SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0016021

Accession Number SPSED0016021
Secreted Protein Alkaline protease (alk)
Uniprot Accession Number P41362
GenBank Accession Number AAC60420.1
Source Organism of the Secreted Protein Bacillus alcalophilus
Nucleotide Sequence of the Secreted Protein Length = 1143 nt
ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA
Amino Acid Sequence of the Secreted Protein Length = 380 aa
MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis 168
Source Gene of the Signal Peptide vpr
Signal Peptide Sequence Length = 29 aa
MKKGIIRFLLVSFVLFFALSTGITGVQAA
Type Sec
Yield 2369.89 (U/mL)
Performance (Current Yield / Highest Yield) 0.31
Charge 3
Charge (N-Region) 3
Hydrophobicity 1.37
Hydrophobicity Plot
Hydrophobicity (H-region) 2.69
Signal Peptide Original Protein Sequence MKKGIIRFLLVSFVLFFALSTGITGVQAAPASSKTSADLEKAEVFGDIDMTTSKKTTVIVELKEKSLAEAKEAGESQSKSKLKTARTKAKNKAIKAVKNGKVNREYEQVFSGFSMKLPANEIPKLLAVKDVKAVYPNVTYKTDNMKDKDVTISEDAVSPQMDDSAPYIGANDAWDLGYTGKGIKVAIIDTGVEYNHPDLKKNFGQYKGYDFVDNDYDPKETPTGDPRGEATDHGTHVAGTVAANGTIKGVAPDATLLAYRVLGPGGSGTTENVIAGVERAVQDGADVMNLSLGNSLNNPDWATSTALDWAMSEGVVAVTSNGNSGPNGWTVGSPGTSREAISVGATQLPLNEYAVTFGSYSSAKVMGYNKEDDVKALNNKEVELVEAGIGEAKDFEGKDLTGKVAVVKRGSIAFVDKADNAKKAGAIGMVVYNNLSGEIEANVPGMSVPTIKLSLEDGEKLVSALKAGETKTTFKLTVSKALGEQVADFSSRGPVMDTWMIKPDISAPGVNIVSTIPTHDPDHPYGYGSKQGTSMASPHIAGAVAVIKQAKPKWSVEQIKAAIMNTAVTLKDSDGEVYPHNAQGAGSARIMNAIKADSLVSPGSYSYGTFLKENGNETKNETFTIENQSSIRKSYTLEYSFNGSGISTSGTSRVVIPAHQTGKATAKVKVNTKKTKAGTYEGTVIVREGGKTVAKVPTLLIVKEPDYPRVTSVSVSEGSVQGTYQIETYLPAGAEELAFLVYDSNLDFAGQAGIYKNQDKGYQYFDWDGTINGGTKLPAGEYYLLAYAANKGKSSQVLTEEPFTVE
Signal Peptide Original DNA Sequence TTGAAAAAGGGGATCATTCGCTTTCTGCTTGTAAGTTTCGTCTTATTTTTTGCGTTATCCACAGGCATTACGGGCGTTCAGGCAGCTCCGGCTTCTTCAAAAACGTCGGCTGATCTGGAAAAAGCCGAGGTATTCGGTGATATCGATATGACGACAAGCAAAAAAACAACCGTTATAGTGGAATTAAAAGAAAAATCCTTGGCAGAAGCGAAGGAAGCGGGAGAAAGCCAATCGAAAAGCAAGCTGAAAACCGCTCGCACCAAAGCAAAAAACAAAGCAATCAAAGCAGTGAAAAACGGAAAAGTAAACCGGGAATATGAGCAGGTATTCTCAGGCTTCTCTATGAAGCTTCCAGCTAATGAGATTCCAAAACTTCTAGCGGTAAAAGACGTTAAGGCAGTGTACCCGAACGTCACATATAAAACAGACAATATGAAGGATAAAGACGTCACAATCTCCGAAGACGCCGTATCTCCGCAAATGGATGACAGTGCGCCTTATATCGGAGCAAACGATGCATGGGATTTAGGCTACACAGGAAAAGGCATCAAGGTGGCGATTATTGACACTGGGGTTGAATACAATCACCCAGATCTGAAGAAAAACTTTGGACAATATAAAGGATACGATTTTGTGGACAATGATTACGATCCAAAAGAAACACCAACCGGCGATCCGAGGGGCGAGGCAACTGACCATGGCACACACGTAGCCGGAACTGTGGCTGCAAACGGAACGATTAAAGGCGTAGCGCCTGATGCCACACTTCTTGCTTATCGTGTGTTAGGGCCTGGCGGAAGCGGCACAACGGAAAACGTCATCGCGGGCGTGGAACGTGCAGTGCAGGACGGGGCAGATGTGATGAACCTGTCTCTCGGAAACTCTTTAAACAACCCGGACTGGGCGACAAGCACAGCGCTTGACTGGGCCATGTCAGAAGGCGTTGTCGCTGTTACCTCAAACGGCAACAGCGGACCGAACGGCTGGACAGTCGGATCGCCGGGCACATCAAGAGAAGCGATTTCTGTCGGTGCGACTCAGCTGCCGCTCAATGAATACGCCGTCACTTTCGGCTCCTACTCTTCAGCAAAAGTGATGGGCTACAACAAAGAGGACGACGTCAAAGCGCTCAATAACAAAGAAGTTGAGCTTGTCGAAGCGGGAATCGGCGAAGCAAAGGATTTTGAAGGGAAAGACCTGACAGGCAAAGTCGCCGTTGTCAAACGAGGCAGCATTGCATTTGTGGATAAAGCGGATAACGCTAAAAAAGCCGGTGCAATCGGCATGGTTGTGTATAACAACCTCTCTGGAGAAATTGAAGCCAATGTGCCAGGCATGTCTGTCCCAACGATTAAGCTTTCATTAGAAGACGGCGAAAAACTCGTCAGCGCCCTGAAAGCTGGTGAGACAAAAACAACATTCAAGTTGACGGTCTCAAAAGCGCTCGGTGAACAAGTCGCTGATTTCTCATCACGCGGCCCTGTTATGGATACGTGGATGATTAAGCCTGATATTTCCGCGCCAGGGGTCAATATCGTGAGCACGATCCCAACACACGATCCTGACCATCCATACGGCTACGGATCAAAACAAGGAACAAGCATGGCATCGCCTCATATTGCCGGAGCGGTTGCCGTTATTAAACAAGCCAAACCAAAGTGGAGCGTTGAACAGATTAAAGCCGCCATCATGAATACCGCTGTCACTTTAAAGGATAGCGATGGGGAAGTATATCCGCATAACGCTCAAGGCGCAGGCAGCGCAAGAATTATGAACGCAATCAAAGCCGATTCGCTCGTCTCACCTGGAAGCTATTCATACGGCACGTTCTTGAAGGAAAACGGAAACGAAACAAAAAATGAAACGTTTACGATTGAAAATCAATCTTCCATTAGAAAGTCATACACACTTGAATACTCATTTAATGGCAGCGGCATTTCCACATCCGGCACAAGCCGTGTTGTGATTCCGGCACATCAAACCGGGAAAGCCACTGCAAAAGTAAAGGTCAATACGAAGAAAACAAAAGCTGGCACCTATGAAGGAACGGTTATCGTCAGAGAAGGCGGAAAAACGGTCGCTAAGGTACCTACATTGCTGATTGTGAAAGAGCCCGATTATCCGAGAGTCACATCTGTCTCTGTCAGCGAAGGGTCTGTACAAGGTACCTATCAAATTGAAACCTACCTTCCTGCGGGAGCGGAAGAGCTGGCGTTCCTCGTCTATGACAGCAACCTTGATTTCGCAGGCCAAGCCGGCATTTATAAAAACCAAGATAAAGGTTACCAGTACTTTGACTGGGACGGCACGATTAATGGCGGAACCAAACTTCCGGCCGGAGAGTATTACTTGCTCGCATATGCCGCGAACAAAGGCAAGTCAAGCCAGGTTTTGACCGAAGAACCTTTCACTGTTGAATAA
Reference Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed]
Miscellaneous -