SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0014033

Accession Number SPSED0014033
Secreted Protein Alkaline active xylanase (XynBYG) (promoter：P43)
Uniprot Accession Number A0A172WBP7
GenBank Accession Number ANF06879.1
Source Organism of the Secreted Protein Bacillus pumilus BYG
Nucleotide Sequence of the Secreted Protein Length = 687 nt
ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA
Amino Acid Sequence of the Secreted Protein Length = 228 aa
MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN
Expression Host Bacillus subtilis WB700

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide tlpA
Signal Peptide Sequence Length = 39 aa
MKKTLTTIRRSSIARRLIISFLLILIVPITALSVSAYQS
Type Tat
Yield 84 (U/mL)
Performance (Current Yield / Highest Yield) 0.43
Charge 6
Charge (N-Region) 6
Hydrophobicity 0.85
Hydrophobicity Plot
Hydrophobicity (H-region) 2.62
Signal Peptide Original Protein Sequence MKKTLTTIRRSSIARRLIISFLLILIVPITALSVSAYQSAVASLDVQMTQSAKENVQILDHIIDDKISTTEKSLAYFSDWATAEKFQDKKKTELKQEFKQFIQMNDNVAAVFSSGKDGDFTRYPYADMPSDFNALERDWYKEAMANKGKTIVTEPYESISSGKMVVTIARQTVDGSGVVAIDMKIDDLVTTAKGINIGKEGYAFILSQNKKVIAYSGEKAGTELKGDWVDKLYKDKSGDFEYTYKGKKKKMAFATSQTTGWKISGTMYANEIHDAASRVLIMASIVLAIAIGAGMTAIYFVIRSITKPLRRIVASAEKISEGDLTETIEINSKDELGVLSESFNHMAHSLRSLIHGIKDSVEHVASSSEELTASADQTSRATEHITMAIEQFSNGSESQSEKIETTTEQINEMNDGLAELARAAAVITETSADSTEVSSKGETLVQKTAGQMNTIDHSVKAAEQVVKGLEIKSKDITNILRVINGIADQTNLLALNAAIEAARAGEYGRGFSVVAEEVRKLAVQSADSAKEIESLISEIVKEIHTSLNVLQSVNKEVETGLVMTDETKQSFKHISQMTNQIASELQNMNATVEELSAGAQEISAASNDITAISKESSDGIQDIAASAEEQLASMEEISSSALTLERMSEELRDLTKQFKVDK
Signal Peptide Original DNA Sequence ATGAAAAAAACACTCACCACTATTCGCAGATCATCAATTGCAAGGAGACTTATTATTTCTTTCCTGCTGATCTTAATTGTTCCGATAACCGCCCTTTCGGTTAGCGCTTATCAATCAGCAGTTGCCTCACTTGATGTACAGATGACGCAAAGCGCAAAGGAAAATGTTCAGATATTAGATCACATCATTGATGATAAAATCAGCACTACTGAAAAAAGCCTCGCATATTTCAGCGATTGGGCTACAGCAGAAAAATTTCAGGATAAAAAGAAAACAGAGCTGAAACAGGAATTTAAACAATTTATCCAAATGAATGATAATGTCGCTGCGGTATTTTCAAGCGGCAAAGACGGGGACTTCACACGTTATCCGTACGCTGACATGCCAAGCGACTTTAATGCTTTGGAACGAGATTGGTACAAAGAAGCAATGGCCAATAAAGGCAAAACAATTGTCACTGAGCCTTATGAATCGATTTCATCAGGAAAAATGGTCGTCACCATCGCCCGTCAAACGGTGGATGGGTCAGGCGTTGTCGCCATTGATATGAAAATTGATGACTTAGTCACTACCGCGAAAGGAATCAATATCGGAAAAGAAGGCTATGCTTTTATTTTGAGCCAAAACAAGAAAGTCATCGCCTACTCCGGAGAAAAAGCGGGAACAGAGCTAAAAGGGGACTGGGTTGATAAGCTCTACAAGGATAAAAGCGGGGACTTTGAATATACGTACAAAGGCAAAAAGAAAAAAATGGCATTTGCAACAAGCCAAACAACAGGCTGGAAAATCAGCGGCACGATGTATGCAAACGAGATTCACGATGCTGCAAGCAGGGTTCTGATCATGGCTTCAATCGTTCTGGCGATTGCCATCGGAGCTGGAATGACGGCGATTTATTTTGTGATTCGATCCATCACAAAGCCATTGAGACGCATTGTCGCATCCGCTGAAAAAATTAGTGAAGGCGATCTAACCGAAACGATTGAAATCAATTCAAAAGATGAACTCGGCGTTCTGAGTGAGAGCTTCAATCATATGGCACACTCGCTTCGCTCGCTCATTCACGGGATCAAAGACTCAGTAGAACACGTTGCCTCCTCGTCTGAAGAGCTGACTGCATCGGCAGACCAGACAAGCAGAGCCACTGAGCACATTACGATGGCTATCGAGCAATTTTCAAACGGCAGTGAAAGCCAAAGCGAGAAAATAGAAACGACAACCGAACAAATTAATGAAATGAATGACGGATTGGCAGAGCTGGCGCGGGCTGCAGCAGTGATTACAGAAACATCCGCAGATTCAACCGAAGTGTCAAGCAAAGGGGAGACACTCGTCCAGAAAACAGCCGGCCAAATGAACACAATTGACCATTCCGTAAAAGCAGCGGAACAAGTGGTGAAAGGGCTGGAGATCAAATCAAAGGATATCACGAATATTTTGCGTGTCATCAACGGCATTGCGGATCAAACCAATCTTTTGGCTTTAAATGCCGCCATTGAAGCTGCCAGAGCGGGTGAATACGGCCGGGGCTTCTCAGTCGTCGCTGAGGAAGTGAGAAAGCTCGCGGTACAGTCGGCTGACTCAGCAAAAGAAATTGAATCGCTGATCAGTGAAATAGTAAAAGAAATTCACACATCACTGAACGTGCTTCAGTCCGTAAACAAAGAAGTTGAAACAGGGCTTGTCATGACGGATGAAACTAAACAGAGCTTCAAACATATATCGCAAATGACCAATCAAATTGCTTCCGAGCTGCAAAACATGAATGCGACGGTTGAGGAGCTGTCAGCGGGAGCTCAAGAAATTTCCGCAGCTTCAAATGATATCACTGCCATTTCTAAGGAAAGCTCGGATGGCATCCAAGATATCGCCGCTTCAGCTGAGGAACAGCTGGCATCCATGGAGGAGATTAGTTCATCTGCACTGACGCTTGAAAGAATGTCTGAAGAACTCCGTGATCTGACAAAACAATTTAAAGTAGACAAATAA
Reference Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed]
Miscellaneous promoter：P43