SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0010003

Accession Number SPSED0010003
Secreted Protein CotA-laccase
Uniprot Accession Number A0A0G2QNK1
GenBank Accession Number AGO57931.1
Source Organism of the Secreted Protein Bacillus pumilus W3
Nucleotide Sequence of the Secreted Protein Length = 1533 nt
ATGAACCTAGAAAAATTTGTTGACGAGCTGCCAATTCCAGAAGTCGCAGAGCCCGTCAAAAAAAACCCAAGACAAACGTATTATGAAATCGCTATGGAGGAGGTGTTCCTAAAAGTTCATAGTGATCTGCCCCCAACCAAGTTATGGACCTATAATGGCGGTTTGCCTGGTCCAACCATTAAAGCGAATCGAAATGAAAAGGTCAAAGTGAAATGGATGAACAAATTGCCGCTGAAACACTTCCTGCCTGTCGATCATACCATTCACGCTGGACACCATGATGAACCCGAAGTCAAAACAGTCGTTCACCTGCATGGGGGCGTGACACCTGCAAGCAGTGACGGTTATCCAGAGGCTTGGTTTTCGCGAGACTTTGAAGCGACCGGCCCCTTCTTTGAACGAGAGGTTTACGAATACCCTAATCACCAGCAAGCATGCACATTGTGGTATCACGATCATGCCATGGCATTAACACGATTAAATGTATACGCCGGATTAGCTGGCTTTTATTTGATCTCAGATGCGTTTGAAAAATCACTCGAATTACCGAAAGATGAATATGATATCCCTTTAATGATCATGGACCGGACATTCCAGGAGGATGGCGCACTATTTTATCCAAGTAGACCAAATAACACACCAGAAGATAGTGACCTTCCAGATCCCTCTATCGTGCCATTTTTTTGCGGGGAAACGATTTTAGTCAATGGAAAAGTATGGCCATATTTAGAAGTAGAGCCTCGAAAATATCGTTTTCGTATCTTAAACGCGTCCAATACAAGAACTTACGAGCTTCATCTAGATAACGACGCCACAATCTTACAAATTGGATCTGATGGCGGCTTTTTGCCAAGACCTGTTCATCACCAATCTTTTAGCATTGCACCTGCTGAACGTTTTGATGTCATCATCGACTTCTCAGCTTACGAAAACCAAACGATTGTTTTAAAAAATAAGGCAGGCTGCGGTCAGGAAGTCAATCCAGAAACAGATGCGAACATTATGCAATTTAAAGTCACTCGACCGCTCAAAGGAAGAGCAGCTAAAACATTACGTCCGATCTTCAAACCACTTCCACCACTCCGGCCGAGCCGAGCTGATAACGAGCGAACGCTGACCCTTACTGGCACACAAGATAAATATGGGCGCCCTATTTTATTACTAGATAACCAGTTTTGGAATGATCCTGTTACGGAAAATCCTCGACTTGGCAGTGTAGAGGTATGGAACATCGTTAACCCAACAAGGGGCACACACCCTATTCATTTACATCTTGTTCAATTTCGGGTGATTGATAGAAGACCATTCGATACAGACATCTATCAATCAACAGGTGAAATCGTGTACACGGGACCAAATGAAGCGCCTCCTTTGCATGAACAAGGATACAAGGATACAATTCAGGCGCATGCCGGTGAAGTCATTCGCATCATCGCTCGGTTTGTCCCATATAGCGGACGGTATGTGTGGCATTGTCACATATTAGAACACGAGGATTATGACATGATGCGGCCGATGGATATCATCCAGTAA
Amino Acid Sequence of the Secreted Protein Length = 510 aa
MNLEKFVDELPIPEVAEPVKKNPRQTYYEIAMEEVFLKVHSDLPPTKLWTYNGGLPGPTIKANRNEKVKVKWMNKLPLKHFLPVDHTIHAGHHDEPEVKTVVHLHGGVTPASSDGYPEAWFSRDFEATGPFFEREVYEYPNHQQACTLWYHDHAMALTRLNVYAGLAGFYLISDAFEKSLELPKDEYDIPLMIMDRTFQEDGALFYPSRPNNTPEDSDLPDPSIVPFFCGETILVNGKVWPYLEVEPRKYRFRILNASNTRTYELHLDNDATILQIGSDGGFLPRPVHHQSFSIAPAERFDVIIDFSAYENQTIVLKNKAGCGQEVNPETDANIMQFKVTRPLKGRAAKTLRPIFKPLPPLRPSRADNERTLTLTGTQDKYGRPILLLDNQFWNDPVTENPRLGSVEVWNIVNPTRGTHPIHLHLVQFRVIDRRPFDTDIYQSTGEIVYTGPNEAPPLHEQGYKDTIQAHAGEVIRIIARFVPYSGRYVWHCHILEHEDYDMMRPMDIIQ
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide amyX
Signal Peptide Sequence Length = 30 aa
MVSIRRSFEAYVDDMNIITVLIPAEQKEIM
Type Tat
Yield 27.8 (U/ml)
Performance (Current Yield / Highest Yield) 0.12
Charge -2
