Detail Information for SPSED0013064

Accession Number SPSED0013064
Secreted Protein Alkaline active xylanase (XynBYG) (promoter:Pglvm)
Uniprot Accession Number A0A172WBP7
GenBank Accession Number ANF06879.1
Source Organism of the Secreted Protein Bacillus pumilus BYG
Nucleotide Sequence of the Secreted Protein Length = 687 nt
ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA
Amino Acid Sequence of the Secreted Protein Length = 228 aa
MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN
Expression Host Bacillus subtilis WB700

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide yobB
Signal Peptide Sequence Length = 25 aa
MKIRKILLSSALSFGMLISAVPALA
Type Sec
Yield 89.7 (U/mL)
Performance (Current Yield / Highest Yield) 0.27
Charge 3
Charge (N-Region) 3
Hydrophobicity 1.32
Hydrophobicity Plot
Hydrophobicity (H-region) 1.85
Signal Peptide Original Protein Sequence MKIRKILLSSALSFGMLISAVPALAAGTSSEVAVKNEEYDYDTIYRISPLPMDFQPTIEYNGYTYTLTRHYFDYSIGFYTAIYTKVV
Signal Peptide Original DNA Sequence TTGAAGATTAGGAAAATCTTATTGAGTTCTGCTTTATCCTTTGGCATGCTAATATCCGCGGTTCCCGCATTGGCTGCTGGCACTTCGTCTGAGGTTGCAGTAAAAAATGAAGAATATGATTATGACACTATATACCGCATTAGTCCTCTGCCAATGGACTTCCAGCCAACAATAGAATACAACGGTTATACTTATACGCTTACACGTCATTATTTTGATTACTCAATCGGGTTTTACACGGCTATTTATACAAAAGTTGTTTAA
Reference Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed]
Miscellaneous promoter:Pglvm