SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0002018

Accession Number SPSED0002018
Secreted Protein Aminopeptidase (AP)
Uniprot Accession Number A0A023SGJ5
GenBank Accession Number AHX73749.1
Source Organism of the Secreted Protein Bacillus subtilis Zj016
Nucleotide Sequence of the Secreted Protein Length = 1368 nt
ATGAAAAAGCTTTTGACTGTCATGACGATGGCTGTTTTAACTGCCGGCACACTGCTCTTGCCGGCACAGAGTGTCACCCCTGCCGCGCACGCTGTACAAATATCAAATAGCGAGCGCGAGCTTCTATTCAAAGCAAAACATGCGTACTCTACCATTTCTCAGCTAAGTGAAGCAATCGGCCCCAGAATAGCCGGAACTGCAGCTGAAAAAAAGAGTGCCCTATTGATCGCCTCATCAATGAGAAAATTAAAGCTTGATGTGAAGGTTCAACGATTCAACATTCCTGACCGGCTTGAGGGAACACTGTCTTCAGCAGGACGCGATATTCTTCTCCAAGCGGCGTCCGGCTCAGCTCCGACTGAGGAACAAGGACTGACGGCCCCGCTTTACAATGCGGGATTGGGCAATCAAAAGGGCTTTACCGCTGACGCCAAGGGCAAAATCGCCTTAATTTCCAGAGGAGACCTGACTTATTACGAGAAAGCCAAAAATGCCGAAGCCGCCGGAGCAAAAGCTGTCATCATTTATAACAACAAAGAAAGCCTCGTGCCTATGACGCCAAACCTGTCGGGAAATAAAGTCGGCATTCCGGTCGTCGGCATTAAGAAAGAAGACGGCGAAGCACTTACCCAGCAAAAAGAAGCCACCTTAAAACTAAAAGCATTCACAAACCAAACCTCCCAGAATATCATCGGAATCAAAAAACCAAAGAACATCAAACATCCAGACATTGTGTACGTGACGGCCCATTACGACAGTGTTCCTTTTTCGCCCGGAGCAAATGACAACGGCTCAGGTACCTCTGTTATGCTGGAGATGGCGCGTGTCTTAAAAAGCGTTCCATCTGATAAAGAAATCCGCTTTATCGCTTTCGGCGCCGAAGAGCTCGGCCTGCTCGGCTCCTCTCACTATGTAGATCATCTATCAGAAAAAGAGCTGAAACGAAGCGAAGTGAACTTCAACTTAGATATGGTAGGCACAAGCTGGGAAAAAGCGTCTGAGCTGTATGTCAACACATTGGACGGCCAATCTAACTATGTTTGGGAATCCAGCCGTACGGCTGCTGAAAAAATTGGGTTTGACAGCCTGTCTCTGACACAGGGAGGATTATCTGATCATGTGCCATTCCACGAAGCCGGCATTGATTCCGCCAACTTTATTTGGGGAGACCCGGAAACAGAAGAAGTTGAGCCGTGGTATCATACCCCTGAAGACTCGATCGAACATATCAGCAAAGAACGCCTCCAGCAAGCAGGCGATCTTGTGACAGCCGCTGTCTATGAGGCTGTGAAAAAAGAGAAGAAACCGAAAACCATTAAGAAACAAATGAAGGCGAAAGCATCTGACATTTTTGAAGATATCAAATAA
Amino Acid Sequence of the Secreted Protein Length = 455 aa
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELLFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSMRKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGNQKGFTADAKGKIALISRGDLTYYEKAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIKHPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNFNLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGLSDHVPFHEAGIDSANFIWGDPETEEVEPWYHTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide penP
Signal Peptide Sequence Length = 36 aa
MKLKTKASIKFGICVGLLCLSITGFTPFFNSTHAEA
Type Sec
Yield 2.6 (U/ml)
Performance (Current Yield / Highest Yield) 0.03
Charge 3
Charge (N-Region) 4
Hydrophobicity 0.63
Hydrophobicity Plot
Hydrophobicity (H-region) 1.88
Signal Peptide Original Protein Sequence MKLKTKASIKFGICVGLLCLSITGFTPFFNSTHAEAKSIEDTNMASCITNKKFVQLEKKFDARLGVYAIDIGSNKTIAYRPNERFAYASTYKVLAAAAVLKKNSIEKLNEVIHYSKDDLVTYSPITEKHLDTGMSLKEISEAAIRYSDNTAGNILLQQLGGPKGFEKSLKQIGDHVTKAKRFETDLNSAIPGDIRDTSTAKALATDLKAFTLDNTLTTDKRMILTDWMRGNATGDELIRAGAPIGWEVGDKSGAGSYGTRNDIAIVWPPNRAPIVVAILSNRFTKDANYDNALIAEAAKVVLNDLK
Signal Peptide Original DNA Sequence ATGAAGTTGAAAACTAAAGCGTCAATAAAATTCGGAATATGTGTTGGGCTTTTATGTTTAAGCATTACTGGTTTCACACCTTTTTTCAACTCAACACATGCCGAAGCAAAATCCATAGAAGACACAAACATGGCAAGCTGTATAACGAATAAAAAATTTGTTCAACTTGAGAAAAAATTTGATGCCAGGCTTGGTGTTTATGCAATTGATATAGGTTCAAACAAGACAATCGCCTATCGGCCGAATGAACGATTTGCTTACGCATCAACCTATAAAGTTCTAGCCGCAGCTGCTGTCCTGAAGAAAAACTCAATTGAAAAACTTAATGAAGTCATCCACTACAGCAAGGACGATCTTGTTACATATTCTCCAATCACAGAGAAACACCTAGATACAGGCATGAGCCTAAAAGAAATCTCGGAAGCGGCTATCCGTTACAGCGATAATACCGCTGGGAACATCTTGTTGCAACAGCTTGGCGGCCCTAAAGGTTTTGAAAAGTCACTGAAACAGATTGGGGATCATGTAACGAAAGCTAAACGATTCGAGACGGATCTAAACTCTGCGATTCCTGGTGATATTCGTGACACCAGCACCGCAAAAGCACTGGCAACCGATCTTAAAGCCTTCACTTTAGATAACACTCTCACGACTGACAAACGCATGATCTTAACCGATTGGATGCGAGGCAATGCTACTGGTGACGAACTGATTCGGGCAGGCGCTCCAATAGGATGGGAAGTCGGCGATAAATCCGGAGCCGGAAGTTACGGGACACGAAACGACATTGCTATCGTTTGGCCGCCAAACAGAGCTCCTATTGTTGTGGCAATTCTGTCAAACCGCTTTACGAAAGATGCTAATTATGATAATGCTCTTATCGCTGAGGCTGCAAAAGTCGTCCTTAACGATCTCAAATAA
Reference Guan C, Cui W, Cheng J, et al. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis[J]. New Biotechnology, 2016, 33(3): 372-379. [PubMed]
Miscellaneous -