Accession Number |
SPSED0004012 |
Secreted Protein |
Amylase (AmyA) |
Uniprot Accession Number |
A4ULJ3 |
GenBank Accession Number |
ABO77965.1 |
Source Organism of the Secreted Protein |
Lactobacillus amylovorus NRRL B-4549 |
Nucleotide Sequence of the Secreted Protein |
Length = 1434 ntATGAAAAAAAAGAAAAGTTTCTGGCTTGTTTCTTTTTTAGTTATAGTAGCTAGTGTTTTCTTTATATCTTTTGGATTTAGCAATCATTCTAAACAAGTTGCTCAAGCGGCTAGTGATACGACATCAACTGATCACTCAAGCAATGATACAGCTGATTCTGTTAGCGACGGTGTTATTTTGCATGCATGGTGCTGGTCGTTCAACACGATTAAAAACAACTTGAAACAGATTCATGACGCCGGCTACACAGCGGTTCAAACTTCACCTGTTAATGAAGTTAAAGTTGGAAATAGCGGGTCTAAGTCATTAAATAACTGGTATTGGCTATATCAGCCAACTAAATATAGTATTGGTAACTATTATTTAGGAACGGAAGCTGAATTTAAGTCAATGTGCGCTGCTGCTAAAGAATATAATATCAGGATCATTGTCGATGCAACTCTGAATGATACAACAAGTGATTATAGTGCAATTTCGGATGAAATTAAAAGTATTCCAAATTGGACACATGGTAACAAACAAATTTCGAATTGGAGTGATCGTGAAGATGTTACTCAAAATTCGTTGTTAGGTTTATATGATTGGAATACTCAAAATTCTCAAGTTCAGACGTATTTGAAGAATTATTTGGAACGCTTGATTTCTGACGGAGCTTCAGGCTTTCGTTATGATGCAGCTAAGCATATTGAACTTCCAAGTCAATATGATGGCAGCTATGGCAGCAATTTCTGGCCAAATATTACTGATAATGGGTCTGAATTTCAGTATGGTGAAGTTTTGCAGGACTCGATTTCAAAAGAATCAGATTATGCTAATTACATGAGTGTTACAGCTTCAAATTACGGCAATACGATTCGCAATGCGTTAAAGAATCGTGATTTTACCGCAAGTACTTTGCAGAATTTCAACATCAGTGTTCCAGCTTCTAAATTAGTAACTTGGGTCGAATCGCATGATAATTATGCTAACGATGATCAAGTTTCGACTTGGATGAATAATAGTGATATTAAATTAGGCTGGGCTGTTGTTGCTTCGCGTTCTGGTAGTGTTCCGCTGTTCTTTGACCGTCCAGTTGATGGTGGTAATGGTACTCGGTTCCCTGGCAGTTCAGAAATTGGTGATGCTGGCAGCAGTTTGTATTATGATAAAGCAGTTGTAGCTGTTAATAAATTCCATAATGCAATGGCTGGTCAATCTGAATATATTTCTAATCCAAATGGCAATACCAAGATTTTTGAAAATGAACGTGGCAGCAAAGGGGTTGTTTTTGCAAACGCTTCCGACAGTTCATATAGTTTGAATGTTAAAACTAGTTTAGCTGATGGGACTTATGAAAACAAGGCTGGTTCAGATGAATTTACCGTTAAAAATGGTTATTTAACCGGTACAATTCAAGGACGTGAAGTTGTTGTTCTTTACGGGGATCCAACAAGC |
Amino Acid Sequence of the Secreted Protein |
Length = 478 aaMKKKKSFWLVSFLVIVASVFFISFGFSNHSKQVAQAASDTTSTDHSSNDTADSVSDGVILHAWCWSFNTIKNNLKQIHDAGYTAVQTSPVNEVKVGNSGSKSLNNWYWLYQPTKYSIGNYYLGTEAEFKSMCAAAKEYNIRIIVDATLNDTTSDYSAISDEIKSIPNWTHGNKQISNWSDREDVTQNSLLGLYDWNTQNSQVQTYLKNYLERLISDGASGFRYDAAKHIELPSQYDGSYGSNFWPNITDNGSEFQYGEVLQDSISKESDYANYMSVTASNYGNTIRNALKNRDFTASTLQNFNISVPASKLVTWVESHDNYANDDQVSTWMNNSDIKLGWAVVASRSGSVPLFFDRPVDGGNGTRFPGSSEIGDAGSSLYYDKAVVAVNKFHNAMAGQSEYISNPNGNTKIFENERGSKGVVFANASDSSYSLNVKTSLADGTYENKAGSDEFTVKNGYLTGTIQGREVVVLYGDPTS |
