SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0012031

Accession Number SPSED0012031
Secreted Protein Cutinase
Uniprot Accession Number P00590
GenBank Accession Number AAA33335.1
Source Organism of the Secreted Protein Fusarium solani pisi
Nucleotide Sequence of the Secreted Protein Length = 693 nt
ATGAAATTCTTCGCTCTCACCACACTTCTCGCCGCCACGGCTTCGGCTCTGCCTACTTCTAACCCTGCCCAGGAGCTTGAGGCGCGCCAGCTTGGTAGAACAACTCGCGACGATCTGATCAACGGCAATAGCGCTTCCTGCGCCGATGTCATCTTCATTTATGCCCGAGGTTCAACAGAGACGGGCAACTTGGGAACTCTCGGTCCTAGCATTGCCTCCAACCTTGAGTCCGCCTTCGGCAAGGACGGTGTCTGGATTCAGGGCGTTGGCGGTGCCTACGCAGCCACTCTTGGAGACAATGCTCTCCCTCGCGGAACCTCTAGCGCCGCAATCAGGGAGATGCTCGGTCTCTTCCAGCAGGCCAACACCAAGTGCCCTGACGCGACTTTGATCGCCGGTGGCTACAGCCAGGGTGCTGCACTTGCAGCCGCCTCCATCGAGGACCTCGACTCGGCCATTCGTGACAAGATCGCCGGAACTGTTCTGTTCGGCTACACCAAGAACCTACAGAACCGTGGCCGAATCCCCAACTACCCTGCCGACAGGACCAAGGTCTTCTGCAATACAGGGGATCTCGTTTGTACTGGTAGCTTGATCGTTGCTGCACCTCACTTGGCTTATGGTCCTGATGCTCGTGGCCCTGCCCCTGAGTTCCTCATCGAGAAGGTTCGGGCTGTCCGTGGTTCTGCTTGA
Amino Acid Sequence of the Secreted Protein Length = 230 aa
MKFFALTTLLAATASALPTSNPAQELEARQLGRTTRDDLINGNSASCRDVIFIYARGSTETGNLGTLGPSIASNLESAFGKDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNTGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA
Expression Host Corynebacterium glutamicum

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide ywmD
Signal Peptide Sequence Length = 23 aa
MKKLLAAGIIGLLTVSIASPSFA
Type Sec
Yield 4.5 (U/mL)
Performance (Current Yield / Highest Yield) 0.34
Charge 2
Charge (N-Region) 2
Hydrophobicity 1.37
Hydrophobicity Plot
Hydrophobicity (H-region) 2.4
Signal Peptide Original Protein Sequence MKKLLAAGIIGLLTVSIASPSFAAEKQADTNVAVLFDGSGSMVQKTGGERKIDIAKKSVKSFAELLPKDTNLMLRVFGHAGNNKLSGKALSCSTTETIYGLHPYEGSLFDNSLSELKPTGWTPIAKALADTRKEFEAFDADGKNVVYLITDGEETCGGDPAAEIEKLRASNVDTIVNIIGFNFDVKGNEEMKQAAVAGGGEYISANSADEFEQAWEKEAQKFTE
Signal Peptide Original DNA Sequence ATGAAAAAATTGCTGGCTGCCGGTATCATTGGATTGTTGACTGTTTCCATTGCCTCCCCGTCTTTTGCCGCTGAGAAACAGGCTGATACAAATGTAGCTGTTTTGTTTGACGGAAGCGGAAGCATGGTTCAAAAAACAGGAGGAGAGCGCAAAATAGACATTGCCAAAAAATCAGTAAAATCTTTTGCCGAACTTCTTCCAAAGGATACAAATCTTATGCTTCGCGTTTTCGGGCATGCAGGGAACAATAAACTGTCCGGTAAAGCCCTTTCGTGCAGTACGACGGAAACCATTTACGGCCTTCATCCATATGAAGGGTCTCTGTTTGACAATTCATTGAGTGAGCTCAAGCCGACTGGATGGACACCGATTGCCAAAGCACTTGCAGACACAAGAAAAGAATTTGAAGCATTTGACGCAGACGGGAAAAACGTGGTGTATTTAATCACTGACGGTGAAGAAACGTGCGGCGGAGACCCTGCGGCGGAAATCGAAAAGCTCCGTGCATCAAATGTGGATACGATTGTAAACATCATTGGCTTCAACTTTGATGTCAAGGGTAATGAAGAGATGAAACAAGCAGCTGTCGCTGGCGGCGGAGAATATATTTCAGCGAACAGTGCGGATGAGTTCGAACAGGCTTGGGAAAAAGAAGCACAAAAATTTACTGAGTAA
Reference Hemmerich J, Rohe P, Kleine B, et al. Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum[J]. Microbial Cell Factories, 2016, 15(1): 208. [PubMed]
Miscellaneous -