Accession Number |
SPSED0012052 |
Secreted Protein |
Cutinase |
Uniprot Accession Number |
P00590 |
GenBank Accession Number |
AAA33335.1 |
Source Organism of the Secreted Protein |
Fusarium solani pisi |
Nucleotide Sequence of the Secreted Protein |
Length = 693 ntATGAAATTCTTCGCTCTCACCACACTTCTCGCCGCCACGGCTTCGGCTCTGCCTACTTCTAACCCTGCCCAGGAGCTTGAGGCGCGCCAGCTTGGTAGAACAACTCGCGACGATCTGATCAACGGCAATAGCGCTTCCTGCGCCGATGTCATCTTCATTTATGCCCGAGGTTCAACAGAGACGGGCAACTTGGGAACTCTCGGTCCTAGCATTGCCTCCAACCTTGAGTCCGCCTTCGGCAAGGACGGTGTCTGGATTCAGGGCGTTGGCGGTGCCTACGCAGCCACTCTTGGAGACAATGCTCTCCCTCGCGGAACCTCTAGCGCCGCAATCAGGGAGATGCTCGGTCTCTTCCAGCAGGCCAACACCAAGTGCCCTGACGCGACTTTGATCGCCGGTGGCTACAGCCAGGGTGCTGCACTTGCAGCCGCCTCCATCGAGGACCTCGACTCGGCCATTCGTGACAAGATCGCCGGAACTGTTCTGTTCGGCTACACCAAGAACCTACAGAACCGTGGCCGAATCCCCAACTACCCTGCCGACAGGACCAAGGTCTTCTGCAATACAGGGGATCTCGTTTGTACTGGTAGCTTGATCGTTGCTGCACCTCACTTGGCTTATGGTCCTGATGCTCGTGGCCCTGCCCCTGAGTTCCTCATCGAGAAGGTTCGGGCTGTCCGTGGTTCTGCTTGA |
Amino Acid Sequence of the Secreted Protein |
Length = 230 aaMKFFALTTLLAATASALPTSNPAQELEARQLGRTTRDDLINGNSASCRDVIFIYARGSTETGNLGTLGPSIASNLESAFGKDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNTGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA |
Expression Host |
Corynebacterium glutamicum |
|
Source Organism of the Signal Peptide |
Bacillus subtilis |
Source Gene of the Signal Peptide |
amiE |
Signal Peptide Sequence |
Length = 25 aaMKTKTLFIFSAILTLSIFAPNETFA |
Type |
Sec |
Yield |
0.9 (U/mL) |
Performance (Current Yield / Highest Yield) |
0.07 |
Charge |
1 |
Charge (N-Region) |
2 |
Hydrophobicity |
0.9 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
2.26 |
Signal Peptide Original Protein Sequence |
MKTKTLFIFSAILTLSIFAPNETFAQTAGNLIEPKIINAETAQFSTKKLRKVDQMIERDIAAGFPGAVLVVVKDGRIIKKAAYGYSKKYEGSELLRRPAKMKTRTMFDLASNTKMYATNFALQRLVSQGKLDVYEKVSAYLPGFKDQPGDLIKGKDKIRVIDVLQHQSGLPSSFYFYTPEKAGKYYSQERDKTIEYLTKIPLDYQTGTKHVYSDIGYMLLGCIVEKLTGKPLDVYTEQELYKPLRLKHTLYNPLQKGFKPKQFAATERMGNTRDGVIQFPNIRTNTLQGEVHDEKAFYSMDGVSGHAGLFSNADDMAILLQVMLNKGSYRNISLFDQKTADLFTAPSATDPTFALGWRRNGSKSMEWMFGPHASENAYGHTGWTGTVTIIDPAYNLGIALLTNKKHTPVIDPEENPNVFEGDQFPTGSYGSVITAIYEAME |
Signal Peptide Original DNA Sequence |
GTGAAAACAAAGACACTGTTCATATTCAGTGCCATTTTAACCCTATCGATATTTGCGCCAAATGAAACCTTCGCACAAACAGCAGGCAACTTGATTGAGCCGAAAATCATAAATGCAGAAACTGCACAATTCTCGACGAAGAAGCTAAGGAAGGTAGATCAGATGATAGAACGGGATATTGCAGCAGGATTTCCAGGCGCCGTTCTTGTGGTGGTGAAGGATGGACGCATCATAAAAAAAGCAGCATACGGCTACAGTAAAAAGTACGAAGGATCAGAACTGCTGCGCCGTCCGGCCAAAATGAAAACCCGCACGATGTTTGACTTAGCCTCAAATACAAAAATGTACGCAACAAATTTTGCATTGCAGCGCTTAGTCAGCCAAGGAAAACTTGACGTCTATGAAAAAGTTTCTGCATATTTGCCGGGTTTCAAGGATCAGCCGGGTGACCTCATAAAAGGAAAAGACAAGATACGTGTCATTGATGTCCTCCAGCACCAATCAGGGCTCCCATCAAGCTTTTACTTTTACACGCCGGAAAAAGCCGGCAAGTACTATTCCCAAGAACGGGACAAAACGATTGAATATTTGACGAAAATTCCGCTCGATTATCAAACCGGGACAAAACACGTATACAGTGACATTGGATATATGCTACTCGGATGTATTGTCGAAAAACTCACCGGCAAACCGCTTGATGTCTACACGGAGCAAGAGCTCTACAAACCGCTCAGGCTGAAGCATACCTTGTACAATCCGCTGCAAAAGGGCTTTAAACCGAAACAGTTCGCAGCTACTGAACGCATGGGCAATACACGTGACGGCGTTATTCAATTCCCTAACATCCGCACAAACACGCTGCAAGGAGAGGTGCATGACGAAAAAGCGTTTTACTCAATGGACGGCGTGTCTGGGCATGCCGGATTGTTCTCAAATGCAGATGATATGGCCATACTGCTGCAAGTCATGTTAAACAAAGGTTCATACCGAAACATTTCTTTATTTGATCAGAAAACAGCCGACCTGTTTACAGCACCTTCAGCAACAGATCCGACATTCGCTCTTGGCTGGAGGCGGAACGGAAGTAAAAGCATGGAATGGATGTTTGGCCCTCATGCCAGCGAAAATGCTTACGGGCATACCGGCTGGACGGGGACAGTTACAATTATTGATCCAGCATACAATTTAGGCATCGCATTGTTAACAAATAAAAAGCATACACCAGTTATTGATCCGGAAGAAAATCCGAACGTCTTTGAAGGTGACCAATTTCCAACAGGCAGTTATGGAAGTGTCATAACCGCGATTTATGAAGCGATGGAGTGA |
Reference |
Hemmerich J, Rohe P, Kleine B, et al. Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum[J]. Microbial Cell Factories, 2016, 15(1): 208. [PubMed] |
Miscellaneous |
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