGAGACCCGGAAACAGAAGAAGTTGAGCCGTGGTATCATACCCCTGAAGACTCGATCGAACATATCAGCAAAGAACGCCTCCAGCAAGCAGGCGATCTTGTGACAGCCGCTGTCTATGAGGCTGTGAAAAAAGAGAAGAAACCGAAAACCATTAAGAAACAAATGAAGGCGAAAGCATCTGACATTTTTGAAGATATCAAATAA |
| Reference | Guan C, Cui W, Cheng J, et al. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis[J]. New Biotechnology, 2016, 33(3): 372-379. [PubMed] |
| Miscellaneous | - |