SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0004012

Accession Number SPSED0004012
Secreted Protein Amylase (AmyA)
Uniprot Accession Number A4ULJ3
GenBank Accession Number ABO77965.1
Source Organism of the Secreted Protein Lactobacillus amylovorus NRRL B-4549
Nucleotide Sequence of the Secreted Protein Length = 1434 nt
ATGAAAAAAAAGAAAAGTTTCTGGCTTGTTTCTTTTTTAGTTATAGTAGCTAGTGTTTTCTTTATATCTTTTGGATTTAGCAATCATTCTAAACAAGTTGCTCAAGCGGCTAGTGATACGACATCAACTGATCACTCAAGCAATGATACAGCTGATTCTGTTAGCGACGGTGTTATTTTGCATGCATGGTGCTGGTCGTTCAACACGATTAAAAACAACTTGAAACAGATTCATGACGCCGGCTACACAGCGGTTCAAACTTCACCTGTTAATGAAGTTAAAGTTGGAAATAGCGGGTCTAAGTCATTAAATAACTGGTATTGGCTATATCAGCCAACTAAATATAGTATTGGTAACTATTATTTAGGAACGGAAGCTGAATTTAAGTCAATGTGCGCTGCTGCTAAAGAATATAATATCAGGATCATTGTCGATGCAACTCTGAATGATACAACAAGTGATTATAGTGCAATTTCGGATGAAATTAAAAGTATTCCAAATTGGACACATGGTAACAAACAAATTTCGAATTGGAGTGATCGTGAAGATGTTACTCAAAATTCGTTGTTAGGTTTATATGATTGGAATACTCAAAATTCTCAAGTTCAGACGTATTTGAAGAATTATTTGGAACGCTTGATTTCTGACGGAGCTTCAGGCTTTCGTTATGATGCAGCTAAGCATATTGAACTTCCAAGTCAATATGATGGCAGCTATGGCAGCAATTTCTGGCCAAATATTACTGATAATGGGTCTGAATTTCAGTATGGTGAAGTTTTGCAGGACTCGATTTCAAAAGAATCAGATTATGCTAATTACATGAGTGTTACAGCTTCAAATTACGGCAATACGATTCGCAATGCGTTAAAGAATCGTGATTTTACCGCAAGTACTTTGCAGAATTTCAACATCAGTGTTCCAGCTTCTAAATTAGTAACTTGGGTCGAATCGCATGATAATTATGCTAACGATGATCAAGTTTCGACTTGGATGAATAATAGTGATATTAAATTAGGCTGGGCTGTTGTTGCTTCGCGTTCTGGTAGTGTTCCGCTGTTCTTTGACCGTCCAGTTGATGGTGGTAATGGTACTCGGTTCCCTGGCAGTTCAGAAATTGGTGATGCTGGCAGCAGTTTGTATTATGATAAAGCAGTTGTAGCTGTTAATAAATTCCATAATGCAATGGCTGGTCAATCTGAATATATTTCTAATCCAAATGGCAATACCAAGATTTTTGAAAATGAACGTGGCAGCAAAGGGGTTGTTTTTGCAAACGCTTCCGACAGTTCATATAGTTTGAATGTTAAAACTAGTTTAGCTGATGGGACTTATGAAAACAAGGCTGGTTCAGATGAATTTACCGTTAAAAATGGTTATTTAACCGGTACAATTCAAGGACGTGAAGTTGTTGTTCTTTACGGGGATCCAACAAGC
Amino Acid Sequence of the Secreted Protein Length = 478 aa
MKKKKSFWLVSFLVIVASVFFISFGFSNHSKQVAQAASDTTSTDHSSNDTADSVSDGVILHAWCWSFNTIKNNLKQIHDAGYTAVQTSPVNEVKVGNSGSKSLNNWYWLYQPTKYSIGNYYLGTEAEFKSMCAAAKEYNIRIIVDATLNDTTSDYSAISDEIKSIPNWTHGNKQISNWSDREDVTQNSLLGLYDWNTQNSQVQTYLKNYLERLISDGASGFRYDAAKHIELPSQYDGSYGSNFWPNITDNGSEFQYGEVLQDSISKESDYANYMSVTASNYGNTIRNALKNRDFTASTLQNFNISVPASKLVTWVESHDNYANDDQVSTWMNNSDIKLGWAVVASRSGSVPLFFDRPVDGGNGTRFPGSSEIGDAGSSLYYDKAVVAVNKFHNAMAGQSEYISNPNGNTKIFENERGSKGVVFANASDSSYSLNVKTSLADGTYENKAGSDEFTVKNGYLTGTIQGREVVVLYGDPTS
