Detail Information for SPSED0013051

Accession Number SPSED0013051
Secreted Protein Alkaline active xylanase (XynBYG) (promoter:Pglvm)
Uniprot Accession Number A0A172WBP7
GenBank Accession Number ANF06879.1
Source Organism of the Secreted Protein Bacillus pumilus BYG
Nucleotide Sequence of the Secreted Protein Length = 687 nt
ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA
Amino Acid Sequence of the Secreted Protein Length = 228 aa
MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN
Expression Host Bacillus subtilis WB700

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide amiE
Signal Peptide Sequence Length = 25 aa
MKTKTLFIFSAILTLSIFAPNETFA
Type Sec
Yield 109.4 (U/mL)
Performance (Current Yield / Highest Yield) 0.33
Charge 1
Charge (N-Region) 2
Hydrophobicity 0.9
Hydrophobicity Plot
Hydrophobicity (H-region) 2.26
Signal Peptide Original Protein Sequence MKTKTLFIFSAILTLSIFAPNETFAQTAGNLIEPKIINAETAQFSTKKLRKVDQMIERDIAAGFPGAVLVVVKDGRIIKKAAYGYSKKYEGSELLRRPAKMKTRTMFDLASNTKMYATNFALQRLVSQGKLDVYEKVSAYLPGFKDQPGDLIKGKDKIRVIDVLQHQSGLPSSFYFYTPEKAGKYYSQERDKTIEYLTKIPLDYQTGTKHVYSDIGYMLLGCIVEKLTGKPLDVYTEQELYKPLRLKHTLYNPLQKGFKPKQFAATERMGNTRDGVIQFPNIRTNTLQGEVHDEKAFYSMDGVSGHAGLFSNADDMAILLQVMLNKGSYRNISLFDQKTADLFTAPSATDPTFALGWRRNGSKSMEWMFGPHASENAYGHTGWTGTVTIIDPAYNLGIALLTNKKHTPVIDPEENPNVFEGDQFPTGSYGSVITAIYEAME
Signal Peptide Original DNA Sequence GTGAAAACAAAGACACTGTTCATATTCAGTGCCATTTTAACCCTATCGATATTTGCGCCAAATGAAACCTTCGCACAAACAGCAGGCAACTTGATTGAGCCGAAAATCATAAATGCAGAAACTGCACAATTCTCGACGAAGAAGCTAAGGAAGGTAGATCAGATGATAGAACGGGATATTGCAGCAGGATTTCCAGGCGCCGTTCTTGTGGTGGTGAAGGATGGACGCATCATAAAAAAAGCAGCATACGGCTACAGTAAAAAGTACGAAGGATCAGAACTGCTGCGCCGTCCGGCCAAAATGAAAACCCGCACGATGTTTGACTTAGCCTCAAATACAAAAATGTACGCAACAAATTTTGCATTGCAGCGCTTAGTCAGCCAAGGAAAACTTGACGTCTATGAAAAAGTTTCTGCATATTTGCCGGGTTTCAAGGATCAGCCGGGTGACCTCATAAAAGGAAAAGACAAGATACGTGTCATTGATGTCCTCCAGCACCAATCAGGGCTCCCATCAAGCTTTTACTTTTACACGCCGGAAAAAGCCGGCAAGTACTATTCCCAAGAACGGGACAAAACGATTGAATATTTGACGAAAATTCCGCTCGATTATCAAACCGGGACAAAACACGTATACAGTGACATTGGATATATGCTACTCGGATGTATTGTCGAAAAACTCACCGGCAAACCGCTTGATGTCTACACGGAGCAAGAGCTCTACAAACCGCTCAGGCTGAAGCATACCTTGTACAATCCGCTGCAAAAGGGCTTTAAACCGAAACAGTTCGCAGCTACTGAACGCATGGGCAATACACGTGACGGCGTTATTCAATTCCCTAACATCCGCACAAACACGCTGCAAGGAGAGGTGCATGACGAAAAAGCGTTTTACTCAATGGACGGCGTGTCTGGGCATGCCGGATTGTTCTCAAATGCAGATGATATGGCCATACTGCTGCAAGTCATGTTAAACAAAGGTTCATACCGAAACATTTCTTTATTTGATCAGAAAACAGCCGACCTGTTTACAGCACCTTCAGCAACAGATCCGACATTCGCTCTTGGCTGGAGGCGGAACGGAAGTAAAAGCATGGAATGGATGTTTGGCCCTCATGCCAGCGAAAATGCTTACGGGCATACCGGCTGGACGGGGACAGTTACAATTATTGATCCAGCATACAATTTAGGCATCGCATTGTTAACAAATAAAAAGCATACACCAGTTATTGATCCGGAAGAAAATCCGAACGTCTTTGAAGGTGACCAATTTCCAACAGGCAGTTATGGAAGTGTCATAACCGCGATTTATGAAGCGATGGAGTGA
Reference Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed]
Miscellaneous promoter:Pglvm