Charge (N-Region) -1
Hydrophobicity 0.28
Hydrophobicity Plot
Hydrophobicity (H-region) 1.96
Signal Peptide Original Protein Sequence MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLHGYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA
Signal Peptide Original DNA Sequence ATGGTCAGCATCCGCCGCAGCTTCGAAGCGTATGTCGATGACATGAATATCATTACTGTTCTGATTCCTGCTGAACAAAAGGAAATCATGACACCGCCGTTTCGGCTTGAGACAGAAATAACAGATTTTCCTCTGGCTGTCAGGGAGGAATACTCCCTTGAAGCAAAATACAAGTACGTCTGCGTATCCGACCATCCTGTGACATTTGGAAAAATCCATTGCGTCAGAGCATCCAGCGGCCACAAAACGGATCTCCAAATTGGCGCGGTCATCCGGACGGCAGCGTTTGATGACGAATTTTATTATGACGGAGAGCTGGGCGCCGTTTATACCGCGGATCATACCGTATTTAAAGTATGGGCGCCTGCTGCAACCTCAGCTGCTGTCAAGCTTTCACACCCCAATAAAAGCGGGCGCACATTCCAAATGACTCGCTTGGAAAAAGGCGTCTATGCCGTTACGGTCACAGGTGACCTTCACGGATATGAGTATTTGTTTTGCATCTGCAACAATTCAGAATGGATGGAAACAGTTGACCAGTATGCCAAGGCTGTGACTGTAAATGGAGAGAAGGGCGTCGTCTTGCGCCCGGATCAAATGAAATGGACTGCTCCTCTTAAACCATTCTCACACCCTGTGGATGCCGTCATCTATGAGACGCATCTTCGCGACTTCTCCATCCATGAAAACAGCGGCATGATAAACAAGGGAAAATACTTAGCGCTGACGGAAACTGATACACAAACCGCAAATGGCAGTTCTTCGGGATTAGCGTATGTAAAAGAGCTTGGTGTGACACATGTGGAGCTTCTGCCGGTGAATGATTTTGCCGGAGTTGATGAAGAGAAGCCGCTTGATGCATACAATTGGGGATATAACCCGCTTCATTTCTTTGCCCCGGAGGGAAGCTATGCCTCAAATCCTCATGATCCTCAAACGAGAAAAACAGAGCTGAAACAAATGATCAATACCCTGCATCAGCACGGTCTGCGAGTCATTCTGGATGTTGTTTTTAACCATGTGTATAAGAGGGAGAATTCCCCCTTTGAAAAGACAGTGCCCGGTTATTTTTTCCGGCACGACGAATGTGGGATGCCATCAAACGGCACCGGCGTTGGCAATGATATTGCATCAGAAAGAAGGATGGCAAGAAAATTCATTGCGGATTGCGTGGTCTATTGGCTTGAAGAATACAATGTTGACGGCTTCCGCTTTGATCTCCTCGGGATTTTAGATATTGACACCGTGCTTTATATGAAAGAGAAAGCAACTAAGGCAAAGCCCGGAATCCTGCTTTTTGGAGAAGGGTGGGACCTGGCTACACCGCTGCCGCATGAACAGAAAGCTGCTTTGGCGAACGCGCCAAGAATGCCGGGCATCGGCTTTTTTAATGATATGTTTCGTGACGCTGTAAAAGGGAACACCTTTCACCTTAAGGCAACAGGGTTTGCGCTCGGCAACGGTGAATCAGCACAAGCTGTGATGCATGGAATTGCCGGGTCTTCCGGATGGAAGGCATTAGCACCGATTGTTCCGGAACCAAGCCAGTCCATCAATTATGTCGAATCACACGACAATCACACCTTTTGGGATAAAATGAGCTTTGCGCTTCCTCAAGAAAATGACAGCCGAAAGCGAAGCAGGCAAAGGCTTGCAGCCGCGATTATTTTGCTTGCCCAAGGGGTGCCGTTTATTCACAGCGGCCAGGAATTTTTCCGGACGAAGCAGGGAGTGGAAAACAGCTATCAATCCAGTGACAGCATCAACCAGCTCGACTGGGATCGCCGTGAAACATTCAAAGAAGATGTTCACTATATCCGCAGGCTGATCTCGCTGAGAAAAGCGCATCCTGCATTCCGTCTTAGGTCCGCTGCAGACATCCAGCGCCATCTTGAATGCTTGACGCTAAAAGAACACCTTATCGCATACAGGCTTTATGATCTTGACGAGGTTGACGAATGGAAAGATATCATTGTTATCCATCACGCGAGTCCAGACTCCGTCGAGTGGAGGCTGCCAAACGACATACCTTATCGGCTTTTATGTGATCCATCAGGATTTCAGGAAGACCCAACAGAAATCAAGAAAACGGTTGCAGTAAACGGCATCGGAACGGTTATCTTATATTTAGCATCAGATCTTAAGAGTTTTGCTTGA
Reference Guan Z B, Shui Y, Song C M, et al. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater[J]. Environmental Science and Pollution Research, 2015, 22(12): 9515-9523. [PubMed]
Miscellaneous -