SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0011028

Accession Number SPSED0011028
Secreted Protein Nattokinase
Uniprot Accession Number Q548F3
GenBank Accession Number AAK54130.1
Source Organism of the Secreted Protein Bacillus subtilis natto
Nucleotide Sequence of the Secreted Protein Length = 1146 nt
GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATCTTTACGATGGCGTTCAGCAACATGTCTGCGCAGGCTGCCGGAAAAAGCAGTACAGAAAAGAAATACATTGTCGGATTTAAGCAGACAATGAGTGCCATGAGTTCCGCCAAGAAAAAGGATGTTATTTCTGAAAAAGGCGGAAAGGTTCAAAAGCAATTTAAGTATGTTAACGCGGCCGCAGCAACATTGGATGAAAAAGCTGTAAAAGAATTGAAAAAAGATCCGAGCGTTGCATATGTGGAAGAAGATCATATTGCACATGAATATGCGCAATCTGTTCCTTATGGCATTTCTCAAATTAAAGCGCCGGCTCTTCACTCTCAAGGCTACACAGGCTCTAACGTAAAAGTAGCTGTTATCGACAGCGGAATTGACTCTTCTCATCCTGACTTAAACGTCAGAGGCGGAGCAAGCTTCGTTCCTTCTGAAACAAACCCATACCAGGACGGCAGTTCTCACGGTACGCATGTCGCCGGTACGATTGCCGCTCTTAATAACTCAATCGGTGTTCTGGGCGTAGCGCCAAGCGCATCATTATATGCAGTAAAAGTGCTTGATTCAACAGGAAGCGGCCAATATAGCTGGATTATTAACGGCATTGAGTGGGCCATTTCCAACAATATGGATGTTATCAACATGAGCCTTGGCGGACCTACTGGTTCTACAGCGCTGAAAACAGTAGTTGATAAAGCGGTTTCCAGCGGTATCGTCGTTGCTGCCGCAGCCGGAAACGAAGGTTCATCCGGAAGCACAAGCACAGTCGGCTACCCTGCAAAATATCCTTCTACTATTGCAGTAGGTGCGGTAAACAGCAGCAACCAAAGAGCTTCATTCTCCAGCGTAGGTTCTGAGCTTGATGTAATGGCTCCTGGCGTGTCCATCCAAAGCACACTTCCTGGAGGCACTTACGGCGCTTATAACGGAACGTCCATGGCGACTCCTCACGTTGCCGGAGCAGCAGCGCTAATTCTTTCTAAGCACCCGACTTGGACAAACGCGCAAGTCCGTGATCGTTTAGAAAGCACTGCAACATATCTTGGAAACTCTTTCTACTATGGAAAAGGGTTAATCAACGTACAAGCAGCTGCACAATAA
Amino Acid Sequence of the Secreted Protein Length = 381 aa
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
Expression Host Bacillus licheniformis △0F-3

Source Organism of the Signal Peptide Bacillus licheniformis WX-02
Source Gene of the Signal Peptide phoD
Signal Peptide Sequence Length = 46 aa
MKKLSEESLKDNTFDRRRFIQGAGKIAGLSLGLAIAQSMGAMEVNA
Type Tat
Yield 5.73 (FU/mL)
Performance (Current Yield / Highest Yield) 0.18
Charge 2
Charge (N-Region) 2
Hydrophobicity -0.17
Hydrophobicity Plot
Hydrophobicity (H-region) 0.7
