SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0016014

Accession Number SPSED0016014
Secreted Protein Alkaline protease (alk)
Uniprot Accession Number P41362
GenBank Accession Number AAC60420.1
Source Organism of the Secreted Protein Bacillus alcalophilus
Nucleotide Sequence of the Secreted Protein Length = 1143 nt
ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA
Amino Acid Sequence of the Secreted Protein Length = 380 aa
MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus licheniformis
Source Gene of the Signal Peptide matE
Signal Peptide Sequence Length = 31 aa
MRMKRLRMRKHLLIAVCTLALLLSSPIVSDA
Type Tat
Yield 5160.24 (U/mL)
Performance (Current Yield / Highest Yield) 0.68
Charge 5
Charge (N-Region) 6
Hydrophobicity 0.66
Hydrophobicity Plot
Hydrophobicity (H-region) 2.78
Signal Peptide Original Protein Sequence MRMKRLRMRKHLLIAVCTLALLLSSPIVSDASPATKPTTADSPQSSGFFVDHYKNNISANTTAESNPVIGLLSEFNKLWTPGKTWNTGTKLNSRVLDANIQKVVDIAERRTMLEENAAYFDDRRSQSYSIIDGLGKLAGVYRMNAGATTTITSIPADASIRKYNDEGTNSGSTSSELGNVVSLVNTLRGNYSSSNPAKSYFNYPRPFRWKDNSIIVPTLIPVINPDPNKDGGFPSGHTNAAYLSAFAMAYAIPERYQELLTRASELGHNRIVAGMHSPLDVMGGRVMATALSAAILSDPANERLKKTAFDEARRKLLTQTGTAEDRYSDYEKNKKQYTERLTYGFRQMNKTAKPMAVPKGAEVLLETRFPYLDKKQRRSVLATTGLPAGYPVLDDREGWGRLNLFSAADGYGAFTKNVTVTMDSAKGGFHTADRWRNDISGTGKLTKKGTGALKLEGDNTYSGGTRIDQGTLEGGSETAFGRGDVALNGGILKEDAPGKLIIEGDYKQSAKGILELQLSGKKDQLKIKGKARLKGTLRLNFTDNYVPADGSAIITFRKRHGSFSSVETSGLPSKYKVKIIYKSNSIQLKVEQKGRS
Signal Peptide Original DNA Sequence GTGAGAATGAAACGATTAAGGATGAGGAAGCATTTACTGATAGCTGTCTGTACTTTGGCACTTCTTCTAAGTTCCCCGATTGTAAGCGATGCGAGCCCGGCAACTAAACCAACAACTGCAGATTCGCCGCAATCTTCCGGATTTTTCGTAGACCATTACAAAAATAATATCTCTGCCAATACGACGGCGGAATCCAATCCTGTCATCGGCCTGCTTTCCGAATTTAATAAACTTTGGACTCCCGGAAAGACATGGAATACCGGTACTAAACTGAACAGCAGGGTGCTGGATGCCAACATTCAAAAAGTCGTGGATATTGCTGAACGCCGCACGATGCTTGAGGAAAATGCTGCCTATTTTGATGATCGGCGGAGCCAGAGCTACAGTATAATTGACGGCCTCGGCAAGCTTGCCGGCGTCTATCGAATGAACGCGGGAGCGACGACAACGATCACCAGCATTCCGGCAGATGCCTCGATTAGAAAATACAATGATGAAGGAACCAATTCGGGCAGCACCAGCTCTGAACTTGGAAATGTCGTAAGTTTGGTCAATACTTTACGCGGCAACTATTCTTCATCGAATCCGGCTAAAAGCTATTTCAACTATCCCCGCCCGTTTCGCTGGAAAGACAATTCGATCATTGTTCCAACGCTTATCCCCGTCATCAATCCTGATCCGAACAAAGACGGAGGTTTTCCAAGCGGACACACGAACGCCGCATATCTCAGCGCTTTTGCTATGGCCTATGCGATACCGGAGCGTTATCAGGAGCTGCTGACTCGCGCTTCAGAACTCGGTCATAACCGGATTGTTGCCGGTATGCATTCCCCGCTGGACGTCATGGGGGGACGAGTAATGGCAACAGCTTTGTCTGCAGCAATCCTGTCTGACCCCGCAAATGAAAGATTGAAGAAAACGGCTTTTGATGAAGCCCGCCGTAAATTATTAACGCAAACCGGTACAGCTGAAGACAGATACAGCGATTATGAGAAGAATAAAAAACAATATACGGAACGATTGACATATGGATTTCGACAAATGAACAAAACCGCCAAACCAATGGCAGTTCCAAAGGGAGCCGAAGTCCTGCTGGAAACACGTTTTCCTTACCTTGACAAAAAGCAGCGCCGTTCGGTTTTAGCCACTACCGGTCTTCCGGCCGGCTACCCTGTTCTTGATGATCGAGAAGGATGGGGAAGGCTTAATCTCTTTTCCGCGGCAGATGGGTATGGGGCTTTTACCAAAAATGTTACCGTGACCATGGATTCCGCAAAAGGCGGCTTCCATACAGCCGATCGCTGGCGCAACGACATCTCCGGCACCGGAAAGCTGACCAAAAAAGGGACAGGCGCTTTGAAGCTGGAAGGGGATAATACATATTCCGGCGGTACACGGATTGATCAAGGAACACTTGAGGGCGGTTCGGAGACAGCTTTCGGGAGAGGTGATGTTGCACTAAACGGAGGCATCCTTAAGGAAGATGCGCCGGGAAAACTGATCATCGAAGGAGACTACAAACAATCTGCTAAAGGAATACTTGAACTTCAGCTCAGCGGCAAAAAAGATCAGTTGAAAATTAAGGGAAAAGCAAGATTGAAAGGGACATTGCGTCTCAATTTTACGGACAATTACGTACCGGCTGACGGATCGGCGATCATAACCTTCCGCAAGCGTCATGGATCATTTTCTTCCGTCGAGACCAGTGGATTGCCAAGCAAGTATAAAGTGAAGATCATCTATAAATCCAACAGTATTCAGTTGAAAGTTGAGCAAAAGGGGAGAAGCTGA
Reference Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed]
Miscellaneous -