SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0015013

Accession Number SPSED0015013
Secreted Protein L-Asparaginase
Uniprot Accession Number O34482
GenBank Accession Number BAA22230.1
Source Organism of the Secreted Protein Bacillus subtilis 168
Nucleotide Sequence of the Secreted Protein Length = 1128 nt
ATGAAAAAACAACGAATGCTCGTACTTTTTACCGCACTATTGTTTGTTTTTACCGGATGTTCACATTCTCCTGAAACAAAAGAATCCCCGAAAGAAAAAGCTCAGACACAAAAAGTCTCTTCGGCTTCTGCCTCTGAAAAAAAGGATCTGCCAAACATTAGAATTTTAGCGACAGGAGGCACGATAGCTGGTGCCGATCAATCGAAAACCTCAACAACTGAATATAAAGCAGGTGTTGTCGGCGTTGAATCACTGATCGAGGCAGTTCCAGAAATGAAGGACATTGCAAACGTCAGCGGCGAGCAGATTGTTAACGTCGGCAGCACAAATATTGATAATAAAATATTGCTGAAGCTGGCGAAACGCATCAACCACTTGCTCGCTTCAGATGATGTAGACGGAATCGTCGTGACTCATGGAACAGATACATTGGAGGAAACCGCTTATTTTTTGAATCTTACCGTGAAAAGTGATAAACCGGTTGTTATTGTCGGTTCGATGAGACCTTCCACAGCCATCAGCGCTGATGGGCCTTCTAACCTGTACAATGCAGTGAAAGTGGCAGGTGCCCCTGAGGCAAAAGGGAAAGGGACGCTTGTTGTTCTTAACGACCGGATTGCCTCAGCCCGATATGTCACCAAAACAAACACAACTACAACAGATACATTTAAATCAGAAGAAATGGGCTTCGTCGGAACAATTGCAGATGATATCTATTTTAATAATGAGATTACCCGTAAGCATACGAAGGACACGGATTTCTCGGTTTCTAATCTTGATGAGCTGCCGCAGGTTGACATTATCTATGGATACCAAAATGACGGAAGCTACCTGTTTGACGCTGCTGTAAAAGCCGGAGCAAAGGGGATTGTATTTGCCGGTTCTGGGAACGGGTCTTTATCTGATGCAGCCGAAAAAGGGGCGGACAGCGCAGTCAAAAAAGGCGTTACAGTGGTGCGCTCTACCCGCACGGGAAATGGTGTCGTCACACCAAACCAAGACTATGCGGAAAAGGACTTGCTGGCATCGAACTCTTTAAACCCCCAAAAAGCACGGATGTTGCTGATGCTTGCGCTTACCAAAACAAATGATCCTCAAAAAATCCAAGCTTATTTCAATGAGTATTGA
Amino Acid Sequence of the Secreted Protein Length = 375 aa
MKKQRMLVLFTALLFVFTGCSHSPETKESPKEKAQTQKVSSASASEKKDLPNIRILATGGTIAGADQSKTSTTEYKAGVVGVESLIEAVPEMKDIANVSGEQIVNVGSTNIDNKILLKLAKRINHLLASDDVDGIVVTHGTDTLEETAYFLNLTVKSDKPVVIVGSMRPSTAISADGPSNLYNAVKVAGAPEAKGKGTLVVLNDRIASARYVTKTNTTTTDTFKSEEMGFVGTIADDIYFNNEITRKHTKDTDFSVSNLDELPQVDIIYGYQNDGSYLFDAAVKAGAKGIVFAGSGNGSLSDAAEKGADSAVKKGVTVVRSTRTGNGVVTPNQDYAEKDLLASNSLNPQKARMLLMLALTKTNDPQKIQAYFNEY
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide oppA
Signal Peptide Sequence Length = 20 aa
MKKRWSIVTLMLIFTLVLSA
Type Sec
Yield 0 (U/mL)
Performance (Current Yield / Highest Yield) 0
Charge 3
Charge (N-Region) 3
Hydrophobicity 1.24
Hydrophobicity Plot
Hydrophobicity (H-region) 2.34
