SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0011061
| Accession Number | SPSED0011061 |
| Secreted Protein | Nattokinase |
| Uniprot Accession Number | Q548F3 |
| GenBank Accession Number | AAK54130.1 |
| Source Organism of the Secreted Protein | Bacillus subtilis natto | Nucleotide Sequence of the Secreted Protein | Length = 1146 ntGTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATCTTTACGATGGCGTTCAGCAACATGTCTGCGCAGGCTGCCGGAAAAAGCAGTACAGAAAAGAAATACATTGTCGGATTTAAGCAGACAATGAGTGCCATGAGTTCCGCCAAGAAAAAGGATGTTATTTCTGAAAAAGGCGGAAAGGTTCAAAAGCAATTTAAGTATGTTAACGCGGCCGCAGCAACATTGGATGAAAAAGCTGTAAAAGAATTGAAAAAAGATCCGAGCGTTGCATATGTGGAAGAAGATCATATTGCACATGAATATGCGCAATCTGTTCCTTATGGCATTTCTCAAATTAAAGCGCCGGCTCTTCACTCTCAAGGCTACACAGGCTCTAACGTAAAAGTAGCTGTTATCGACAGCGGAATTGACTCTTCTCATCCTGACTTAAACGTCAGAGGCGGAGCAAGCTTCGTTCCTTCTGAAACAAACCCATACCAGGACGGCAGTTCTCACGGTACGCATGTCGCCGGTACGATTGCCGCTCTTAATAACTCAATCGGTGTTCTGGGCGTAGCGCCAAGCGCATCATTATATGCAGTAAAAGTGCTTGATTCAACAGGAAGCGGCCAATATAGCTGGATTATTAACGGCATTGAGTGGGCCATTTCCAACAATATGGATGTTATCAACATGAGCCTTGGCGGACCTACTGGTTCTACAGCGCTGAAAACAGTAGTTGATAAAGCGGTTTCCAGCGGTATCGTCGTTGCTGCCGCAGCCGGAAACGAAGGTTCATCCGGAAGCACAAGCACAGTCGGCTACCCTGCAAAATATCCTTCTACTATTGCAGTAGGTGCGGTAAACAGCAGCAACCAAAGAGCTTCATTCTCCAGCGTAGGTTCTGAGCTTGATGTAATGGCTCCTGGCGTGTCCATCCAAAGCACACTTCCTGGAGGCACTTACGGCGCTTATAACGGAACGTCCATGGCGACTCCTCACGTTGCCGGAGCAGCAGCGCTAATTCTTTCTAAGCACCCGACTTGGACAAACGCGCAAGTCCGTGATCGTTTAGAAAGCACTGCAACATATCTTGGAAACTCTTTCTACTATGGAAAAGGGTTAATCAACGTACAAGCAGCTGCACAATAA |
| Amino Acid Sequence of the Secreted Protein | Length = 381 aaMRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ |
| Expression Host | Bacillus licheniformis △0F-3 |
| Source Organism of the Signal Peptide | Bacillus licheniformis WX-02 |
| Source Gene of the Signal Peptide | yvfO |
| Signal Peptide Sequence | Length = 26 aaMKNVLAVFVVLIFVLGAFGTSGPAEA |
| Type | Lip |
| Yield | 3.67 (FU/mL) |
| Performance (Current Yield / Highest Yield) | 0.11 |
| Charge | 0 |
| Charge (N-Region) | 1 |
| Hydrophobicity | 1.51 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 3.01 |
| Signal Peptide Original Protein Sequence | MKNVLAVFVVLIFVLGAFGTSGPAEAAARDSGTAKSDLYVEKVSGLRKDFIKGVDVSSIIALEESGVAFYNESGKKQDIFKTLKEAGVNYVRVRIWNDPYDANGNGYGGGNNDLEKAIQIGKRATANGMKLLADFHYSDFWADPAKQKAPKAWANLNFEDKKTALYQYTKQSLKAMKAAGIDIGMVQVGNETNGGLAGETDWAKMSQLFNAGSQAVRETDSNILVALHFTNPETSGRYAWIAETLHRHHVDYDVFASSYYPFWHGTLKNLTSVLTSVADTYGKKVMVAETSYTYTAEDGDGHGNTAPKNGQTLNYPVTVQGQANAVRDVIQAVSDVGEAGIGVFYWEPAWIPVGPAHRLEKNKALWETYGSGWATSYAAEYDPEDAGKWFGGSAVDNQALFDFKGRPLPSLHVFQYVDTGTPFKN |
| Signal Peptide Original DNA Sequence | TTGATGAAGAACGTGCTGGCTGTTTTTGTGGTGCTGATATTTGTTTTGGGCGCATTTGGAACAAGCGGTCCGGCGGAAGCCGCCAGAGATTCAGGGACGGCAAAAAGCGACCTTTATGTTGAAAAGGTTTCAGGGCTTCGCAAGGATTTTATAAAAGGGGTTGATGTTTCGAGCATCATCGCTTTGGAAGAGAGCGGCGTCGCCTTTTACAATGAATCCGGAAAGAAACAGGATATATTCAAAACGCTGAAGGAAGCAGGCGTCAATTATGTCCGGGTGCGCATATGGAATGATCCCTATGATGCCAACGGCAACGGCTACGGAGGGGGCAATAATGATCTGGAAAAAGCGATTCAGATCGGAAAACGGGCCACTGCCAACGGAATGAAGCTGCTGGCGGATTTCCACTATTCCGATTTCTGGGCGGACCCGGCGAAACAGAAAGCGCCGAAAGCATGGGCGAACCTAAACTTTGAAGATAAAAAGACGGCGCTTTATCAATATACAAAACAAAGCCTTAAAGCGATGAAAGCAGCGGGCATCGACATCGGCATGGTGCAGGTCGGTAACGAAACAAACGGGGGCCTTGCCGGTGAAACGGATTGGGCGAAGATGAGCCAATTATTCAACGCCGGGAGTCAAGCGGTGCGAGAGACGGATTCGAATATCCTAGTCGCCTTGCATTTTACCAATCCGGAGACGTCAGGAAGGTACGCTTGGATTGCCGAGACGCTTCATCGGCATCATGTAGACTACGATGTGTTTGCAAGCTCGTATTATCCGTTTTGGCATGGCACGCTGAAGAATTTAACATCCGTCCTGACATCTGTCGCAGATACGTACGGCAAAAAAGTCATGGTTGCTGAGACATCTTATACGTATACGGCTGAAGACGGAGACGGACACGGAAATACAGCTCCGAAAAACGGCCAAACGCTGAATTATCCGGTCACCGTTCAAGGGCAGGCGAACGCGGTCCGTGATGTGATTCAAGCGGTCAGCGACGTAGGTGAAGCCGGAATCGGCGTTTTCTATTGGGAACCGGCATGGATTCCGGTAGGACCGGCTCACCGGCTCGAGAAAAATAAAGCGCTTTGGGAGACATACGGATCAGGCTGGGCTACAAGCTATGCTGCTGAATATGACCCGGAAGACGCAGGAAAGTGGTTTGGCGGCAGCGCCGTAGACAATCAGGCATTGTTTGATTTTAAAGGACGTCCATTGCCGTCGCTTCATGTGTTTCAATATGTTGATACGGGAACACCATTCAAAAATTGA |
| Reference | Cai D, Wei X, Qiu Y, et al. High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase[J]. Journal of Applied Microbiology, 2016, 121(3): 704-712. [PubMed] |
| Miscellaneous | - |