SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0017020
| Accession Number | SPSED0017020 |
| Secreted Protein | Alkaline protease (alk) |
| Uniprot Accession Number | P41362 |
| GenBank Accession Number | AAC60420.1 |
| Source Organism of the Secreted Protein | Bacillus alcalophilus | Nucleotide Sequence of the Secreted Protein | Length = 1143 ntATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA |
| Amino Acid Sequence of the Secreted Protein | Length = 380 aaMKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR |
| Expression Host | Bacillus amyloliquefaciens 111018 |
| Source Organism of the Signal Peptide | Bacillus subtilis 168 |
| Source Gene of the Signal Peptide | hxlR |
| Signal Peptide Sequence | Length = 19 aaMSRMDDKRFNCEKELTLAV |
| Type | Tat |
| Yield | 12250 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.62 |
| Charge | 0 |
| Charge (N-Region) | 1 |
| Hydrophobicity | -0.69 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 1.9 |
| Signal Peptide Original Protein Sequence | MSRMDDKRFNCEKELTLAVIGGKWKMLILWHLGKEGTKRFNELKTLIPDITQKILVNQLRELEQDMIVHREVYPVVPPKVEYSLTPHGESLMPILEAMYEWGKGYMELIDIDKNVMKESL |
| Signal Peptide Original DNA Sequence | TTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTCAATGAATTAAAAACATTGATTCCTGATATTACGCAGAAGATCCTCGTGAATCAGCTGAGAGAGCTTGAGCAGGATATGATTGTTCACAGGGAAGTGTATCCAGTTGTCCCGCCGAAGGTTGAATATTCTCTGACCCCGCACGGAGAAAGCCTCATGCCTATTCTTGAAGCCATGTATGAGTGGGGGAAAGGCTATATGGAATTGATTGATATCGACAAAAATGTCATGAAAGAATCGTTGTAA |
| Reference | Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed] |
| Miscellaneous | - |