SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0001077
| Accession Number | SPSED0001077 |
| Secreted Protein | Cutinase |
| Uniprot Accession Number | P00590 |
| GenBank Accession Number | AAA33335.1 |
| Source Organism of the Secreted Protein | Fusarium solani pisi | Nucleotide Sequence of the Secreted Protein | Length = 693 ntATGAAATTCTTCGCTCTCACCACACTTCTCGCCGCCACGGCTTCGGCTCTGCCTACTTCTAACCCTGCCCAGGAGCTTGAGGCGCGCCAGCTTGGTAGAACAACTCGCGACGATCTGATCAACGGCAATAGCGCTTCCTGCGCCGATGTCATCTTCATTTATGCCCGAGGTTCAACAGAGACGGGCAACTTGGGAACTCTCGGTCCTAGCATTGCCTCCAACCTTGAGTCCGCCTTCGGCAAGGACGGTGTCTGGATTCAGGGCGTTGGCGGTGCCTACGCAGCCACTCTTGGAGACAATGCTCTCCCTCGCGGAACCTCTAGCGCCGCAATCAGGGAGATGCTCGGTCTCTTCCAGCAGGCCAACACCAAGTGCCCTGACGCGACTTTGATCGCCGGTGGCTACAGCCAGGGTGCTGCACTTGCAGCCGCCTCCATCGAGGACCTCGACTCGGCCATTCGTGACAAGATCGCCGGAACTGTTCTGTTCGGCTACACCAAGAACCTACAGAACCGTGGCCGAATCCCCAACTACCCTGCCGACAGGACCAAGGTCTTCTGCAATACAGGGGATCTCGTTTGTACTGGTAGCTTGATCGTTGCTGCACCTCACTTGGCTTATGGTCCTGATGCTCGTGGCCCTGCCCCTGAGTTCCTCATCGAGAAGGTTCGGGCTGTCCGTGGTTCTGCTTGA |
| Amino Acid Sequence of the Secreted Protein | Length = 230 aaMKFFALTTLLAATASALPTSNPAQELEARQLGRTTRDDLINGNSASCRDVIFIYARGSTETGNLGTLGPSIASNLESAFGKDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNTGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA |
| Expression Host | Bacillus subtilis TEB1030 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | ykoJ |
| Signal Peptide Sequence | Length = 26 aaMLKKKWMVGLLAGCLAAGGFSYNAFA |
| Type | Sec |
| Yield | 0.3 (U/ml) |
| Performance (Current Yield / Highest Yield) | 0.06 |
| Charge | 3 |
| Charge (N-Region) | 3 |
| Hydrophobicity | 0.79 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 1.56 |
| Signal Peptide Original Protein Sequence | MLKKKWMVGLLAGCLAAGGFSYNAFATENNENRQASSKTDALTEQEAEAIAKTVVDGTVEDIDRDLYNGKEVYEVEIEKEGEDYDVYVDIHTKQALNDPLKEKAEQVAITKEEAEEIALKQTGGTVTESKLDEDDGAYIYEMEIQTKQGTETEFEISAKDGRIIKQEIDD |
| Signal Peptide Original DNA Sequence | ATGCTCAAGAAAAAATGGATGGTCGGTCTTTTAGCAGGGTGCCTAGCGGCCGGAGGATTCAGCTACAACGCGTTTGCCACAGAAAACAATGAAAACAGACAGGCTTCTTCTAAGACGGATGCACTCACTGAACAAGAAGCAGAAGCAATTGCTAAAACAGTTGTTGACGGCACAGTAGAAGACATTGACAGAGACCTTTACAACGGCAAAGAAGTCTATGAAGTGGAGATTGAAAAAGAAGGTGAAGACTACGACGTTTATGTAGATATTCATACAAAACAAGCGCTTAATGACCCGTTAAAGGAGAAAGCAGAACAAGTCGCCATAACCAAAGAAGAAGCTGAAGAAATTGCTTTAAAGCAAACAGGTGGAACCGTAACAGAAAGTAAGCTTGACGAAGATGACGGTGCTTACATCTATGAAATGGAAATCCAAACGAAGCAAGGAACTGAGACAGAATTTGAAATTTCCGCAAAAGATGGACGCATTATCAAACAGGAGATAGATGACTAA |
| Reference | Brockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. [PubMed] |
| Miscellaneous | - |