SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0003006

Accession Number SPSED0003006
Secreted Protein Nuclease (NucA)
Uniprot Accession Number P00644
GenBank Accession Number CAA24594.1
Source Organism of the Secreted Protein Staphylococcus aureus
Nucleotide Sequence of the Secreted Protein Length = 696 nt
GTGTTAGTTATGACAGAATACTTATTAAGTGCTGGCATATGTATGGCAATTGTTTCAATATTACTTATAGGGATGGCTATCAGTAATGTTTCGAAAGGGCAATACGCAAAGAGGTTTTTCTTTTTCGCTACTAGTTGCTTAGTGTTAACTTTAGTTGTAGTTTCAAGTCTAAGTAGCTCAGCAAATGCATCACAAACAGATAACGGCGTAAATAGAAGTGGTTCTGAAGATCCAACAGTATATAGTGCAACTTCAACTAAAAAATTACATAAAGAACCTGCGACTTTAATTAAAGCGATTGATGGTGATACGGTTAAATTAATGTACAAAGGTCAACCAATGACATTCAGACTATTATTGGTTGATACACCTGAAACAAAGCATCCTAAAAAAGGTGTAGAGAAATATGGTCCTGAAGCAAGTGCATTTACGAAAAAAATGGTAGAAAATGCAAAGAAAATTGAAGTCGAGTTTGACAAAGGTCAAAGAACTGATAAATATGGACGTGGCTTAGCGTATATTTATGCTGATGGAAAAATGGTAAACGAAGCTTTAGTTCGTCAAGGCTTGGCTAAAGTTGCTTATGTTTACAAACCTAACAATACACATGAACAACATTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAA
Amino Acid Sequence of the Secreted Protein Length = 231 aa
MLVMTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ
Expression Host Lactobacillus plantarum WCFS1

Source Organism of the Signal Peptide Lactobacillus plantarum WCFS1
Source Gene of the Signal Peptide tag1
Signal Peptide Sequence Length = 27 aa
MKEVRFWGLLLGLFVCLGAVIPLVSKA
Type Sec
Yield 16.64 (U/ml)
Performance (Current Yield / Highest Yield) 0.46
Charge 2
Charge (N-Region) 1
Hydrophobicity 1.39
Hydrophobicity Plot
Hydrophobicity (H-region) 2.43
Signal Peptide Original Protein Sequence MKEVRFWGLLLGLFVCLGAVIPLVSKADVLDTAPAGLNLEGLFEPGKFDRNSATMQTVLKNGHSQHVIELTNDDNQVGAMWSTPVNRFNMRVDNQVSMWVNFGDKAVSEGLAVVLQNDPHKMSAISRYGKKSIIGENLGVYGTDTYTRRTAPATIAKTAIQKSWALELDTKVNDGGFWGLYPRGNSFDEKPKKMHMAASYPASPDTYTQRGGLTSSYRFEQNHGSIADKLPLADGNWHHLVLKWVASKQIMSYTLDQGPTRMTTIDTTVFGKDIRNVTWGLTSTATTANARTLVVFDRLPQPIEIESDLNVTNLTQERTVHEGDWVHGGDELEYEYVLSYLNGDNEWQQVKASLPLPEQVHFSHAKIRYADGTDEEINLPADDNESSVARLQHDLQRTLSMKNPHAYITLIGEAAKVDRDTFVDDDMSYFEGHEMKTATATPHFTITTNKTVNLTIEPLTQVKKGEPVIIKGQIKLNDGLDFDNDGMRIEYRVNGEAGEDYQLSMDDPVGYVEFEVDAEELQIGENQITLRAHSKDNAYSEPVTVTVTVIGGELQLKKVSTESVFAPIKLTGTAQVSRPEGLEVVVNDDTGSKAAWRLEATATPLVTNQGRTLAGNLYYQDGAKLTMLGKDAVEIARQKTAPGIDYDVTAHWSSQQGLVLKTTPAAVPGSYQGTITWHLSTVPEE
