SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0016015

Accession Number SPSED0016015
Secreted Protein Alkaline protease (alk)
Uniprot Accession Number P41362
GenBank Accession Number AAC60420.1
Source Organism of the Secreted Protein Bacillus alcalophilus
Nucleotide Sequence of the Secreted Protein Length = 1143 nt
ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA
Amino Acid Sequence of the Secreted Protein Length = 380 aa
MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis 168
Source Gene of the Signal Peptide yngK
Signal Peptide Sequence Length = 30 aa
MKVCQKSIVRFLVSLIIGTFVISVPFMANA
Type Sec
Yield 4755.07 (U/mL)
Performance (Current Yield / Highest Yield) 0.63
Charge 3
Charge (N-Region) 3
Hydrophobicity 1.35
Hydrophobicity Plot
Hydrophobicity (H-region) 2.34
Signal Peptide Original Protein Sequence MKVCQKSIVRFLVSLIIGTFVISVPFMANAQSDRELRAVWIASVLNIDWPSKKGLSVKEQKQEYIKLLDDVQKMGMNAVIVQIKPTADAFYPSAYGPWSEYLTGVQGKDPGYDPLAFMIEETHKRNLEFHAWFNPYRITMNHTDLNKLSEDHPARKHPDWVAAYGNQLYYHPGIPEARDFIVKGIEEVVKHYDIDAVHMDDYFYPYKIAGQEFPDQAQYEQYGKDAFSNIDDWRRDNVNQLVKQINQTIKAAKPYVKFGISPFGVWRNAADDPTGSNTKAGVRNYDDLYADTRHWIQEGDIDYIAPQIYWSIGFNAAAYDVLADWWSNEVKNRPVHLYIGQAAYKINNNFDPPWSDPEEYVRQITLNRQLELVKGSMHFSLKDLNKNPLGIKDSLSTDLYSKPALVPQMPWLDNTAPKKPKLTKVTEDKNGNLLQIKDHPSNQKTKETAYYAIYRAEGKKQRTLLATQRKTHEQQTFLDNTADPNKKYTYYVTSADRLHNESKASKRTTK
Signal Peptide Original DNA Sequence ATGAAGGTTTGCCAAAAGTCGATTGTCCGTTTCCTTGTCAGCTTGATCATCGGAACGTTTGTCATTTCCGTTCCGTTTATGGCCAATGCGCAGTCTGACAGGGAGCTGAGAGCCGTATGGATTGCTTCCGTATTAAATATTGATTGGCCGTCCAAAAAAGGGTTATCTGTGAAAGAGCAAAAACAGGAGTATATCAAACTGCTGGACGATGTGCAAAAAATGGGAATGAATGCCGTTATCGTTCAGATTAAACCGACGGCTGATGCCTTTTATCCGTCAGCTTACGGGCCTTGGTCGGAATACTTAACCGGTGTCCAAGGGAAGGACCCCGGCTATGATCCTCTTGCATTTATGATTGAAGAAACCCATAAACGAAATTTGGAATTTCACGCGTGGTTTAATCCTTACCGTATTACAATGAATCATACAGATCTTAATAAATTGTCTGAGGATCACCCGGCAAGAAAACATCCAGATTGGGTTGCCGCCTATGGAAACCAGCTGTATTATCATCCAGGCATTCCAGAAGCGAGAGATTTTATCGTCAAAGGCATTGAAGAAGTCGTAAAACATTATGATATCGATGCTGTTCATATGGATGATTATTTTTACCCTTATAAAATAGCGGGACAGGAATTTCCTGATCAAGCACAGTACGAACAATACGGAAAAGACGCTTTTTCTAATATCGATGACTGGCGGCGGGACAATGTGAACCAGCTTGTCAAACAAATCAACCAAACAATTAAAGCTGCAAAACCGTATGTCAAATTCGGCATCAGTCCCTTTGGAGTATGGAGAAACGCCGCAGATGACCCGACCGGATCGAATACAAAAGCAGGCGTCAGAAATTATGACGATCTATATGCAGACACAAGGCATTGGATCCAAGAAGGCGACATCGATTATATTGCTCCGCAAATTTATTGGAGTATCGGCTTTAACGCAGCGGCTTACGACGTGCTGGCTGATTGGTGGAGCAATGAAGTCAAAAATAGGCCTGTTCACTTATATATTGGCCAGGCAGCATATAAAATCAATAACAATTTTGATCCGCCTTGGTCCGATCCTGAGGAATATGTGCGGCAAATCACTTTGAACCGCCAGCTTGAACTGGTGAAAGGAAGCATGCATTTCAGCCTCAAGGACCTCAACAAAAATCCGCTTGGCATCAAAGACAGTCTCAGCACCGATCTTTACAGCAAGCCGGCACTGGTTCCGCAAATGCCTTGGCTTGACAATACCGCCCCAAAAAAACCGAAGCTTACGAAAGTCACCGAAGACAAAAACGGCAATCTTCTGCAGATTAAAGATCACCCATCAAATCAAAAAACGAAAGAAACAGCCTATTATGCGATATACAGAGCCGAAGGGAAAAAGCAGAGAACATTGCTGGCAACGCAAAGAAAAACACATGAGCAGCAAACCTTCTTAGATAACACGGCAGATCCCAACAAGAAATATACGTATTATGTCACTTCAGCAGACCGGCTTCATAACGAAAGCAAAGCTTCCAAACGAACAACAAAATAA
Reference Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed]
Miscellaneous -