SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0015008

Accession Number SPSED0015008
Secreted Protein L-Asparaginase
Uniprot Accession Number O34482
GenBank Accession Number BAA22230.1
Source Organism of the Secreted Protein Bacillus subtilis 168
Nucleotide Sequence of the Secreted Protein Length = 1128 nt
ATGAAAAAACAACGAATGCTCGTACTTTTTACCGCACTATTGTTTGTTTTTACCGGATGTTCACATTCTCCTGAAACAAAAGAATCCCCGAAAGAAAAAGCTCAGACACAAAAAGTCTCTTCGGCTTCTGCCTCTGAAAAAAAGGATCTGCCAAACATTAGAATTTTAGCGACAGGAGGCACGATAGCTGGTGCCGATCAATCGAAAACCTCAACAACTGAATATAAAGCAGGTGTTGTCGGCGTTGAATCACTGATCGAGGCAGTTCCAGAAATGAAGGACATTGCAAACGTCAGCGGCGAGCAGATTGTTAACGTCGGCAGCACAAATATTGATAATAAAATATTGCTGAAGCTGGCGAAACGCATCAACCACTTGCTCGCTTCAGATGATGTAGACGGAATCGTCGTGACTCATGGAACAGATACATTGGAGGAAACCGCTTATTTTTTGAATCTTACCGTGAAAAGTGATAAACCGGTTGTTATTGTCGGTTCGATGAGACCTTCCACAGCCATCAGCGCTGATGGGCCTTCTAACCTGTACAATGCAGTGAAAGTGGCAGGTGCCCCTGAGGCAAAAGGGAAAGGGACGCTTGTTGTTCTTAACGACCGGATTGCCTCAGCCCGATATGTCACCAAAACAAACACAACTACAACAGATACATTTAAATCAGAAGAAATGGGCTTCGTCGGAACAATTGCAGATGATATCTATTTTAATAATGAGATTACCCGTAAGCATACGAAGGACACGGATTTCTCGGTTTCTAATCTTGATGAGCTGCCGCAGGTTGACATTATCTATGGATACCAAAATGACGGAAGCTACCTGTTTGACGCTGCTGTAAAAGCCGGAGCAAAGGGGATTGTATTTGCCGGTTCTGGGAACGGGTCTTTATCTGATGCAGCCGAAAAAGGGGCGGACAGCGCAGTCAAAAAAGGCGTTACAGTGGTGCGCTCTACCCGCACGGGAAATGGTGTCGTCACACCAAACCAAGACTATGCGGAAAAGGACTTGCTGGCATCGAACTCTTTAAACCCCCAAAAAGCACGGATGTTGCTGATGCTTGCGCTTACCAAAACAAATGATCCTCAAAAAATCCAAGCTTATTTCAATGAGTATTGA
Amino Acid Sequence of the Secreted Protein Length = 375 aa
MKKQRMLVLFTALLFVFTGCSHSPETKESPKEKAQTQKVSSASASEKKDLPNIRILATGGTIAGADQSKTSTTEYKAGVVGVESLIEAVPEMKDIANVSGEQIVNVGSTNIDNKILLKLAKRINHLLASDDVDGIVVTHGTDTLEETAYFLNLTVKSDKPVVIVGSMRPSTAISADGPSNLYNAVKVAGAPEAKGKGTLVVLNDRIASARYVTKTNTTTTDTFKSEEMGFVGTIADDIYFNNEITRKHTKDTDFSVSNLDELPQVDIIYGYQNDGSYLFDAAVKAGAKGIVFAGSGNGSLSDAAEKGADSAVKKGVTVVRSTRTGNGVVTPNQDYAEKDLLASNSLNPQKARMLLMLALTKTNDPQKIQAYFNEY
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide nprE
Signal Peptide Sequence Length = 27 aa
MGLGKKLSVAVAASFMSLSISLPGVQA
Type Sec
Yield 8 (U/mL)
Performance (Current Yield / Highest Yield) 0.25
Charge 2
Charge (N-Region) 2
Hydrophobicity 1.04
