SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0015002

Accession Number SPSED0015002
Secreted Protein L-Asparaginase
Uniprot Accession Number O34482
GenBank Accession Number BAA22230.1
Source Organism of the Secreted Protein Bacillus subtilis 168
Nucleotide Sequence of the Secreted Protein Length = 1128 nt
ATGAAAAAACAACGAATGCTCGTACTTTTTACCGCACTATTGTTTGTTTTTACCGGATGTTCACATTCTCCTGAAACAAAAGAATCCCCGAAAGAAAAAGCTCAGACACAAAAAGTCTCTTCGGCTTCTGCCTCTGAAAAAAAGGATCTGCCAAACATTAGAATTTTAGCGACAGGAGGCACGATAGCTGGTGCCGATCAATCGAAAACCTCAACAACTGAATATAAAGCAGGTGTTGTCGGCGTTGAATCACTGATCGAGGCAGTTCCAGAAATGAAGGACATTGCAAACGTCAGCGGCGAGCAGATTGTTAACGTCGGCAGCACAAATATTGATAATAAAATATTGCTGAAGCTGGCGAAACGCATCAACCACTTGCTCGCTTCAGATGATGTAGACGGAATCGTCGTGACTCATGGAACAGATACATTGGAGGAAACCGCTTATTTTTTGAATCTTACCGTGAAAAGTGATAAACCGGTTGTTATTGTCGGTTCGATGAGACCTTCCACAGCCATCAGCGCTGATGGGCCTTCTAACCTGTACAATGCAGTGAAAGTGGCAGGTGCCCCTGAGGCAAAAGGGAAAGGGACGCTTGTTGTTCTTAACGACCGGATTGCCTCAGCCCGATATGTCACCAAAACAAACACAACTACAACAGATACATTTAAATCAGAAGAAATGGGCTTCGTCGGAACAATTGCAGATGATATCTATTTTAATAATGAGATTACCCGTAAGCATACGAAGGACACGGATTTCTCGGTTTCTAATCTTGATGAGCTGCCGCAGGTTGACATTATCTATGGATACCAAAATGACGGAAGCTACCTGTTTGACGCTGCTGTAAAAGCCGGAGCAAAGGGGATTGTATTTGCCGGTTCTGGGAACGGGTCTTTATCTGATGCAGCCGAAAAAGGGGCGGACAGCGCAGTCAAAAAAGGCGTTACAGTGGTGCGCTCTACCCGCACGGGAAATGGTGTCGTCACACCAAACCAAGACTATGCGGAAAAGGACTTGCTGGCATCGAACTCTTTAAACCCCCAAAAAGCACGGATGTTGCTGATGCTTGCGCTTACCAAAACAAATGATCCTCAAAAAATCCAAGCTTATTTCAATGAGTATTGA
Amino Acid Sequence of the Secreted Protein Length = 375 aa
MKKQRMLVLFTALLFVFTGCSHSPETKESPKEKAQTQKVSSASASEKKDLPNIRILATGGTIAGADQSKTSTTEYKAGVVGVESLIEAVPEMKDIANVSGEQIVNVGSTNIDNKILLKLAKRINHLLASDDVDGIVVTHGTDTLEETAYFLNLTVKSDKPVVIVGSMRPSTAISADGPSNLYNAVKVAGAPEAKGKGTLVVLNDRIASARYVTKTNTTTTDTFKSEEMGFVGTIADDIYFNNEITRKHTKDTDFSVSNLDELPQVDIIYGYQNDGSYLFDAAVKAGAKGIVFAGSGNGSLSDAAEKGADSAVKKGVTVVRSTRTGNGVVTPNQDYAEKDLLASNSLNPQKARMLLMLALTKTNDPQKIQAYFNEY
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide wapA
Signal Peptide Sequence Length = 32 aa
MKKRKRRNFKRFIAAFLVLALMISLVPADVLA
Type Tat
Yield 28.9 (U/mL)
Performance (Current Yield / Highest Yield) 0.89
Charge 7
Charge (N-Region) 8
Hydrophobicity 0.59
Hydrophobicity Plot
Hydrophobicity (H-region) 2.69
