SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0003011
| Accession Number | SPSED0003011 |
| Secreted Protein | Nuclease (NucA) |
| Uniprot Accession Number | P00644 |
| GenBank Accession Number | CAA24594.1 |
| Source Organism of the Secreted Protein | Staphylococcus aureus |
| Nucleotide Sequence of the Secreted Protein | Length = 696 nt GTGTTAGTTATGACAGAATACTTATTAAGTGCTGGCATATGTATGGCAATTGTTTCAATATTACTTATAGGGATGGCTATCAGTAATGTTTCGAAAGGGCAATACGCAAAGAGGTTTTTCTTTTTCGCTACTAGTTGCTTAGTGTTAACTTTAGTTGTAGTTTCAAGTCTAAGTAGCTCAGCAAATGCATCACAAACAGATAACGGCGTAAATAGAAGTGGTTCTGAAGATCCAACAGTATATAGTGCAACTTCAACTAAAAAATTACATAAAGAACCTGCGACTTTAATTAAAGCGATTGATGGTGATACGGTTAAATTAATGTACAAAGGTCAACCAATGACATTCAGACTATTATTGGTTGATACACCTGAAACAAAGCATCCTAAAAAAGGTGTAGAGAAATATGGTCCTGAAGCAAGTGCATTTACGAAAAAAATGGTAGAAAATGCAAAGAAAATTGAAGTCGAGTTTGACAAAGGTCAAAGAACTGATAAATATGGACGTGGCTTAGCGTATATTTATGCTGATGGAAAAATGGTAAACGAAGCTTTAGTTCGTCAAGGCTTGGCTAAAGTTGCTTATGTTTACAAACCTAACAATACACATGAACAACATTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAA |
| Amino Acid Sequence of the Secreted Protein | Length = 231 aa MLVMTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ |
| Expression Host | Lactobacillus plantarum WCFS1 |
| Source Organism of the Signal Peptide | Lactobacillus plantarum WCFS1 |
| Source Gene of the Signal Peptide | fhuG |
| Signal Peptide Sequence | Length = 28 aa MQKRLRLSLGMLLAVVASLLMMGQVASA |
| Type | Sec |
| Yield | 9.69 (U/ml) |
| Performance (Current Yield / Highest Yield) | 0.27 |
| Charge | 3 |
| Charge (N-Region) | 3 |
| Hydrophobicity | 1.1 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 2.41 |
| Signal Peptide Original Protein Sequence | MQKRLRLSLGMLLAVVASLLMMGQVASADQGVGFEITPVQSSSQVDKRLSYFDLKLKPRQTQTVAVKIHNTASEPITINIGIAKATTNINGVVEYKHTNNRSANLPYDISKLVTTDEPQVKLAKGQVKTVKFQIKMPAQSYQGILAGGITFLKKATATDTKKAVAVNNQYAYTEAVILHGSKDLTTNQLTMNKITVKQLNGRNAINFPLVNHTAAYLNKVQTNIKIYHRGGSQVVYHQKLANGQMAPNSIYKLPLQTGEKALAAGKYTAVVKVTSKKQHWQFKQNFVITKGQADTLNKTAVLKQGPNWWLYIGIGLLLLLLIILVIWYIWRKQKKIKSLEKQLAEKNRQDSES |
| Signal Peptide Original DNA Sequence | GTGCAGAAACGATTGCGATTATCTTTGGGAATGTTATTGGCGGTTGTCGCTAGTCTATTAATGATGGGACAAGTAGCCTCTGCTGATCAGGGCGTTGGTTTTGAAATCACGCCAGTCCAGTCTAGCAGTCAGGTCGATAAGCGTCTCTCTTACTTTGATTTGAAGTTAAAACCGCGACAAACACAAACCGTTGCGGTCAAAATTCATAATACGGCTAGTGAGCCCATTACGATCAATATAGGTATCGCTAAGGCCACGACAAATATTAATGGGGTTGTGGAATACAAACATACGAATAATCGCAGTGCGAATTTGCCGTATGATATTAGCAAATTAGTGACAACTGATGAACCACAAGTCAAGCTGGCTAAGGGGCAAGTCAAGACGGTCAAGTTTCAAATTAAGATGCCGGCCCAAAGTTATCAAGGCATTTTAGCAGGTGGGATAACTTTCCTGAAAAAGGCCACGGCAACTGATACTAAAAAGGCCGTGGCAGTCAATAATCAATACGCTTATACTGAAGCTGTAATTTTGCATGGTAGTAAAGACCTAACGACCAATCAGCTGACGATGAATAAGATTACGGTCAAACAGCTCAATGGGCGCAATGCTATTAATTTTCCGTTAGTCAACCATACGGCGGCTTACTTAAATAAAGTACAAACTAACATCAAGATTTATCATCGGGGTGGCAGTCAAGTTGTCTACCATCAAAAGTTAGCGAACGGCCAGATGGCACCTAATTCAATCTATAAATTGCCATTGCAGACAGGAGAAAAAGCGCTAGCAGCTGGTAAATACACGGCCGTCGTGAAAGTTACTTCCAAAAAGCAACACTGGCAATTTAAGCAGAATTTTGTGATTACAAAGGGCCAGGCGGATACCTTGAATAAGACAGCGGTTTTGAAGCAAGGGCCTAACTGGTGGTTGTATATCGGGATTGGGTTATTACTGTTATTATTGATTATCCTAGTGATCTGGTATATTTGGCGCAAACAGAAGAAAATTAAATCGTTAGAGAAACAGTTAGCTGAAAAGAACCGTCAAGATAGTGAATCATAA |
| Reference | Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed] |
| Miscellaneous | - |