Accession Number |
SPSED0017017 |
Secreted Protein |
Alkaline protease (alk) |
Uniprot Accession Number |
P41362 |
GenBank Accession Number |
AAC60420.1 |
Source Organism of the Secreted Protein |
Bacillus alcalophilus |
Nucleotide Sequence of the Secreted Protein |
Length = 1143 ntATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA |
Amino Acid Sequence of the Secreted Protein |
Length = 380 aaMKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR |
Expression Host |
Bacillus amyloliquefaciens 111018 |
|
Source Organism of the Signal Peptide |
Bacillus subtilis 168 |
Source Gene of the Signal Peptide |
yfkD |
Signal Peptide Sequence |
Length = 27 aaMMKKLFHSTLIVLLFFSFFGVQPIHAK |
Type |
Sec |
Yield |
12404.96 (U/mL) |
Performance (Current Yield / Highest Yield) |
0.63 |
Charge |
3 |
Charge (N-Region) |
2 |
Hydrophobicity |
0.97 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
2.34 |
Signal Peptide Original Protein Sequence |
MMKKLFHSTLIVLLFFSFFGVQPIHAKKQFKVPNSVASISKENTYPNASQDQPMLQPSKLAKELLDHSEVKIENPHLIKMLNESNISGTPLAVGYRATIFLGKWALGYESNETVANWEYKKINTNRADNRGGKETAEMHYAQEQQYRVKGGLTAKVPNAEDVKSMMMQKAMKKTNLPLAFETVIGAGTKRDQIYKVAPKKIGYLHAYAPAVNEKGKVTYGEVYLVLKGNKRKLVVKNVTSQGIGAWIPVQDHVTFGFQLSSLPR |
Signal Peptide Original DNA Sequence |
ATGATGAAAAAGCTATTTCATTCCACACTTATTGTGTTGTTATTCTTTAGTTTTTTCGGCGTTCAGCCCATCCACGCGAAAAAGCAGTTTAAGGTTCCTAATTCTGTCGCAAGCATTTCAAAGGAAAACACGTATCCGAATGCTTCACAGGATCAGCCAATGCTTCAGCCGAGCAAGCTGGCAAAGGAATTGCTCGATCATTCTGAGGTAAAGATTGAAAATCCGCATCTCATCAAAATGCTGAATGAATCCAACATATCCGGCACACCGCTTGCAGTAGGGTACCGGGCGACAATATTTCTCGGGAAATGGGCGCTCGGCTATGAATCAAATGAAACAGTCGCAAACTGGGAATATAAAAAAATCAATACAAACCGTGCGGATAACCGCGGCGGGAAAGAAACGGCTGAAATGCATTATGCCCAGGAACAGCAGTACAGAGTGAAGGGCGGCTTGACAGCTAAAGTTCCAAATGCTGAAGATGTCAAAAGCATGATGATGCAAAAAGCAATGAAGAAAACGAACCTCCCGCTTGCATTTGAGACGGTCATCGGGGCTGGCACAAAACGGGATCAGATTTATAAAGTCGCTCCTAAAAAAATCGGTTATCTACATGCATATGCACCGGCTGTGAATGAAAAAGGAAAAGTGACTTACGGAGAAGTGTACTTAGTGCTGAAAGGAAATAAACGAAAACTCGTCGTGAAAAATGTCACGTCACAGGGAATCGGCGCGTGGATTCCGGTTCAAGATCATGTCACGTTTGGCTTTCAGCTTTCCTCACTGCCAAGATAA |
Reference |
Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed] |
Miscellaneous |
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