Signal Peptide Original DNA Sequence GTGAAAGAAGTAAGGTTTTGGGGACTCTTGTTAGGACTGTTTGTCTGTTTAGGGGCAGTGATACCGTTAGTTAGTAAGGCTGACGTACTTGATACGGCACCCGCTGGACTGAATCTGGAAGGCTTATTTGAACCGGGAAAGTTTGACCGTAATTCGGCCACAATGCAGACTGTTTTAAAGAATGGCCATTCGCAGCATGTTATCGAATTAACAAATGACGATAATCAAGTTGGGGCAATGTGGTCAACCCCTGTTAATCGCTTTAACATGCGGGTCGATAACCAAGTCTCAATGTGGGTGAACTTTGGGGACAAAGCCGTGAGTGAAGGACTGGCCGTTGTTTTACAGAATGATCCCCACAAAATGAGTGCGATTAGTCGGTATGGAAAGAAAAGCATTATTGGTGAGAACTTGGGTGTTTATGGGACGGATACCTATACACGGCGAACGGCGCCGGCCACGATTGCTAAGACGGCGATTCAAAAAAGTTGGGCGTTGGAGCTGGATACCAAAGTGAATGACGGTGGTTTTTGGGGATTGTATCCCCGGGGGAATTCTTTCGATGAAAAGCCTAAAAAAATGCATATGGCCGCGAGCTACCCTGCAAGTCCGGATACTTATACGCAACGGGGTGGTTTAACGTCCTCTTATCGTTTTGAACAGAACCATGGGAGTATCGCTGATAAATTACCACTCGCGGACGGTAACTGGCACCATCTTGTATTGAAGTGGGTCGCCAGTAAACAAATCATGAGTTATACCCTGGATCAGGGACCAACACGAATGACCACAATTGATACAACAGTCTTTGGAAAGGATATCCGGAATGTTACTTGGGGATTAACTAGTACGGCGACAACCGCTAATGCCAGAACACTGGTCGTCTTTGACCGGTTGCCACAACCGATTGAAATCGAGTCAGATTTAAACGTAACCAATCTGACTCAAGAGCGAACTGTCCATGAAGGAGACTGGGTTCATGGTGGGGATGAGTTGGAATATGAGTACGTTCTGAGCTATCTCAATGGTGACAATGAATGGCAACAGGTTAAGGCCAGCCTACCATTACCCGAACAAGTTCACTTTAGTCACGCGAAGATCCGCTATGCGGATGGTACCGATGAAGAGATCAATTTACCAGCGGACGATAACGAATCGTCGGTGGCCCGCTTACAACACGATTTGCAGCGGACACTATCAATGAAAAATCCGCACGCTTATATTACGCTGATTGGTGAGGCTGCCAAGGTCGATCGGGATACTTTTGTTGATGATGACATGAGTTACTTTGAGGGTCACGAGATGAAGACCGCAACGGCCACACCGCACTTCACCATTACGACCAATAAGACCGTTAACCTGACGATTGAACCGTTGACACAAGTGAAGAAAGGCGAGCCCGTCATCATTAAGGGCCAAATTAAGCTAAATGATGGGTTAGACTTTGACAATGATGGCATGCGTATCGAGTACCGGGTCAATGGTGAAGCGGGAGAAGACTATCAGTTGAGCATGGACGATCCGGTTGGTTATGTCGAATTTGAAGTGGATGCCGAAGAACTACAAATCGGTGAAAATCAGATTACGTTACGGGCTCATAGCAAAGATAACGCCTACTCAGAACCAGTGACAGTAACGGTCACGGTCATTGGTGGGGAGTTACAGCTGAAAAAAGTCAGTACTGAAAGTGTGTTTGCACCAATCAAGTTGACGGGAACGGCTCAAGTCAGTCGGCCCGAGGGACTTGAAGTAGTTGTCAATGATGATACGGGCTCGAAAGCCGCTTGGCGCTTAGAGGCGACCGCGACCCCTCTCGTGACTAATCAGGGCCGCACACTAGCGGGAAACTTGTATTATCAGGATGGTGCCAAGCTGACAATGTTGGGAAAGGATGCCGTGGAAATCGCGCGCCAAAAGACGGCCCCGGGGATCGACTATGACGTGACCGCCCACTGGTCATCGCAACAAGGTTTGGTGCTCAAAACAACGCCCGCCGCTGTTCCTGGAAGTTATCAGGGTACGATCACGTGGCACTTAAGTACCGTACCGGAAGAGTGA
Reference Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed]
Miscellaneous -