Expression Host Lactobacillus plantarum WCFS1

Source Organism of the Signal Peptide Lactobacillus plantarum WCFS1
Source Gene of the Signal Peptide hpk9
Signal Peptide Sequence Length = 30 aa
MKKLMIAVTGLAMVATLGAAVPVTGITANA
Type Sec
Yield 0.43 (10^2mU/mL)
Performance (Current Yield / Highest Yield) 0.13
Charge 2
Charge (N-Region) 2
Hydrophobicity 1.29
Hydrophobicity Plot
Hydrophobicity (H-region) 1.85
Signal Peptide Original Protein Sequence MKKLMIAVTGLAMVATLGAAVPVTGITANAATKKTTATKNVAPSLKVNAIYNNTTQITGTATKGAKITVKSTANAKKNLGTATASKTTGKYTVKLAKTLKAQSNVYVYATNPTTKAYFYRVIRVQTAKTTASSTKKTTNKTTTTKKVATKSTAATSSSKKTTKSFKVNAPAGTWKSNTTNKYSQILTFSAKNGFNQTLYKNGKQVKKLVSYASYSMTAKTPTFWKVSYKSGSTTKTLYLRYTATNKFTVVNAKNQIVKVKVGNAPAAVWTFTKK
Signal Peptide Original DNA Sequence ATGAAGAAATTGATGATTGCTGTAACTGGTTTAGCCATGGTGGCAACGCTGGGAGCAGCGGTACCCGTTACAGGGATTACAGCGAATGCGGCGACTAAGAAGACAACGGCTACGAAGAATGTCGCACCAAGTTTGAAGGTCAATGCAATTTACAATAATACGACGCAAATTACAGGTACGGCAACCAAGGGCGCTAAGATCACTGTAAAAAGTACGGCCAACGCCAAGAAAAATCTTGGAACTGCAACGGCCTCTAAAACGACGGGAAAGTACACGGTTAAATTAGCCAAGACATTGAAGGCGCAAAGCAATGTTTATGTGTACGCCACGAATCCAACGACTAAGGCTTATTTCTACCGCGTCATCCGTGTTCAGACTGCCAAAACTACTGCCTCTTCAACTAAGAAGACGACTAACAAGACAACCACGACTAAGAAAGTGGCAACTAAATCGACGGCGGCCACTTCGTCATCCAAGAAGACGACTAAATCGTTTAAAGTCAACGCGCCAGCGGGTACTTGGAAGTCGAATACGACTAACAAGTACTCCCAAATCTTAACGTTTAGCGCTAAGAATGGATTTAATCAGACGCTTTATAAGAATGGCAAACAAGTTAAAAAGCTGGTCTCGTATGCGAGTTATAGCATGACTGCCAAGACGCCAACGTTCTGGAAAGTCAGCTATAAGTCAGGCTCGACTACTAAGACCCTATATTTACGCTATACAGCAACTAATAAGTTTACGGTCGTTAATGCTAAAAATCAGATCGTTAAAGTTAAGGTTGGCAATGCCCCAGCAGCTGTTTGGACGTTTACCAAGAAGTGA
Reference Mathiesen G, Sveen A, Brurberg M B, et al. Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1[J]. BMC Genomics, 2009, 10(1): 425. [PubMed]
Miscellaneous Cell fractions[102mU/mL] 29.9 Standard deviation 5.1 Secretion efficiency(%) 1