SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0005005

Accession Number SPSED0005005
Secreted Protein α-amylase (AmyE)
Uniprot Accession Number -
GenBank Accession Number -
Source Organism of the Secreted Protein Geobacillus stearothermophilus NBRC 12550
Nucleotide Sequence of the Secreted Protein Length = 1 nt
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Amino Acid Sequence of the Secreted Protein Length = 1 aa
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Expression Host Corynebacterium glutamicum

Source Organism of the Signal Peptide Corynebacterium glutamicum R
Source Gene of the Signal Peptide CgR0324
Signal Peptide Sequence Length = 38 aa
MVELSTRINPNERRFVVFIAAFLAMILVASGLATPAYA
Type Sec
Yield 95.0722 (mU/ml)
Performance (Current Yield / Highest Yield) 0.33
Charge 1
Charge (N-Region) 1
Hydrophobicity 0.92
Hydrophobicity Plot
Hydrophobicity (H-region) 2.69
Signal Peptide Original Protein Sequence MVELSTRINPNERRFVVFIAAFLAMILVASGLATPAYAQQENAFVVTLPSASSHNGKPVYESGKKLRVEVGYGVMDDGYTGVITVPEQIDPSSIVADLSGNTALESVTPNGDGTITFQFTDPFPAGIQQGLFWLDVELKEFETSGEQELKWEFPDGSTQTIDIVVKSKDDEFKTVSDYENKAISSDPWLEATVENGVVSVDSAAFLGAEIEYRININTAEGGTFKIKDNLDEGLVFIDGSFNATRKTWDSNGMNKEEATVSFPQQSGSSFTESIDLPANSEFVLLYKAQIKDQSAVDAIVDKLQSEYERKREEAGDDEFATIYFSTSFVNSATINDGSEKAASFAINGSESGRVGPEVNGVFSKTSDFAFAEIEDPETNDFEEVDLTYTLKVDLTPYAGFENTSKGLGGNVVIVDTLPSDIKWRDGESDFLVADGMVLTRENSSEPLTAQQFESDEYVGKYYVDGKNLFINIGQDTTTIVSIDVKATITGVNEVWRSGGNSPMEEARYWGPDNTAVLTYPNDKIIRTAKHSFAVMKDGSSGINDPQKFSKTTTNNGPIEITQGEPALIEFKFTIGAGIGDARKSIIVDKIDHDVFDVSEETLAAIKASITGNYHWNYPIDGDTMDVTIDGDGNLVFAPNDQFPKAANWGAEASAPFTETFTFAVTIPTHPLYGKQTLQVENKASYRGSDFETVFDSGTEATATSYGNEMELRKRVYNEDSKTFVSQLRVELNEDGELINDEFIYAVELIPHGQFKNMVLDVEDFLPAEVDFVGFINANDFNSGNLDRVTTDPVTLTNTNAKATYVAGERKVHIGRDVLTSGQTSTLYFKVKLTDFEKDQPIANVIGNAEATIVPSNDYPLNIVKVDTSNEENKITDTNAIFQIKKGDDVVVDNVYVVNGNLVVSGDNGAHKPVAVKEPGEYTIVEVKAPAGFLLTDQEFNVSITEQGVQTPDQVRINNQPGQDVADPVIGTSVAVEGSDVKVLPVSGGTVIDTVTYEGLTVGQKYILEGELFTKAGVATGIKGSREFIAEAADGSVAVAFEISADQIAEYAGEKLVAFESLFELNDDVKSEDPVAEHRDVEDEAQTFTVDELPVADPVIGTSVAVEGSDVKVLPVSGGTVIDTVTYEGLTVGQKYILEGELFTKAGVATGIKGSREFIAEAADGSVAVAFEISADQIAEYAGEKLVAFESLFELNDDVKSEDPVAEHRDVEDEAQTFTVDELPVADPAIGTTAKVTGTTDKVLPLTGGQVIDSVAYKDLQPNTKYVLQGVIQHVATDGTVTSTDVVASTVFTTGDAPAGEFFVSGNAEVKFNIDQATAAEYAGERLVVFEQLWLADDNGNKTGDEPVAVHEDPTDEAQTFYAGEKPDAIPGIGTTAKVTGSADKVLPLTGGEIVDTVAYKDLQPNTEYVLNGEIQHVAEDGTVTSTGVIATATFTTGAAKSGEFYVSGTTTVTFDIDEQTAIKYAGEKLVVFETLYTAGNPETPVASHVDPEDEAQSFTVAPQPEADVVVEKTVTGPKGAQVEADENALFQITATWTDELGRNFSKTFNVVPGKPVSLEGLPTNTEIYLSETGATTDVKNVKWGDVIWTGEGVVDETGTSKGATVTFTGEGPFEIGLENKTSSNGMIIIPIPIPLFPIDGGSSTNPIQPDPTQPDPKESVDPTNPTPSEGSEAGTSPSKDKGAEQPETVKPRTPSEKVGLAQTGANVAWVAGIAILLLLAGAALIVRGRRKDA
Signal Peptide Original DNA Sequence ATGGTTGAATTATCTACCCGCATCAATCCCAATGAGAGGAGGTTCGTTGTTTTCATTGCTGCCTTCCTGGCGATGATTCTGGTCGCCAGTGGACTTGCAACTCCTGCTTATGCCCAACAGGAAAACGCATTTGTTGTCACACTTCCAAGTGCAAGTTCGCACAACGGCAAACCGGTTTATGAATCGGGCAAGAAACTCCGCGTTGAAGTCGGCTATGGTGTTATGGACGACGGCTACACAGGTGTTATCACAGTTCCCGAGCAAATAGATCCGTCAAGCATTGTCGCCGACCTTAGCGGTAATACCGCGCTGGAAAGCGTTACTCCCAACGGTGACGGCACCATTACCTTCCAGTTCACCGATCCATTCCCCGCAGGTATTCAGCAGGGACTTTTCTGGCTTGATGTTGAACTCAAAGAGTTTGAAACCTCCGGCGAGCAGGAACTGAAATGGGAGTTCCCCGACGGAAGCACCCAAACCATTGACATTGTTGTCAAAAGCAAAGACGACGAGTTCAAAACTGTCTCAGATTATGAAAACAAAGCGATCTCCTCGGACCCTTGGCTTGAGGCCACGGTAGAGAATGGAGTCGTCTCAGTAGACTCTGCTGCGTTCCTCGGAGCAGAGATCGAATATCGTATCAACATCAACACTGCTGAAGGTGGCACCTTCAAAATCAAAGACAACCTTGATGAGGGACTTGTCTTTATCGATGGCTCATTCAATGCAACCCGTAAGACATGGGATAGCAATGGAATGAACAAAGAAGAAGCAACGGTGAGTTTTCCGCAGCAGAGCGGTTCTTCATTTACTGAGAGCATTGACCTTCCTGCAAACTCAGAGTTCGTTCTCCTATACAAGGCACAAATTAAAGACCAGAGTGCAGTAGATGCAATCGTCGACAAGCTGCAATCCGAGTATGAACGCAAACGTGAAGAAGCCGGCGACGATGAGTTCGCCACCATTTATTTCTCCACCAGTTTCGTTAACTCCGCCACCATTAATGATGGCAGCGAGAAAGCAGCCTCTTTTGCCATCAACGGCTCAGAATCTGGCCGAGTCGGCCCAGAAGTTAATGGTGTCTTCTCCAAAACCAGTGACTTCGCCTTTGCAGAAATTGAAGATCCAGAGACAAATGACTTCGAGGAAGTAGACCTCACCTACACACTCAAAGTAGATCTCACCCCATACGCAGGATTTGAAAACACTTCGAAAGGCCTTGGCGGCAACGTAGTCATCGTTGACACCCTGCCCTCAGATATTAAATGGCGAGACGGCGAATCAGACTTCCTCGTAGCCGACGGCATGGTTCTGACCCGTGAAAACTCTTCCGAACCTCTAACTGCCCAGCAGTTTGAGTCTGATGAATACGTGGGTAAGTACTACGTTGATGGAAAAAACCTCTTCATCAACATTGGACAAGACACCACCACTATTGTCTCCATTGACGTAAAAGCCACTATCACAGGTGTCAATGAAGTCTGGCGCAGCGGTGGAAACTCACCAATGGAAGAAGCACGCTACTGGGGACCAGATAATACTGCTGTTCTGACCTACCCAAATGACAAGATCATTCGCACTGCCAAGCATTCATTCGCAGTAATGAAGGACGGCTCCAGCGGAATCAATGATCCACAAAAGTTCAGCAAGACCACCACTAACAACGGCCCTATCGAAATTACCCAGGGCGAGCCTGCACTTATTGAGTTCAAGTTCACTATTGGTGCAGGCATCGGTGATGCTCGAAAATCAATAATCGTAGACAAGATTGACCACGATGTTTTTGATGTCAGTGAAGAGACCCTCGCTGCCATCAAGGCTTCCATTACTGGTAACTATCATTGGAACTACCCAATCGATGGCGACACCATGGACGTCACCATTGATGGAGATGGCAACCTTGTTTTCGCCCCGAATGATCAGTTTCCTAAAGCAGCAAACTGGGGAGCCGAGGCTTCTGCACCGTTTACCGAAACGTTTACATTCGCAGTAACAATCCCAACGCACCCGCTTTACGGTAAACAAACACTTCAAGTTGAAAACAAAGCGAGCTACCGCGGATCAGATTTCGAGACCGTTTTTGACTCCGGAACTGAAGCAACAGCAACTTCATACGGCAACGAAATGGAACTGCGCAAACGCGTTTACAACGAAGACAGCAAAACTTTTGTTAGCCAGCTTCGCGTTGAATTGAACGAAGACGGAGAGCTCATCAATGATGAGTTCATCTACGCTGTTGAGCTGATCCCACACGGACAGTTCAAAAACATGGTCTTGGACGTGGAAGACTTCCTTCCCGCTGAAGTTGATTTTGTAGGCTTCATCAACGCCAACGATTTCAACTCCGGGAATCTAGATCGTGTCACCACAGATCCCGTCACTTTGACCAACACAAATGCAAAAGCAACCTATGTGGCAGGGGAGAGGAAAGTTCACATTGGTCGCGATGTCCTAACATCCGGCCAGACCTCTACCCTCTATTTCAAAGTGAAGCTCACAGACTTTGAAAAGGACCAGCCGATCGCCAATGTGATTGGTAATGCAGAAGCAACCATCGTTCCTTCCAATGATTATCCGTTAAACATCGTCAAGGTAGACACCTCAAACGAGGAAAACAAGATTACCGATACCAACGCGATCTTCCAGATCAAAAAGGGTGACGATGTAGTGGTTGACAACGTTTACGTTGTCAACGGAAACCTCGTTGTCTCCGGCGACAACGGTGCACACAAACCAGTTGCTGTTAAAGAACCAGGCGAATACACCATCGTTGAAGTCAAAGCACCAGCTGGATTCCTTCTAACTGACCAAGAATTCAACGTTTCAATCACTGAGCAAGGCGTTCAAACACCTGATCAGGTTCGAATCAACAACCAACCTGGCCAGGACGTAGCTGATCCTGTCATTGGTACTTCGGTGGCAGTTGAGGGCTCTGATGTTAAGGTGTTGCCGGTTTCTGGTGGCACTGTTATCGATACTGTTACCTACGAGGGTTTGACTGTTGGTCAGAAGTACATCCTTGAAGGTGAGTTGTTCACCAAGGCGGGTGTGGCAACCGGTATCAAGGGATCTCGGGAGTTTATTGCTGAGGCCGCTGATGGTTCTGTGGCTGTAGCGTTTGAGATTTCCGCTGATCAGATTGCTGAGTACGCGGGTGAGAAGCTTGTTGCTTTTGAGTCTCTGTTTGAGCTGAATGATGATGTTAAGTCTGAGGATCCTGTTGCTGAGCATAGGGATGTTGAAGATGAGGCACAGACCTTTACCGTGGATGAGCTCCCTGTAGCTGATCCTGTCATTGGTACTTCGGTGGCAGTTGAGGGCTCTGATGTTAAGGTGTTGCCGGTTTCTGGTGGCACTGTTATCGATACTGTTACCTACGAGGGTTTGACTGTTGGTCAGAAGTACATCCTTGAAGGTGAGTTGTTCACCAAGGCGGGTGTGGCAACCGGTATCAAGGGATCTCGGGAGTTTATTGCTGAGGCCGCTGATGGTTCTGTGGCTGTAGCGTTTGAGATTTCCGCTGATCAGATTGCTGAGTACGCGGGTGAGAAGCTTGTTGCTTTTGAGTCTCTGTTTGAGCTGAATGATGATGTTAAGTCTGAGGATCCTGTTGCTGAGCATAGGGATGTTGAAGATGAGGCACAGACCTTTACCGTGGATGAGCTCCCTGTAGCTGATCCTGCAATCGGTACGACTGCTAAGGTCACCGGCACAACCGATAAGGTTCTTCCACTAACCGGCGGTCAAGTTATTGACTCCGTTGCGTACAAGGATCTGCAGCCAAACACCAAGTACGTCCTGCAAGGTGTCATCCAGCATGTTGCTACAGACGGCACTGTCACCTCAACTGATGTAGTTGCTTCCACTGTGTTCACCACTGGGGATGCACCTGCCGGTGAATTCTTCGTGTCAGGAAATGCTGAAGTTAAGTTCAACATTGATCAGGCTACTGCTGCAGAATATGCAGGTGAAAGGCTTGTCGTATTCGAACAGCTGTGGCTTGCTGATGACAACGGTAACAAGACTGGTGATGAACCAGTAGCTGTCCACGAGGACCCTACTGATGAAGCACAGACCTTCTACGCTGGTGAAAAGCCTGACGCAATCCCTGGCATTGGCACCACTGCAAAGGTCACTGGATCTGCAGATAAGGTTCTTCCACTAACTGGCGGCGAAATTGTTGACACCGTTGCGTACAAGGACTTGCAGCCAAACACCGAATATGTTTTGAACGGTGAGATTCAGCATGTTGCTGAAGACGGCACTGTTACCTCCACGGGTGTCATTGCTACCGCTACCTTCACCACTGGTGCAGCGAAGAGCGGCGAGTTCTATGTTTCCGGCACCACCACGGTTACATTCGACATTGACGAGCAGACTGCCATTAAGTACGCAGGTGAGAAGCTTGTTGTCTTTGAAACTCTCTACACTGCTGGAAACCCAGAAACTCCTGTGGCTTCCCACGTAGACCCAGAAGATGAGGCTCAGTCCTTCACTGTTGCGCCACAGCCTGAAGCCGATGTCGTTGTTGAAAAGACTGTCACCGGACCAAAGGGTGCTCAGGTTGAAGCCGATGAAAATGCGCTGTTCCAGATCACCGCAACTTGGACCGATGAACTGGGACGCAATTTCTCTAAGACCTTCAACGTAGTTCCAGGAAAGCCTGTCTCTCTTGAAGGCCTTCCAACCAACACCGAGATTTACTTGTCTGAAACTGGTGCCACAACTGACGTCAAGAACGTCAAGTGGGGCGACGTAATTTGGACTGGGGAAGGCGTTGTTGATGAAACCGGCACGTCAAAGGGCGCGACTGTCACCTTCACTGGTGAAGGTCCATTCGAAATTGGCCTCGAGAACAAGACCAGCTCCAACGGTATGATCATCATCCCAATTCCGATCCCGTTGTTCCCAATTGATGGTGGTTCGTCGACCAACCCAATTCAGCCTGACCCAACTCAGCCAGATCCAAAGGAATCGGTTGACCCAACCAACCCGACTCCTTCGGAAGGATCCGAGGCAGGAACCTCACCAAGCAAGGACAAAGGAGCAGAGCAGCCTGAAACTGTGAAGCCCCGTACTCCTTCTGAAAAGGTTGGACTTGCTCAGACTGGAGCAAACGTAGCGTGGGTTGCAGGAATTGCAATTCTGCTTCTGCTTGCAGGTGCTGCTTTAATCGTCCGTGGACGTAGGAAAGACGCTTAA
Reference Watanabe K, Tsuchida Y, Okibe N, et al. Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences[J]. Microbiology-Sgm, 2009, 155: 741-750. [PubMed]
Miscellaneous -