Signal Peptide Original Protein Sequence MKKLSEESLKDNTFDRRRFIQGAGKIAGLSLGLAIAQSMGAMEVNAQSMGAMEVNAAPRFSEYPFTLGVASGDPLSDSVVLWTRLAPDPLNGGGMPNEAVSVKWELAEDERFRRVVKRGTEKATPHLAHSVHAEVSGLKPNHVYYYRFKCGNQLSPVGKTKTLPAPGADVAKFTFAFASCQQYEHGYYTAYQHMAKEKLDLVFHLGDYIYEYGPNEYVSKTGNVRTHSGPEIMSLLDYRNRHAQYRSDANLKAAHAAFPWVVTWDDHEVENNYANVIPEKGQSVEAFIKRRAAAYQAYYEHMPLRRQSLPNGPDMQLYRNFSYGNLADISVLDTRQYRDDQANGDGNKPPSDESNDPKRTLLGAEQEAWLFDNLSRSEAHWNIIAQQIFFAQWNFGTSAAPIYSMDSWDGYPAQRKRVIDFIKSQKLNNIVVLTGDVHASWANNLLVDFDNPKSDIFGVEFVGTSITSGGNGADKRADTDKILSNNPHIKFFNDYRGYVRCTVTPEEWRTDYRVVPYVTEPGAAVSTRASYVYHKDYTGLKRTASNIVPGGVKKSNEVEEDRFLAHTRAHQKQVKQKEKKVTQ
Signal Peptide Original DNA Sequence ATGACACATGAAGGACCGCTGGAGGAATGGATGAAAAAACTGAGCGAGGAAAGCCTCAAGGACAATACGTTTGACCGCCGCCGCTTTATTCAAGGGGCCGGCAAAATAGCCGGGCTTTCGCTCGGACTTGCGATCGCGCAATCGATGGGGGCAATGGAAGTCAATGCAGCACCGAGGTTCTCCGAATATCCGTTTACACTTGGCGTTGCATCGGGAGATCCGCTTTCTGACAGCGTCGTATTGTGGACAAGGCTGGCGCCCGATCCGCTAAACGGGGGCGGGATGCCAAATGAAGCTGTATCCGTGAAATGGGAGCTCGCAGAAGACGAACGCTTCCGCCGTGTCGTCAAACGGGGAACCGAAAAAGCAACACCTCACCTTGCACATTCGGTACATGCCGAGGTATCCGGGCTGAAACCGAATCATGTATACTATTACCGCTTTAAATGCGGCAACCAGCTGAGTCCTGTCGGCAAGACAAAGACGCTTCCGGCGCCCGGGGCAGATGTTGCTAAATTCACATTCGCTTTTGCTTCATGCCAGCAGTACGAACACGGCTATTATACCGCCTATCAGCATATGGCAAAGGAAAAGCTCGATCTTGTTTTTCATCTCGGCGACTATATTTATGAGTACGGTCCGAATGAATATGTTTCGAAAACAGGAAATGTCAGAACACACAGCGGTCCTGAAATCATGTCACTGCTCGATTACCGAAACCGTCATGCCCAATACCGTTCAGATGCAAACCTGAAAGCTGCGCATGCCGCCTTCCCGTGGGTTGTCACATGGGACGACCATGAAGTGGAGAACAACTATGCGAATGTCATCCCGGAAAAAGGCCAGTCAGTTGAAGCGTTTATTAAACGGCGCGCCGCCGCATACCAAGCTTATTACGAGCATATGCCGCTCCGCCGCCAATCCTTGCCGAACGGTCCTGATATGCAATTGTACCGGAATTTTTCCTACGGCAATTTAGCTGACATTAGCGTGCTGGATACCCGTCAGTATCGCGATGACCAGGCTAACGGCGATGGCAATAAGCCGCCTTCTGATGAATCGAATGATCCGAAGCGGACGCTGCTTGGAGCGGAGCAGGAGGCTTGGCTGTTTGACAATCTGAGTCGCTCCGAGGCGCACTGGAACATTATCGCACAGCAGATTTTCTTTGCGCAGTGGAACTTTGGGACAAGCGCGGCGCCGATTTACAGCATGGATTCGTGGGACGGATATCCCGCTCAGCGCAAGCGGGTGATCGACTTCATCAAATCTCAAAAACTGAACAACATCGTCGTCCTTACCGGTGATGTGCATGCCAGCTGGGCAAATAATCTGCTCGTTGATTTTGACAATCCGAAATCGGACATCTTTGGCGTGGAGTTCGTCGGGACGTCGATTACATCGGGAGGAAACGGCGCTGATAAAAGAGCGGATACGGATAAGATTTTATCGAACAATCCTCATATAAAGTTTTTCAATGATTACCGCGGCTATGTGCGCTGTACGGTCACCCCGGAAGAATGGCGGACAGATTACCGCGTCGTCCCGTATGTGACAGAGCCGGGTGCGGCGGTTTCGACAAGAGCTTCCTATGTTTATCATAAAGACTACACAGGGCTGAAACGGACCGCTTCCAACATTGTGCCTGGAGGCGTGAAGAAGTCAAACGAAGTAGAAGAAGACCGTTTCCTCGCACATACAAGGGCCCATCAGAAGCAAGTGAAGCAAAAGGAGAAAAAAGTAACCCAGTAA
Reference Cai D, Wei X, Qiu Y, et al. High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase[J]. Journal of Applied Microbiology, 2016, 121(3): 704-712. [PubMed]
Miscellaneous -