SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0014061

Accession Number SPSED0014061
Secreted Protein Alkaline active xylanase (XynBYG) (promoter：P43)
Uniprot Accession Number A0A172WBP7
GenBank Accession Number ANF06879.1
Source Organism of the Secreted Protein Bacillus pumilus BYG
Nucleotide Sequence of the Secreted Protein Length = 687 nt
ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA
Amino Acid Sequence of the Secreted Protein Length = 228 aa
MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN
Expression Host Bacillus subtilis WB700

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide ykwD
Signal Peptide Sequence Length = 26 aa
MKKAFILSAAAAVGLFTFGGVQQASA
Type Sec
Yield 51.4 (U/mL)
Performance (Current Yield / Highest Yield) 0.27
Charge 2
Charge (N-Region) 2
Hydrophobicity 0.97
Hydrophobicity Plot
Hydrophobicity (H-region) 1.96
Signal Peptide Original Protein Sequence MKKAFILSAAAAVGLFTFGGVQQASAKELSCQPVVTVKTGNTVQNMSLNDAVKKLHINTNIKTLNAANEKEMKQLLQKHAKQSNVKVQDVQKTETAKPAQKTTEKAAADQNTASKAPATAEKTNTTTSAPSSVSAYEKKVVELTNAERQKQGLKPLQIDETLSKSARAKSQDMKDKNYFDHQSPTYGSPFDMMKSFGISYKTAGENIAKGQKTPEEVVKAWMNSEGHRKNILNPNFTHIGVGYVESGSIWTQQFIGK
Signal Peptide Original DNA Sequence ATGAAGAAAGCATTTATTTTATCTGCTGCCGCTGCGGTTGGATTATTCACATTCGGGGGCGTACAGCAAGCATCAGCGAAAGAACTCTCCTGCCAGCCTGTGGTTACAGTAAAAACGGGGAACACAGTACAAAACATGTCACTTAACGATGCAGTGAAAAAACTGCATATTAACACAAATATCAAAACGTTAAACGCTGCAAACGAAAAAGAGATGAAGCAGCTTCTTCAAAAACATGCGAAGCAATCTAACGTGAAAGTTCAGGATGTTCAAAAGACAGAAACGGCAAAACCTGCACAAAAGACTACTGAAAAAGCAGCTGCTGATCAAAACACTGCTTCTAAAGCACCCGCAACTGCTGAAAAAACAAACACAACAACGTCTGCACCTTCATCTGTAAGTGCATATGAGAAAAAAGTTGTTGAACTTACAAATGCAGAAAGACAAAAACAAGGCTTAAAACCGCTTCAAATTGATGAAACGTTAAGCAAATCTGCACGCGCAAAATCTCAGGATATGAAAGACAAAAACTACTTTGATCACCAAAGCCCAACATACGGCTCACCGTTTGATATGATGAAATCTTTCGGAATCAGCTACAAAACAGCAGGTGAAAACATTGCGAAGGGCCAAAAGACACCAGAAGAAGTAGTAAAAGCTTGGATGAACAGTGAAGGACACAGAAAAAACATTCTAAACCCTAACTTTACTCACATCGGTGTTGGTTACGTTGAGTCTGGAAGCATCTGGACACAACAATTCATCGGAAAATAA
Reference Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed]
Miscellaneous promoter：P43