Detail Information for SPSED0013066

Accession Number SPSED0013066
Secreted Protein Alkaline active xylanase (XynBYG) (promoter:Pglvm)
Uniprot Accession Number A0A172WBP7
GenBank Accession Number ANF06879.1
Source Organism of the Secreted Protein Bacillus pumilus BYG
Nucleotide Sequence of the Secreted Protein Length = 687 nt
ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA
Amino Acid Sequence of the Secreted Protein Length = 228 aa
MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN
Expression Host Bacillus subtilis WB700

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide dacC
Signal Peptide Sequence Length = 28 aa
KKSIKLYVAVLLLFVVASVPYMHQAALA
Type Sec
Yield 88.1 (U/mL)
Performance (Current Yield / Highest Yield) 0.27
Charge 3
Charge (N-Region) 3
Hydrophobicity 1.21
Hydrophobicity Plot
Hydrophobicity (H-region) 2.59
Signal Peptide Original Protein Sequence KKSIKLYVAVLLLFVVASVPYMHQAALAAAEKQDALSGQIDKILADHPALEGAMAGITVRSAETGAVLYEHSGDTRMRPASSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTWHDDMRLSPDMPWSDEYTYYGAPISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKDLTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTGEAPSSSDVLLSHRSMPLSKLFVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNSTLPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQSWFSAYLNSLPVAGNPDRMVGGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDIEDQIAVILANQ
Signal Peptide Original DNA Sequence ATGAAAAAAAGCATAAAGCTTTATGTTGCTGTTTTACTGCTTTTCGTCGTTGCTTCGGTGCCTTATATGCATCAGGCTGCGCTTGCCGCTGAAAAACAAGATGCACTTTCCGGGCAGATCGATAAGATTTTAGCTGATCATCCTGCCCTCGAAGGCGCAATGGCCGGCATCACCGTTCGCTCTGCGGAAACAGGTGCTGTTCTATATGAACATTCAGGAGACACCCGAATGAGGCCTGCCTCTTCTCTAAAGCTTTTGACAGCGGCCGCGGCACTTTCTGTCCTTGGCGAGAACTATTCTTTTACAACCGAGGTCCGGACGGATGGAACACTGAAAGGGAAAAAGCTGAATGGTAACCTTTATTTGAAAGGGAAAGGCGATCCGACACTTTTACCATCTGATTTTGACAAAATGGCTGAGATATTGAAACATTCCGGCGTAAAAGTAATTAAAGGCAATCTGATCGGTGATGATACGTGGCATGATGACATGCGATTATCACCTGATATGCCTTGGAGCGATGAATACACATATTATGGCGCGCCAATCTCCGCTCTAACAGCGTCTCCAAACGAAGACTATGATGCAGGCACTGTGATTGTTGAGGTCACCCCAAATCAAAAAGAAGGGGAAGAGCCTGCCGTGTCTGTCTCTCCGAAAACCGATTATATAACGATAAAGAACGATGCTAAAACGACTGCTGCCGGTTCAGAGAAAGACCTCACAATCGAAAGAGAGCATGGAACAAACACGATTACAATTGAAGGGAGCGTACCGGTTGACGCAAACAAGACAAAGGAATGGATTTCTGTCTGGGAGCCTGCCGGATATGCCTTAGATTTATTTAAGCAGTCACTAAAAAAACAAGGTATTACTGTGAAAGGGGACATCAAGACGGGTGAAGCACCTAGTTCTTCAGATGTGCTGCTCTCTCATCGCTCAATGCCTTTATCGAAACTTTTTGTGCCGTTCATGAAATTAAGTAATAACGGACATGCCGAAGTGCTCGTAAAAGAGATGGGGAAAGTGAAAAAAGGAGAAGGGAGCTGGGAAAAGGGGCTCGAAGTGTTAAACAGTACGCTTCCGGAATTTGGTGTTGATTCTAAATCACTCGTCTTAAGGGACGGATCAGGAATCTCACATATTGATGCTGTATCCTCAGATCAACTTTCACAGCTGTTATATGACATTCAGGATCAGAGTTGGTTCTCGGCTTATCTAAATTCTTTACCTGTTGCGGGGAATCCTGACAGAATGGTGGGCGGAACGCTGAGAAACCGCATGAAAGGCACTCCTGCACAAGGAAAAGTAAGAGCAAAAACCGGATCGCTCAGTACGGTAAGCTCTCTATCGGGGTACGCAGAAACAAAGAGCGGAAAAAAACTTGTTTTCTCTATTCTTCTAAATGGATTGATTGATGAAGAGGATGGAAAAGATATTGAGGATCAAATCGCTGTCATTTTGGCAAATCAATAA
Reference Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed]
Miscellaneous promoter:Pglvm