Detail Information for SPSED0004009

Accession Number SPSED0004009
Secreted Protein Amylase (AmyA)
Uniprot Accession Number A4ULJ3
GenBank Accession Number ABO77965.1
Source Organism of the Secreted Protein Lactobacillus amylovorus NRRL B-4549
Nucleotide Sequence of the Secreted Protein Length = 1434 nt
ATGAAAAAAAAGAAAAGTTTCTGGCTTGTTTCTTTTTTAGTTATAGTAGCTAGTGTTTTCTTTATATCTTTTGGATTTAGCAATCATTCTAAACAAGTTGCTCAAGCGGCTAGTGATACGACATCAACTGATCACTCAAGCAATGATACAGCTGATTCTGTTAGCGACGGTGTTATTTTGCATGCATGGTGCTGGTCGTTCAACACGATTAAAAACAACTTGAAACAGATTCATGACGCCGGCTACACAGCGGTTCAAACTTCACCTGTTAATGAAGTTAAAGTTGGAAATAGCGGGTCTAAGTCATTAAATAACTGGTATTGGCTATATCAGCCAACTAAATATAGTATTGGTAACTATTATTTAGGAACGGAAGCTGAATTTAAGTCAATGTGCGCTGCTGCTAAAGAATATAATATCAGGATCATTGTCGATGCAACTCTGAATGATACAACAAGTGATTATAGTGCAATTTCGGATGAAATTAAAAGTATTCCAAATTGGACACATGGTAACAAACAAATTTCGAATTGGAGTGATCGTGAAGATGTTACTCAAAATTCGTTGTTAGGTTTATATGATTGGAATACTCAAAATTCTCAAGTTCAGACGTATTTGAAGAATTATTTGGAACGCTTGATTTCTGACGGAGCTTCAGGCTTTCGTTATGATGCAGCTAAGCATATTGAACTTCCAAGTCAATATGATGGCAGCTATGGCAGCAATTTCTGGCCAAATATTACTGATAATGGGTCTGAATTTCAGTATGGTGAAGTTTTGCAGGACTCGATTTCAAAAGAATCAGATTATGCTAATTACATGAGTGTTACAGCTTCAAATTACGGCAATACGATTCGCAATGCGTTAAAGAATCGTGATTTTACCGCAAGTACTTTGCAGAATTTCAACATCAGTGTTCCAGCTTCTAAATTAGTAACTTGGGTCGAATCGCATGATAATTATGCTAACGATGATCAAGTTTCGACTTGGATGAATAATAGTGATATTAAATTAGGCTGGGCTGTTGTTGCTTCGCGTTCTGGTAGTGTTCCGCTGTTCTTTGACCGTCCAGTTGATGGTGGTAATGGTACTCGGTTCCCTGGCAGTTCAGAAATTGGTGATGCTGGCAGCAGTTTGTATTATGATAAAGCAGTTGTAGCTGTTAATAAATTCCATAATGCAATGGCTGGTCAATCTGAATATATTTCTAATCCAAATGGCAATACCAAGATTTTTGAAAATGAACGTGGCAGCAAAGGGGTTGTTTTTGCAAACGCTTCCGACAGTTCATATAGTTTGAATGTTAAAACTAGTTTAGCTGATGGGACTTATGAAAACAAGGCTGGTTCAGATGAATTTACCGTTAAAAATGGTTATTTAACCGGTACAATTCAAGGACGTGAAGTTGTTGTTCTTTACGGGGATCCAACAAGC
Amino Acid Sequence of the Secreted Protein Length = 478 aa
MKKKKSFWLVSFLVIVASVFFISFGFSNHSKQVAQAASDTTSTDHSSNDTADSVSDGVILHAWCWSFNTIKNNLKQIHDAGYTAVQTSPVNEVKVGNSGSKSLNNWYWLYQPTKYSIGNYYLGTEAEFKSMCAAAKEYNIRIIVDATLNDTTSDYSAISDEIKSIPNWTHGNKQISNWSDREDVTQNSLLGLYDWNTQNSQVQTYLKNYLERLISDGASGFRYDAAKHIELPSQYDGSYGSNFWPNITDNGSEFQYGEVLQDSISKESDYANYMSVTASNYGNTIRNALKNRDFTASTLQNFNISVPASKLVTWVESHDNYANDDQVSTWMNNSDIKLGWAVVASRSGSVPLFFDRPVDGGNGTRFPGSSEIGDAGSSLYYDKAVVAVNKFHNAMAGQSEYISNPNGNTKIFENERGSKGVVFANASDSSYSLNVKTSLADGTYENKAGSDEFTVKNGYLTGTIQGREVVVLYGDPTS
Expression Host Lactobacillus plantarum WCFS1

Source Organism of the Signal Peptide Lactobacillus plantarum WCFS1
Source Gene of the Signal Peptide amd
Signal Peptide Sequence Length = 39 aa
MKKFNFKTMLLLVLASCVFGVVVNVTTSLGPQTAITAQA
Type Sec
Yield 0.57 (10^2mU/mL)
Performance (Current Yield / Highest Yield) 0.17
Charge 3
Charge (N-Region) 3
Hydrophobicity 0.96
Hydrophobicity Plot
Hydrophobicity (H-region) 2.34
Signal Peptide Original Protein Sequence MKKFNFKTMLLLVLASCVFGVVVNVTTSLGPQTAITAQASKKLSQAQQIAKINAKLSKANRAAKNWIAYRESGYSYTARNGRCYGRYQLLRSYLHGNLSPVNQEKRANTYVASRYGSWVKAKRFWQHHHWY
Signal Peptide Original DNA Sequence ATGAAAAAATTTAACTTTAAAACCATGTTGCTATTAGTTTTGGCTAGTTGTGTCTTCGGGGTCGTCGTTAACGTGACTACTAGTCTTGGACCACAAACCGCAATCACCGCCCAGGCCTCCAAGAAGCTCAGCCAAGCCCAGCAAATTGCTAAGATCAACGCCAAGCTTTCGAAGGCTAACCGCGCTGCCAAAAATTGGATCGCCTATCGTGAATCTGGATACAGTTATACGGCTCGTAATGGCCGCTGCTACGGTCGCTATCAATTGTTGCGCTCATACTTACACGGCAACTTGTCACCCGTCAATCAGGAAAAACGTGCTAATACCTACGTCGCCTCGCGCTACGGTTCCTGGGTCAAGGCCAAACGGTTCTGGCAACATCATCACTGGTACTAA
Reference Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed]
Miscellaneous Cell fractions[102mU/mL] 7.2 Standard deviation 0.6 Secretion efficiency(%) 7