SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0010004

Accession Number SPSED0010004
Secreted Protein CotA-laccase
Uniprot Accession Number A0A0G2QNK1
GenBank Accession Number AGO57931.1
Source Organism of the Secreted Protein Bacillus pumilus W3
Nucleotide Sequence of the Secreted Protein Length = 1533 nt
ATGAACCTAGAAAAATTTGTTGACGAGCTGCCAATTCCAGAAGTCGCAGAGCCCGTCAAAAAAAACCCAAGACAAACGTATTATGAAATCGCTATGGAGGAGGTGTTCCTAAAAGTTCATAGTGATCTGCCCCCAACCAAGTTATGGACCTATAATGGCGGTTTGCCTGGTCCAACCATTAAAGCGAATCGAAATGAAAAGGTCAAAGTGAAATGGATGAACAAATTGCCGCTGAAACACTTCCTGCCTGTCGATCATACCATTCACGCTGGACACCATGATGAACCCGAAGTCAAAACAGTCGTTCACCTGCATGGGGGCGTGACACCTGCAAGCAGTGACGGTTATCCAGAGGCTTGGTTTTCGCGAGACTTTGAAGCGACCGGCCCCTTCTTTGAACGAGAGGTTTACGAATACCCTAATCACCAGCAAGCATGCACATTGTGGTATCACGATCATGCCATGGCATTAACACGATTAAATGTATACGCCGGATTAGCTGGCTTTTATTTGATCTCAGATGCGTTTGAAAAATCACTCGAATTACCGAAAGATGAATATGATATCCCTTTAATGATCATGGACCGGACATTCCAGGAGGATGGCGCACTATTTTATCCAAGTAGACCAAATAACACACCAGAAGATAGTGACCTTCCAGATCCCTCTATCGTGCCATTTTTTTGCGGGGAAACGATTTTAGTCAATGGAAAAGTATGGCCATATTTAGAAGTAGAGCCTCGAAAATATCGTTTTCGTATCTTAAACGCGTCCAATACAAGAACTTACGAGCTTCATCTAGATAACGACGCCACAATCTTACAAATTGGATCTGATGGCGGCTTTTTGCCAAGACCTGTTCATCACCAATCTTTTAGCATTGCACCTGCTGAACGTTTTGATGTCATCATCGACTTCTCAGCTTACGAAAACCAAACGATTGTTTTAAAAAATAAGGCAGGCTGCGGTCAGGAAGTCAATCCAGAAACAGATGCGAACATTATGCAATTTAAAGTCACTCGACCGCTCAAAGGAAGAGCAGCTAAAACATTACGTCCGATCTTCAAACCACTTCCACCACTCCGGCCGAGCCGAGCTGATAACGAGCGAACGCTGACCCTTACTGGCACACAAGATAAATATGGGCGCCCTATTTTATTACTAGATAACCAGTTTTGGAATGATCCTGTTACGGAAAATCCTCGACTTGGCAGTGTAGAGGTATGGAACATCGTTAACCCAACAAGGGGCACACACCCTATTCATTTACATCTTGTTCAATTTCGGGTGATTGATAGAAGACCATTCGATACAGACATCTATCAATCAACAGGTGAAATCGTGTACACGGGACCAAATGAAGCGCCTCCTTTGCATGAACAAGGATACAAGGATACAATTCAGGCGCATGCCGGTGAAGTCATTCGCATCATCGCTCGGTTTGTCCCATATAGCGGACGGTATGTGTGGCATTGTCACATATTAGAACACGAGGATTATGACATGATGCGGCCGATGGATATCATCCAGTAA
Amino Acid Sequence of the Secreted Protein Length = 510 aa