Hydrophobicity Plot
Hydrophobicity (H-region) 1.58
Signal Peptide Original Protein Sequence MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKLVESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAVSNGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNISYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALTTYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
Signal Peptide Original DNA Sequence GTGGGTTTAGGTAAGAAATTGTCTGTTGCTGTCGCTGCTTCGTTTATGAGTTTATCAATCAGCCTGCCAGGTGTTCAGGCTGCTGAAGGTCATCAGCTTAAAGAGAATCAAACAAATTTCCTCTCCAAAAACGCGATTGCGCAATCAGAACTCTCTGCACCAAATGACAAGGCTGTCAAGCAGTTTTTGAAAAAGAACAGCAACATTTTTAAAGGTGACCCTTCCAAAAGGCTGAAGCTTGTTGAAAGCACGACTGATGCCCTTGGATACAAGCACTTTCGATATGCGCCTGTCGTTAACGGAGTGCCAATTAAAGATTCGCAAGTGATCGTTCACGTCGATAAATCCGATAATGTCTATGCGGTCAATGGTGAATTACACAATCAATCTGCTGCAAAAACAGATAACAGCCAAAAAGTCTCTTCTGAAAAAGCGCTGGCACTCGCTTTCAAAGCTATCGGCAAATCACCAGACGCTGTTTCTAACGGAGCGGCCAAAAACAGCAATAAAGCCGAATTAAAAGCGATAGAAACAAAAGACGGCAGCTATCGTCTTGCTTACGACGTGACGATTCGCTATGTCGAGCCTGAACCTGCAAACTGGGAAGTCTTAGTTGACGCCGAAACAGGCAGCATTTTAAAACAGCAAAATAAAGTAGAACATGCCGCCGCCACTGGAAGCGGAACAACGCTAAAGGGCGCAACTGTTCCTTTGAACATCTCTTATGAAGGCGGAAAATATGTTCTAAGAGATCTTTCAAAACCAACAGGCACCCAAATCATCACATATGATTTGCAAAACAGACAAAGCCGCCTTCCGGGCACGCTTGTCTCAAGCACAACGAAAACATTTACATCTTCATCACAGCGGGCAGCCGTTGACGCACACTATAACCTCGGTAAAGTGTACGATTATTTTTATTCAAACTTTAAACGAAACAGCTATGATAACAAAGGCAGTAAAATCGTTTCTTCCGTTCACTACGGCACTCAATACAATAACGCTGCATGGACAGGAGACCAGATGATTTACGGTGATGGCGACGGTTCATTCTTCTCTCCGCTTTCCGGCTCATTAGATGTGACAGCGCATGAAATGACACATGGCGTCACCCAAGAAACAGCCAACTTGATTTATGAAAATCAGCCAGGTGCATTAAACGAGTCTTTCTCTGACGTATTCGGGTATTTTAACGATACAGAAGACTGGGACATCGGTGAAGACATTACGGTCAGCCAGCCTGCTCTTCGCAGCCTGTCCAACCCTACAAAATACAACCAGCCTGACAATTACGCCAATTACCGAAACCTTCCAAACACAGATGAAGGCGATTATGGCGGTGTACACACAAACAGCGGAATTCCAAACAAAGCCGCTTACAACACCATCACAAAACTTGGTGTATCTAAATCACAGCAAATCTATTACCGTGCGTTAACAACGTACCTCACGCCTTCTTCCACGTTCAAAGATGCCAAGGCAGCTCTCATTCAGTCTGCCCGTGACCTCTACGGCTCAACTGATGCCGCTAAAGTTGAAGCAGCCTGGAATGCTGTTGGATTGTAA
Reference Xuan Chen.Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600[D]. Jiangnan University, 2015. (in Chinese)
Miscellaneous -