Accession Number | SPSED0013108 |
Secreted Protein | Alkaline active xylanase (XynBYG) (promoter:Pglvm) |
Uniprot Accession Number | A0A172WBP7 |
GenBank Accession Number | ANF06879.1 |
Source Organism of the Secreted Protein | Bacillus pumilus BYG |
Nucleotide Sequence of the Secreted Protein | Length = 687 nt ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA |
Amino Acid Sequence of the Secreted Protein | Length = 228 aa MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN |
Expression Host | Bacillus subtilis WB700 |
Source Organism of the Signal Peptide | Bacillus subtilis |
Source Gene of the Signal Peptide | skiX |
Signal Peptide Sequence | Length = 33 aa MKKVWIGIGIAVIVALFVGINIYRSAAPTSGSA |
Type | Sec |
Yield | 3.1 (U/mL) |
Performance (Current Yield / Highest Yield) | 0.01 |
Charge | 3 |
Charge (N-Region) | 2 |
Hydrophobicity | 1.12 |
Hydrophobicity Plot | |
Hydrophobicity (H-region) | 2.44 |
Signal Peptide Original Protein Sequence | MKKVWIGIGIAVIVALFVGINIYRSAAPTSGSAGKEVQTGSVEENEISSTVMVPGTLKFSNEQYVFYEADKGTLEDIKVKEGDKVKKGTALVTYTNEQLSLEKEQNQLTSESNRLQIDQIQEKLKALDSKERELEKQVGKKEAEKQIESERTELQMQKKTAEIELKQTELQRQSLANRVSDLEVKSEIEGTVISVNQEAASKKSDIQEPVIHIGNPKDLVVSGKLSEYDTLKVKKGQKVTLTSDVIQGKTWKGTVSAVGLVPDQQESAAAQGTEQAVQYPLQVKIKGNLPEGKPGFKFIMNIETDKRKANTLPSKAVKKEDDQYYVYTVKDGKAKRVDVKIGEVTDDLTEIKEGLTQDDQVILNPSDQVTDGMEVKS |
Signal Peptide Original DNA Sequence | ATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATGAACAGTATGTCTTTTATGAAGCGGATAAAGGGACATTAGAAGACATTAAAGTAAAGGAAGGCGATAAAGTTAAAAAGGGCACGGCTTTAGTCACCTATACAAATGAGCAGCTGAGCCTGGAAAAAGAACAGAACCAGTTAACGTCTGAATCCAACCGGCTTCAAATTGACCAAATTCAAGAAAAGTTAAAAGCCTTGGACAGCAAAGAAAGAGAACTGGAAAAACAGGTAGGAAAGAAAGAAGCAGAAAAACAAATCGAATCTGAACGGACAGAGCTTCAAATGCAGAAAAAAACAGCGGAGATCGAATTAAAACAAACCGAGCTTCAGCGGCAGTCACTTGCCAACCGGGTGTCAGATTTAGAAGTGAAAAGCGAAATTGAAGGCACCGTTATTTCTGTCAACCAAGAAGCGGCATCCAAAAAGTCAGATATTCAAGAGCCTGTCATACATATCGGCAACCCAAAAGACCTTGTCGTCTCAGGGAAATTGTCTGAATACGATACATTGAAAGTCAAAAAAGGCCAGAAGGTCACACTCACTTCAGATGTAATACAGGGTAAAACATGGAAAGGCACGGTTTCCGCAGTCGGGCTTGTTCCGGATCAGCAGGAGAGCGCTGCGGCACAAGGAACAGAACAGGCGGTCCAATACCCGCTTCAAGTGAAAATAAAAGGAAATCTTCCAGAAGGAAAGCCAGGCTTTAAATTTATCATGAATATTGAAACAGATAAACGGAAAGCAAATACGCTTCCTTCAAAGGCAGTCAAAAAAGAAGATGACCAATATTATGTGTATACAGTAAAAGACGGAAAAGCAAAGCGAGTTGATGTCAAAATCGGCGAAGTGACAGATGACCTGACAGAAATTAAAGAGGGGCTTACCCAAGATGATCAAGTCATTTTGAATCCATCTGATCAGGTGACTGACGGAATGGAAGTGAAATCCTAA |
Reference | Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
Miscellaneous | promoter:Pglvm |