Accession Number | SPSED0014035 |
Secreted Protein | Alkaline active xylanase (XynBYG) (promoter:P43) |
Uniprot Accession Number | A0A172WBP7 |
GenBank Accession Number | ANF06879.1 |
Source Organism of the Secreted Protein | Bacillus pumilus BYG |
Nucleotide Sequence of the Secreted Protein | Length = 687 nt ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA |
Amino Acid Sequence of the Secreted Protein | Length = 228 aa MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN |
Expression Host | Bacillus subtilis WB700 |
Source Organism of the Signal Peptide | Bacillus subtilis |
Source Gene of the Signal Peptide | YqgA |
Signal Peptide Sequence | Length = 28 aa MKQGKFSVFLILLLMLTLVVAPKGKAEA |
Type | Sec |
Yield | 82 (U/mL) |
Performance (Current Yield / Highest Yield) | 0.42 |
Charge | 3 |
Charge (N-Region) | 2 |
Hydrophobicity | 1.01 |
Hydrophobicity Plot | |
Hydrophobicity (H-region) | 2.98 |
Signal Peptide Original Protein Sequence | - |
Signal Peptide Original DNA Sequence | - |
Reference | Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
Miscellaneous | promoter:P43 |