Expression Host |
Lactobacillus plantarum WCFS1 |
|
Source Organism of the Signal Peptide |
Lactobacillus plantarum WCFS1 |
Source Gene of the Signal Peptide |
hpk9 |
Signal Peptide Sequence |
Length = 30 aaMKKLMIAVTGLAMVATLGAAVPVTGITANA |
Type |
Sec |
Yield |
0.43 (10^2mU/mL) |
Performance (Current Yield / Highest Yield) |
0.13 |
Charge |
2 |
Charge (N-Region) |
2 |
Hydrophobicity |
1.29 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
1.85 |
Signal Peptide Original Protein Sequence |
MKKLMIAVTGLAMVATLGAAVPVTGITANAATKKTTATKNVAPSLKVNAIYNNTTQITGTATKGAKITVKSTANAKKNLGTATASKTTGKYTVKLAKTLKAQSNVYVYATNPTTKAYFYRVIRVQTAKTTASSTKKTTNKTTTTKKVATKSTAATSSSKKTTKSFKVNAPAGTWKSNTTNKYSQILTFSAKNGFNQTLYKNGKQVKKLVSYASYSMTAKTPTFWKVSYKSGSTTKTLYLRYTATNKFTVVNAKNQIVKVKVGNAPAAVWTFTKK |
Signal Peptide Original DNA Sequence |
ATGAAGAAATTGATGATTGCTGTAACTGGTTTAGCCATGGTGGCAACGCTGGGAGCAGCGGTACCCGTTACAGGGATTACAGCGAATGCGGCGACTAAGAAGACAACGGCTACGAAGAATGTCGCACCAAGTTTGAAGGTCAATGCAATTTACAATAATACGACGCAAATTACAGGTACGGCAACCAAGGGCGCTAAGATCACTGTAAAAAGTACGGCCAACGCCAAGAAAAATCTTGGAACTGCAACGGCCTCTAAAACGACGGGAAAGTACACGGTTAAATTAGCCAAGACATTGAAGGCGCAAAGCAATGTTTATGTGTACGCCACGAATCCAACGACTAAGGCTTATTTCTACCGCGTCATCCGTGTTCAGACTGCCAAAACTACTGCCTCTTCAACTAAGAAGACGACTAACAAGACAACCACGACTAAGAAAGTGGCAACTAAATCGACGGCGGCCACTTCGTCATCCAAGAAGACGACTAAATCGTTTAAAGTCAACGCGCCAGCGGGTACTTGGAAGTCGAATACGACTAACAAGTACTCCCAAATCTTAACGTTTAGCGCTAAGAATGGATTTAATCAGACGCTTTATAAGAATGGCAAACAAGTTAAAAAGCTGGTCTCGTATGCGAGTTATAGCATGACTGCCAAGACGCCAACGTTCTGGAAAGTCAGCTATAAGTCAGGCTCGACTACTAAGACCCTATATTTACGCTATACAGCAACTAATAAGTTTACGGTCGTTAATGCTAAAAATCAGATCGTTAAAGTTAAGGTTGGCAATGCCCCAGCAGCTGTTTGGACGTTTACCAAGAAGTGA |
Reference |
Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed] |
Miscellaneous |
Cell fractions[102mU/mL] 29.9 Standard deviation 5.1 Secretion efficiency(%) 1 |