Accession Number |
SPSED0014008 |
Secreted Protein |
Alkaline active xylanase (XynBYG) (promoter:P43) |
Uniprot Accession Number |
A0A172WBP7 |
GenBank Accession Number |
ANF06879.1 |
Source Organism of the Secreted Protein |
Bacillus pumilus BYG |
Nucleotide Sequence of the Secreted Protein |
Length = 687 ntATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA |
Amino Acid Sequence of the Secreted Protein |
Length = 228 aaMNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN |
Expression Host |
Bacillus subtilis WB700 |
|
Source Organism of the Signal Peptide |
Bacillus subtilis |
Source Gene of the Signal Peptide |
yndA |
Signal Peptide Sequence |
Length = 25 aaMRFTKVVGFLSVLGLAAVFPLTAQA |
Type |
Sec |
Yield |
144.2 (U/mL) |
Performance (Current Yield / Highest Yield) |
0.74 |
Charge |
2 |
Charge (N-Region) |
2 |
Hydrophobicity |
1.32 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
2.19 |
Signal Peptide Original Protein Sequence |
MRFTKVVGFLSVLGLAAVFPLTAQAEKAGTAGAGEWDKLGTYTYTYSSPTVYSTGGDFRVCLSGSTPFSVSLHLYEDDPGDNPDDYVGANYFSPGECHTFGSIGKFVDGSNNKAEFFVTDYSGKSKTVTFYD |
Signal Peptide Original DNA Sequence |
ATGAGATTCACTAAGGTAGTTGGATTTTTGTCTGTTTTAGGGTTGGCTGCGGTTTTTCCATTAACGGCACAAGCAGAAAAAGCCGGAACTGCGGGGGCTGGAGAGTGGGATAAGTTGGGGACTTACACATATACTTACTCAAGTCCGACGGTATACTCGACCGGAGGAGATTTTAGAGTGTGCCTTTCAGGAAGCACTCCTTTTTCTGTTTCACTGCACTTATATGAGGATGATCCCGGTGACAATCCTGATGATTATGTAGGGGCAAACTATTTTTCACCCGGAGAATGCCACACCTTTGGCAGTATTGGAAAATTTGTAGATGGGAGCAATAACAAGGCCGAGTTCTTTGTTACCGATTACTCTGGTAAATCTAAAACTGTTACGTTTTATGATTGA |
Reference |
Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
Miscellaneous |
promoter:P43 |