SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0017021

Accession Number SPSED0017021
Secreted Protein Alkaline protease (alk)
Uniprot Accession Number P41362
GenBank Accession Number AAC60420.1
Source Organism of the Secreted Protein Bacillus alcalophilus
Nucleotide Sequence of the Secreted Protein Length = 1143 nt
ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA
Amino Acid Sequence of the Secreted Protein Length = 380 aa
MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR
Expression Host Bacillus amyloliquefaciens 111018

Source Organism of the Signal Peptide Bacillus subtilis strain 1A747
Source Gene of the Signal Peptide ykoJ
Signal Peptide Sequence Length = 26 aa
MLKKKWMVGLLAGCLAAGGFSYNAFA
Type Sec
Yield 12078.78 (U/mL)
Performance (Current Yield / Highest Yield) 0.61
Charge 3
Charge (N-Region) 3
Hydrophobicity 0.79
Hydrophobicity Plot
Hydrophobicity (H-region) 1.56
Signal Peptide Original Protein Sequence MLKKKWMVGLLAGCLAAGGFSYNAFATENNENRQASSKTDALTEQEAEAIAKTVVDGTVEDIDRDLYNGKEVYEVEIEKEGEDYDVYVDIHTKQALNDPLKEKAEQVAITKEEAEEIALKQTGGTVTESKLDEDDGAYIYEMEIQTKQGTETEFEISAKDGRIIKQEIDD
Signal Peptide Original DNA Sequence ATGCTCAAGAAAAAATGGATGGTCGGTCTTTTAGCAGGGTGCCTAGCGGCCGGAGGATTCAGCTACAACGCGTTTGCCACAGAAAACAATGAAAACAGACAGGCTTCTTCTAAGACGGATGCACTCACTGAACAAGAAGCAGAAGCAATTGCTAAAACAGTTGTTGACGGCACAGTAGAAGACATTGACAGAGACCTTTACAACGGCAAAGAAGTCTATGAAGTGGAGATTGAAAAAGAAGGTGAAGACTACGACGTTTATGTAGATATTCATACAAAACAAGCGCTTAATGACCCGTTAAAGGAGAAAGCAGAACAAGTCGCCATAACCAAAGAAGAAGCTGAAGAAATTGCTTTAAAGCAAACAGGTGGAACCGTAACAGAAAGTAAGCTTGACGAAGATGACGGTGCTTACATCTATGAAATGGAAATCCAAACGAAGCAAGGAACTGAGACAGAATTTGAAATTTCCGCAAAAGATGGACGCATTATCAAACAGGAGATAGATGACTAA
Reference Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed]
Miscellaneous -