Signal Peptide Original Protein Sequence MKKRWSIVTLMLIFTLVLSACGFGGSGSNGEGKKDSKGKTTLNINIKTEPFSLHPGLANDSVSGGVIRQTFEGLTRINADGEPEEGMASKIETSKDGKTYTFTIRDGVKWSNGDPVTAQDFEYAWKWALDPNNESQYAYQLYYIKGAEAANTGKGSLDDVAVKAVNDKTLKVELNNPTPYFTELTAFYTYMPINKKIAEKNKKWNTNAGDDYVSNGPFKMTAWKHSGSITLEKNDQYWDKDKVKLKKIDMVMINNNNTELKKFQAGELDWAGMPLGQLPTESLPTLKKDGSLHVEPIAGVYWYKFNTEAKPLDNVNIRKALTYSLDRQSIVKNVTQGEQIPAMAAVPPTMKGFEDNKEGYFKDNDVKTAKEYLEKGLKEMGLSKASDLPKIKLSYNTDDAHAKIAQAVQEMWKKNLGVDVELDNSEWNVYIDKLHSQDYQIGRMGWLGDFNDPINFLELFRDKNGGNNDTGWENPEFKKLLNQSQTETDKTKRAELLKKAEGIFIDEMPVAPIYFYTDTWVQDENLKGVIMPGTGEVYFRNAYFK
Signal Peptide Original DNA Sequence ATGAAAAAACGTTGGTCGATTGTCACGTTGATGCTCATTTTCACTCTCGTGCTGAGCGCGTGCGGCTTTGGCGGCAGCGGATCAAACGGTGAAGGGAAAAAGGACAGTAAAGGAAAGACGACACTTAACATTAATATTAAAACTGAGCCGTTCTCCTTACATCCGGGATTGGCAAATGATTCAGTATCAGGCGGTGTTATCCGTCAGACTTTTGAAGGATTGACACGTATCAATGCAGATGGTGAGCCTGAAGAAGGCATGGCTTCTAAAATTGAAACGAGCAAGGACGGAAAGACATATACATTTACCATTCGTGATGGTGTGAAATGGTCTAATGGAGACCCTGTAACTGCACAAGATTTTGAATATGCTTGGAAATGGGCGCTTGACCCTAATAATGAATCACAATACGCTTACCAGCTCTACTACATAAAAGGTGCTGAAGCGGCGAATACCGGAAAAGGCAGCCTAGACGATGTGGCAGTAAAAGCTGTGAATGACAAAACGCTGAAGGTTGAATTAAATAACCCGACTCCATATTTCACTGAATTAACTGCGTTCTATACGTATATGCCGATCAATAAGAAAATTGCAGAGAAAAATAAAAAGTGGAATACAAATGCCGGAGATGATTATGTATCAAACGGGCCGTTCAAAATGACGGCATGGAAACACAGCGGCTCTATTACTCTCGAAAAAAATGACCAGTATTGGGATAAAGACAAAGTCAAACTGAAGAAAATCGATATGGTTATGATCAACAATAACAATACGGAACTAAAAAAATTCCAAGCTGGCGAACTTGATTGGGCCGGTATGCCGCTCGGACAGCTTCCGACAGAATCCCTGCCGACCCTGAAAAAAGACGGTTCTTTACATGTTGAGCCGATTGCAGGAGTGTATTGGTACAAATTCAACACTGAAGCTAAGCCATTAGACAACGTCAATATCCGTAAAGCTTTAACATATTCGCTTGACCGTCAGTCGATTGTTAAAAACGTTACGCAAGGAGAGCAAATCCCGGCAATGGCTGCAGTGCCGCCTACAATGAAGGGATTTGAGGATAACAAAGAAGGATACTTCAAAGACAATGATGTCAAAACAGCAAAAGAATACCTTGAAAAAGGCCTAAAAGAAATGGGCTTAAGCAAGGCATCTGATTTGCCAAAAATCAAATTGTCTTACAACACTGATGACGCACACGCGAAAATCGCTCAAGCAGTACAAGAAATGTGGAAGAAAAATTTAGGCGTTGATGTTGAGCTTGATAACTCAGAGTGGAATGTCTATATTGATAAGCTCCACAGCCAAGATTATCAAATCGGCCGTATGGGCTGGCTCGGCGACTTCAATGATCCTATCAACTTCCTTGAATTGTTCCGCGACAAAAACGGAGGAAATAACGATACAGGCTGGGAAAATCCAGAATTCAAAAAGCTTCTGAATCAGTCACAAACTGAAACAGATAAAACAAAACGTGCAGAGCTGCTGAAAAAAGCAGAAGGTATTTTCATTGATGAAATGCCGGTTGCCCCAATCTATTTCTATACTGATACTTGGGTACAGGATGAAAACCTAAAAGGTGTTATCATGCCAGGTACTGGTGAGGTTTATTTCAGAAACGCATATTTTAAATAA
Reference Xuan Chen.Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600[D]. Jiangnan University, 2015. (in Chinese)
Miscellaneous -