Signal Peptide Original Protein Sequence MKKRKRRNFKRFIAAFLVLALMISLVPADVLAKSTEEENGNRIAADDPEETLQKEQTEEAVPFDPKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPDADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLAEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYNGYNTFTFQLKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKAYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMTVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNAKVTWNTKPASKNIGKADVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPTIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHLDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSAKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGSGAELPINPGPTYKNAGGDGAKRNYSFKIIAYNKDGEAIASPAATPALPDIARPKNVTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSFDVGDADHWTTQNKNIWPTSEEIKAGSYKLHTDGKGGELALDPSPVYNNANGNYKGKKNYSFTLVAYDANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDSSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYENDLLKKVTDTDGTVTSYDYDSEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHKDKDGKGKAWFDEVQLEKGEVSSSYNPVQNSSFTSATENWNVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQSANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGIYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGYATSTDYDETGKKTSETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKFEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYIFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWIHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGDILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTIKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGKVTNYFYDGDSLNVLYETDADNNVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDIIAISDSTGKTVAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGASRAYKFTKKAVKITGHTRHGLNQSIGRNGGRGVNLRAKLNAVRSPKKVIKQPNGATKYVGKKATVVLNKRGKVITAYGSSRAKGSKHVFHTHGKGNKSKRRR
Signal Peptide Original DNA Sequence ATGAAAAAAAGAAAGAGGCGAAACTTTAAAAGGTTCATTGCAGCATTTTTAGTGTTGGCTTTAATGATTTCATTAGTGCCAGCCGATGTACTAGCAAAATCTACAGAAGAAGAGAATGGAAACCGTATTGCTGCCGATGATCCAGAAGAAACTCTTCAGAAAGAACAAACTGAGGAAGCTGTACCTTTTGATCCAAAAGATATAAACAAAGAGGGTGAGATCACCTCTGAAAGAACAGAAAATACAAAACTCTATTATGAAGGTGACGGTGTCTATAAACAGGAAGTATACCTTGATCCTATTCATACAAAGGAAACACCGGATGCCGACTGGGAAGACATTTCTCCTGAGTTAAAAGAGTCGACAAGCAAACAAGTCGAGACAGAGAACGCTATTTTGAATTCAGATTTTCAAAAGCAAATGAAGAACGGGCTTTATGCGACTTTTGAGCATAATGACCATAAGGTTACATATTCACTAGCAGAAGCGAAAGGCCCGAATAAAACTTCACTTACTCCGAAAGATACCTCAGCAGATTATAAGACTGACAGTAATGAGATTGTGTATCCGGATGTGTTTCCGAATATTGATCTTCAGACTTTCACATTCAATGAAAACATAAAAGAAGATTTAGTCCTTCATCAGTATAATGGATACAACACATTTACATTTCAGTTAAAAACCGATTTGCAGGCTAAAGAACAAGAGGATGGATCCATTGATTTTTCTGATGAAAAAGGAAAAGTTGTTTTTTCTGTGCCTAAGCCTTTTATGACGGATTCAAAACTAGATGAGCTTTCCGGCGAGGTTGAACGGTCAGACAAAGTCAGCTATAAGCTGGAAAAGAATGAAGAAGGGTATTTGCTTCATCTTACGGCAGACGAGAACTGGCTGAAAGACCCAGAACGCGTATATCCAGTATCTATCGACCCGTCAACTTCTTTATCTGTTTCATCGGATACGTTTGTCATGAGTGCCTACCCGACGACGAACTATTCGGCCTCCTCACAAAAATGGGATGCTAATTTAAAGGCCTATGTACTGAAAACGGGCTATTATGACAAAACAACAGGCACAAACTATGCGTTCATGAAATTTAACAACCTAAAGCCAATTCAAAATATGACTGTCACTAAAGCTACATTAAAAACATACGTAGCCCATTCCTATTATGGAACAAAGGCAACCGGCCTTTGGCTGGATACGGTCAACAGCAATTATGATAATGCAAAAGTGACATGGAACACCAAACCGGCATCTAAAAATATCGGAAAAGCCGACGTCCATAAAGGACAGTGGGCGAGTTATGATGTGACCGCTGCTGTAAAGTCATGGAACTCCGGCGGGGCCAACTATGGATTTAAGCTTCATACAAACGGAAACGGCAAGGAATATTGGAAAAAACTAATTTCTTCAGCGAACTCCGCCAATAAACCATATATTGAAGTGACGTATACCATTCCAAAGGGAAACACACCTACAATCAAAGCCTATCACAACGGCGATTCCACTGGTTATTTCGATATCAGCTGGAAAAAGGTTGAAGGCGCAAAAGGATATAAGGTATGGATTTACAATGGTAAGGAATATCAAGCGATTTCTGCAGGCAACGTCACGTCGTGGAGCACAAAAGGTAAAAAAATCTGGCCTACGAGCGCAGAAATTGCCTCCAAGAGATATAAACTGCATCTGGACGGCAAGGATGGAGCTGAATTGGCATTAGATCCATCCCCGGTCTATAAGAATTCCGGCGGAAGCTATGCTACAAGCAAAAACTATTGGATTGGCGTCAGTGCGATATTTGACCAAGGTGAAGGAGCGATGTCCGCACCGGCAAAGCCCGTTATCCCTAATGTAGGAAAAGCACAAGCACCGTCAGCAAAGGGCTACAATAATGGGAATGCCACAGGATACTTCGACCTTTCTTGGAAAGCTGTATCCGGTGCAACCGGCTATAAGGTTCAGGTGTTCAATGGGAAAGGCTTTGAGACACTTGATCTCGGAAATCAGACGTCTTGGACCACAAAAGGGAAAAAGATCTGGCCGACAAGTGCAGAAATCAAGGCGGGAAAATATGCTCTTCACCTAAAGGACGGAAGCGGAGCAGAGCTGCCGATCAATCCCGGACCAACCTATAAAAACGCCGGGGGAGATGGAGCCAAAAGAAATTACTCGTTTAAAATTATCGCATACAACAAAGACGGCGAAGCCATTGCATCCCCGGCTGCTACCCCGGCACTTCCTGATATCGCTAGGCCAAAGAATGTAACTGGCTATTTGTATACAAATACAAAATCGAGTCAAACGGGTTATGTGAATTTGATATGGGAAAAGGTTCAAAATGCGAAGGGCTATAAAGTTAACATTTACAACGGTAAAGAATATCAGTCCTTTGATGTTGGTGATGCTGATCATTGGACAACCCAAAATAAAAACATCTGGCCGACTTCTGAGGAAATCAAAGCCGGAAGCTATAAGCTTCATACAGATGGAAAAGGCGGGGAATTAGCCCTCGATCCATCACCTGTCTATAACAATGCAAATGGGAATTACAAAGGGAAGAAGAATTATTCTTTCACGCTTGTCGCCTATGATGCGAATGGTGAAACGATTCCAACGGCGCCCTTTAACCCAACGTTCCATGAAGGCGCAGAATTCCTAGGTACAGAAGAGTACTGGTCTATCATTGATATCCCAAGCGGCCAGTTAAATGGTGCGACAGGCAATGTCATTGTGAATGAAGAAGATTTGTCAATTGATGGACGCGGTCCGGGACTTGGCTTATCGAGAACCTACAACAGTCTTGACAGCTCTGATCACTTATTTGGACAAGGCTGGTATGCTGATGCAGAAACATCTGTCATCTCAACGGATGGCGGGGCAATGTATATCGATGAAGACGCTACGACACATCGATTCACGAAAAAAGCCGATGGCACGTATCAGCCGCCGACCGGTGTATATCTTGAATTAACAGAAACAGCTGATCAGTTCATATTAAAAACAAAGGATCAAACCAACGCTTATTTCAATAAAAAAGGCGGCAAACTCCAAAAGGTAGTGGATGGCCATAACAATGCAACTGTCTATACTTACAATGACAAAAATCAACTGACCGCCATTACAGATGCATCAGGCCGCAAGCTTACATTTACCTATGATGAAAACGGCCATGTGACGTCCATCACAGGACCTAAGAATAAGAAAGTCACCTACTCATATGAGAATGATTTGCTGAAGAAGGTTACAGATACAGACGGGACTGTCACAAGCTATGACTACGATAGTGAAGGGCGCCTTGTCAAACAATATTCAGCTAACAGTACCGAAGCAAAGCCTGTCTTTACCGAATATCAGTATAGCGGTCACCGTCTCGAAAAAGCGATCAACGCTAAAAAAGAAACATATGTCTACAGCTATGATGCTGATAAAAAGACGCTTCTCATGACACAGCCAAATGGCCGGAAAGTACAGTATGGCTATAATGAAGCGGGAAATCCGATTCAGGTCATTGATGATGCGGAAGGGTTAAAAATTACAACGAATACAAAGTATGAAGGCAACAATGTTGTAGAAGATGTAGATCCAAATGACGTCGGCACCGGAAAAGCAACGGAAAGCTATCAATATGACAAAGATGGCAACGTTACGTCGGTTAAAGATGCATATGGAACTGAGACATACGAATACAACAAAAACAACGATGTCACAAAGATGAAGGATACCGAGGGCAATGTCACGGATATTGCTTATGACGGACTGGATGCTGTATCCGAAACCGATCAGAGCGGAAAATCATCGTCAGCAGCTGTTTATGATAAATATGGAAACCAGATTCAATCGAGCAAAGATTTATCAGCTTCAACTAATATTTTGAAAGATGGAAGCTTTGAAGCTCAAAAATCCGGCTGGAATCTGACTGCCTCTAAAGACAGCGGCAAAATTTCAGTCATCGCCGATAAAAGCGGAGTTCTTTCCGGATCAAAGGCATTGGAGGTCTTGAGCCAATCAACGTCTGCCGGAACAGATCATGGGTATTCGTCAGCAACACAAACAGTTGAGCTGGAGCCGAATACAACCTATACATTAAGCGGGAAAATCAAAACAGATCTTGCGAAATCAAGAGCCTATTTTAACATTGATTTGCGAGACAAAGATCAAAAACGAATTCAATGGATTCACAATGAATACAGTGCATTAGCCGGAAAAAATGACTGGACAAAACGGCAAATCACTTTTACGACTCCAGCGAATGCAGGCAAAGCTGTTGTGTATATGGAAGTCGATCATAAAGATAAAGATGGCAAAGGAAAAGCATGGTTTGATGAAGTCCAGCTGGAAAAAGGCGAAGTATCATCAAGCTATAACCCAGTTCAAAACAGCAGCTTCACATCAGCAACGGAAAACTGGAATGTAAGCGGAGCATCCGTTGATTCTGAAGAAGGCTTTAATGATGATGTATCATTAAAAGCTGCCCGGACCAGTGCGTCCCAAGCCGGATCAGTAACAAAACAAACCGTTGTCTTAGGGCAAAGCGCAAATGACAAACCGGTTTATCTCACACTGACAGGAATGTCGAAAGCAAGCAGCGTCAAATTTACAGATGAAAAAGACTATTCTCTGCAGGCGAATGTCACTTACGCTGACGGCAGCACAGGCATCTACAATGCGAAATTCCCTTCTGGAACACAGGAATGGAACCGAGCAGCTGTCGTCATCCCTAAAACAAAGCCGATCAATAAAGTCGATATCAGTATTCTGTTCCAAAAGAGTGCGACAGGCACAGTCTGGTTTGATGATATCCGTTTGATTGAAGGAAGCCTTCTGACTAAAAGCACGTATGACAGTAATGGCAACTATGTGACCAAGGAAGAGGACGAACTAGGTTACGCCACTTCAACTGATTACGATGAAACTGGTAAGAAAACGTCTGAAACAGATGCAAAAGGTGAAAAAACAACCTATACGTATGATCAGGCAGACCAATTGACAAACATGACGCTCTCTAACGGCACGTCTATCCTTCACAGCTATGATAAGGAAGGCAATGAGGTAAGCAAAACCATCCGGGCAGGAGCAGATCAAACCTATAAATTCGAATATGATGTCATGGGCAAGCTTGTCAAAACAACTGACCCTCTCGGCAATGTGTTAGCAAGCGAATATGATGCCAACAGCAACCTGACCAAAACCATTTCACCAAATGGCAACGAAGTGTCTCTTTCTTATGATGGAACAGACCGGGTGAAATCAAAGTCGTATAACGGTACAGAAAAGTACATTTTCACGTATGACAAAAATGGAAATGAAACGTCGGTCGTCAATAAAGAGCAGAACACCACAAAGAAACGGACATTTGACAACAAAAACCGTTTAACCGAACTGACAGACCGCGGCGGCAGCCAAACTTGGACATATCCTAGTGACTCCGATAAGCTGAAAACCTTCTCGTGGATTCATGGGGACCAAAAAGGAACGAATCAATTTACGTATAACAAGCTGGATCAAATGATTGAGATGAAAGACAGTACATCCTCATATTCATTTGATTATGATGAAAATGGAAACGTCCAAACCTTTATTACGGGCAACGGCGGCGGAACAAGTTTTTCATATGACGAAAGAAACCTTGTAAGCTCGCTGCATATCGGTGACAAAAACGGCGGAGACATTCTGACGGAAAGCTATGAATATGATGCCAACGGCAACCGCACAACGATAAACAGCTCAGCAAGCGGCAAGGTGCAATATGAGTATGGGAAGCTGAATCAGCTTGTCAAAGAAACCCATGAAGACGGTACTGTTATTGAATACACATATGATGGATTCGGAAACCGGAAAACCGTTACAACTATAAAAGACGGCTCAAGCAAAACGGTGAACGCTTCCTTCAACATCATGAACCAGCTGACAAAAGTCAATGATGAAAGCATCTCTTACGATAAAAACGGCAACCGCACATCCGACGGCAAATTCACATATACGTGGGATGCAGAAGATAACCTGACCGCCGTTACCAAAAAAGGCGAAGACAAGCCATTCGCAACATATAAGTATGACGAAAAAGGAAACAGAATCCAAAAAACCGTCAACGGAAAAGTCACGAACTACTTCTACGACGGAGACAGCTTGAACGTCTTGTATGAAACAGACGCGGACAACAACGTCACAAAATCATACACGTACGGAGACAGCGGCCAGCTGTTATCCTACACAGAAAATGGTAAGAAATATTTCTATCACTACAACGCTCACGGCGACATCATCGCGATCTCAGACAGCACTGGAAAAACCGTTGCCAAGTATCAATACGACGCATGGGGAAATCCAACAAAAACCGAAGCAAGCGACGAAGTAAAAGACAATCGCTACCGCTACGCAGGCTACCAATACGACGAAGAAACCGGCCTGTACTATCTCATGGCCCGCTACTACGAGCCAAGAAACGGCGTGTTCCTATCGCTCGACCCAGACCCGGGCAGCGACGGAGATTCATTGGATCAGAATGGGTATGCTTATGGAAATAATAACCCAGTGATGAATGTTGATCCGGACGGGCATTGGGTATGGCTTGTGGTAAATGCCGGATTTGCAGCCTATGATGGATATAAGGCATATAAATCAGGTAAAGGATGGAAAGGTGCTGCTTGGGCAGCTGCTTCTAATTTTGGTCCTGGGAAGATATTTAAGGGTGCTAGTCGCGCCTATAAGTTTACGAAGAAGGCAGTTAAAATCACAGGTCATACAAGACATGGACTTAATCAATCTATAGGAAGAAATGGAGGCCGTGGAGTCAATTTAAGGGCCAAACTGAATGCTGTGCGAAGCCCTAAAAAAGTAATAAAACAACCTAATGGGGCAACGAAATATGTTGGAAAAAAAGCTACAGTTGTTTTAAATAAAAGGGGTAAAGTAATCACAGCTTATGGAAGTTCAAGAGCTAAGGGTTCTAAACACGTATTCCATACACACGGAAAAGGAAATAAATCTAAGAGAAGGCGTTAA
Reference Feng Y, Liu S, Jiao Y, et al. Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B-subtilis WB600 through a combined strategy[J]. Applied Microbiology and Biotechnology, 2017, 101(4): 1509-1520 [PubMed]
Miscellaneous -