MNLEKFVDELPIPEVAEPVKKNPRQTYYEIAMEEVFLKVHSDLPPTKLWTYNGGLPGPTIKANRNEKVKVKWMNKLPLKHFLPVDHTIHAGHHDEPEVKTVVHLHGGVTPASSDGYPEAWFSRDFEATGPFFEREVYEYPNHQQACTLWYHDHAMALTRLNVYAGLAGFYLISDAFEKSLELPKDEYDIPLMIMDRTFQEDGALFYPSRPNNTPEDSDLPDPSIVPFFCGETILVNGKVWPYLEVEPRKYRFRILNASNTRTYELHLDNDATILQIGSDGGFLPRPVHHQSFSIAPAERFDVIIDFSAYENQTIVLKNKAGCGQEVNPETDANIMQFKVTRPLKGRAAKTLRPIFKPLPPLRPSRADNERTLTLTGTQDKYGRPILLLDNQFWNDPVTENPRLGSVEVWNIVNPTRGTHPIHLHLVQFRVIDRRPFDTDIYQSTGEIVYTGPNEAPPLHEQGYKDTIQAHAGEVIRIIARFVPYSGRYVWHCHILEHEDYDMMRPMDIIQ
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide pbtQ
Signal Peptide Sequence Length = 29 aa
MKKFALLFIALVTAVVISACGNQSTSSKG
Type Lip
Yield 23.7 (U/ml)
Performance (Current Yield / Highest Yield) 0.1
Charge 3
Charge (N-Region) 2
Hydrophobicity 0.9
Hydrophobicity Plot
Hydrophobicity (H-region) 2.75
Signal Peptide Original Protein Sequence MKKFALLFIALVTAVVISACGNQSTSSKGSDTKKEQITVKHQLDKNGTKVPKNPKKVVVFDFGSLDTLDKLGLDDIVAGLPKQVLPKYLSKFKDDKYADVGSLKEPDFDKVAELDPDLIIISARQSESYKEFSKIAPTIYLGVDTAKYMESFKSDAETIGKIFDKEDKVKDELANIDHSIADVKKTAEKLNKNGLVIMANDGKISAFGPKSRYGLIHDVFGVAPADQNIKASTHGQSVSYEYISKTNPDYLFVIDRGTAIGETSSTKQVVENDYVKNVNAVKNGHVIYLDSATWYLSGGGLESMTQMIKEVKDGLEK
Signal Peptide Original DNA Sequence ATGAAAAAGTTCGCGTTACTATTCATCGCTTTGGTCACTGCCGTTGTCATTTCTGCATGCGGAAACCAAAGCACAAGCAGCAAAGGTTCTGATACAAAGAAAGAACAAATCACAGTGAAACACCAGCTCGACAAAAACGGCACAAAAGTACCGAAGAACCCTAAAAAAGTTGTCGTATTTGACTTTGGAAGCTTAGATACATTAGATAAACTAGGACTTGATGATATAGTAGCGGGCCTGCCGAAACAAGTCCTTCCTAAATATCTGTCCAAATTCAAGGATGACAAATACGCTGATGTCGGCAGCTTAAAAGAGCCAGACTTCGACAAAGTGGCAGAATTAGATCCTGATCTGATCATTATTTCAGCAAGACAATCTGAGTCTTACAAAGAATTCTCTAAAATTGCGCCGACGATTTACCTTGGTGTAGATACAGCGAAGTACATGGAATCATTTAAATCAGACGCTGAAACAATCGGTAAAATCTTTGATAAAGAAGACAAAGTGAAAGATGAGCTCGCAAACATTGATCACTCAATCGCAGATGTAAAGAAAACCGCTGAAAAGCTTAACAAAAACGGTCTCGTCATCATGGCGAACGATGGAAAAATCAGCGCGTTCGGCCCTAAATCAAGATACGGCCTGATCCATGACGTATTCGGCGTGGCTCCGGCTGATCAAAACATCAAAGCGTCTACGCACGGACAAAGTGTTTCTTACGAGTACATTTCAAAAACAAACCCTGATTACTTGTTTGTTATTGACCGCGGTACAGCAATCGGAGAAACATCATCTACAAAACAAGTCGTGGAAAACGATTATGTGAAAAACGTAAACGCAGTGAAAAATGGTCATGTCATCTACCTTGATTCTGCTACTTGGTATTTATCAGGAGGCGGACTTGAGTCTATGACGCAAATGATTAAAGAAGTGAAAGACGGTTTAGAAAAGTAA
Reference Guan Z B, Shui Y, Song C M, et al. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater[J]. Environmental Science and Pollution Research, 2015, 22(12): 9515-9523. [PubMed]
Miscellaneous -