SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0006008

Accession Number SPSED0006008
Secreted Protein Bacillus protease novo type (BPN')
Uniprot Accession Number P00782
GenBank Accession Number AAB05345.1
Source Organism of the Secreted Protein Bacillus amyloliquefaciens ATCC 23844
Nucleotide Sequence of the Secreted Protein Length = 1131 nt
TGGATCAGTTTGCTGTTTGCTTTAGCGTTAATCTTTACGATGGCGTTCGGCAGCACATCCTCTGCCCAGGCGGCAGGGAAATCAAACGGGGAAAAGAAATATATTGTCGGGTTTAAACAGACAATGAGCACGATGAGCGCCGCTAAGAAGAAAGATGTCATTTCTGAAAAAGGCGGGAAAGTGCAAAAGCAATTCAAATATGTAGACGCAGCTTCAGCTACATTAAACGAAAAAGCTGTAAAAGAATTGAAAAAAGACCCGAGCGTCGCTTACGTTGAAGAAGATCACGTAGCACATGCGTACGCGCAGTCCGTGCCTTACGGCGTATCACAAATTAAAGCCCCTGCTCTGCACTCTCAAGGCTACACTGGATCAAATGTTAAAGTAGCGGTTATCGACAGCGGTATCGATTCTTCTCATCCTGATTTAAAGGTAGCAGGCGGAGCCAGCATGGTTCCTTCTGAAACAAATCCTTTCCAAGACAACAACTCTCACGGAACTCACGTTGCCGGCACAGTTGCGGCTCTTAATAACTCAATCGGTGTATTAGGCGTTGCGCCAAGCGCATCACTTTACGCTGTAAAAGTTCTCGGTGCTGACGGTTCCGGCCAATACAGCTGGATCATTAACGGAATCGAGTGGGCGATCGCAAACAATATGGACGTTATTAACATGAGCCTCGGCGGACCTTCTGGTTCTGCTGCTTTAAAAGCGGCAGTTGATAAAGCCGTTGCATCCGGCGTCGTAGTCGTTGCGGCAGCCGGTAACGAAGGCACTTCCGGCAGCTCAAGCACAGTGGGCTACCCTGGTAAATACCCTTCTGTCATTGCAGTAGGCGCTGTTGACAGCAGCAACCAAAGAGCATCTTTCTCAAGCGTAGGACCTGAGCTTGATGTCATGGCACCTGGCGTATCTATCCAAAGCACGCTTCCTGGAAACAAATACGGGGCGTACAACGGTACGTCAATGGCATCTCCGCACGTTGCCGGAGCGGCTGCTTTGATTCTTTCTAAGCACCCGAACTGGACAAACACTCAAGTCCGCAGCAGTTTAGAAAACACCACTACAAAACTTGGTGATTCTTTCTACTATGGAAAAGGGCTGATCAACGTACAGGCGGCAGCTCAGTAA
Amino Acid Sequence of the Secreted Protein Length = 382 aa
MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ
Expression Host Bacillus subtilis TEB1030

Source Organism of the Signal Peptide Bacillus subtilis 168
Source Gene of the Signal Peptide walM
Signal Peptide Sequence Length = 28 aa
MLKKVILAAFILVGSTLGAFSFSSDASA
Type Sec
Yield 2.87 (U/ml)
Performance (Current Yield / Highest Yield) 0.47
Charge 1
Charge (N-Region) 2
Hydrophobicity 1.25
Hydrophobicity Plot
Hydrophobicity (H-region) 3.1
Signal Peptide Original Protein Sequence MLKKVILAAFILVGSTLGAFSFSSDASAKHVNGNITWYNGVGKKGSSGKKLGHWDCATKIGFDVPRNGTKIRAYAKAKPKKVITVYKNDVGRMPNAVLDVSPKAFKALGYPLSKGKVAGHYSY
Signal Peptide Original DNA Sequence ATGTTGAAGAAAGTCATTTTAGCCGCTTTTATCTTAGTAGGAAGTACTTTGGGAGCTTTTAGTTTTTCATCAGATGCCAGTGCGAAACATGTGAACGGAAATATTACTTGGTATAATGGAGTCGGGAAAAAAGGCTCTTCAGGTAAGAAACTTGGACATTGGGATTGTGCGACCAAAATCGGATTTGATGTTCCTAGAAACGGGACAAAAATCAGAGCTTATGCAAAAGCGAAGCCTAAAAAGGTGATTACGGTTTATAAAAATGATGTTGGCAGAATGCCTAATGCAGTGCTGGATGTTAGCCCTAAAGCGTTTAAAGCGCTTGGATACCCATTAAGCAAAGGTAAAGTAGCGGGGCATTACAGCTATTAA
Reference Degering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. [PubMed]
Miscellaneous -