SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0017029

Accession Number SPSED0017029
Secreted Protein Alkaline protease (alk)
Uniprot Accession Number P41362
GenBank Accession Number AAC60420.1
Source Organism of the Secreted Protein Bacillus alcalophilus
Nucleotide Sequence of the Secreted Protein Length = 1143 nt
ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA
Amino Acid Sequence of the Secreted Protein Length = 380 aa
MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR
Expression Host Bacillus amyloliquefaciens 111018

Source Organism of the Signal Peptide Bacillus subtilis 168
Source Gene of the Signal Peptide ykwD
Signal Peptide Sequence Length = 26 aa
MKKAFILSAAAAVGLFTFGGVQQASA
Type Sec
Yield 10919 (U/mL)
Performance (Current Yield / Highest Yield) 0.55
Charge 2
Charge (N-Region) 2
Hydrophobicity 0.97
Hydrophobicity Plot
Hydrophobicity (H-region) 1.96
Signal Peptide Original Protein Sequence MKKAFILSAAAAVGLFTFGGVQQASAKELSCQPVVTVKTGNTVQNMSLNDAVKKLHINTNIKTLNAANEKEMKQLLQKHAKQSNVKVQDVQKTETAKPAQKTTEKAAADQNTASKAPATAEKTNTTTSAPSSVSAYEKKVVELTNAERQKQGLKPLQIDETLSKSARAKSQDMKDKNYFDHQSPTYGSPFDMMKSFGISYKTAGENIAKGQKTPEEVVKAWMNSEGHRKNILNPNFTHIGVGYVESGSIWTQQFIGK
Signal Peptide Original DNA Sequence ATGAAGAAAGCATTTATTTTATCTGCTGCCGCTGCGGTTGGATTATTCACATTCGGGGGCGTACAGCAAGCATCAGCGAAAGAACTCTCCTGCCAGCCTGTGGTTACAGTAAAAACGGGGAACACAGTACAAAACATGTCACTTAACGATGCAGTGAAAAAACTGCATATTAACACAAATATCAAAACGTTAAACGCTGCAAACGAAAAAGAGATGAAGCAGCTTCTTCAAAAACATGCGAAGCAATCTAACGTGAAAGTTCAGGATGTTCAAAAGACAGAAACGGCAAAACCTGCACAAAAGACTACTGAAAAAGCAGCTGCTGATCAAAACACTGCTTCTAAAGCACCCGCAACTGCTGAAAAAACAAACACAACAACGTCTGCACCTTCATCTGTAAGTGCATATGAGAAAAAAGTTGTTGAACTTACAAATGCAGAAAGACAAAAACAAGGCTTAAAACCGCTTCAAATTGATGAAACGTTAAGCAAATCTGCACGCGCAAAATCTCAGGATATGAAAGACAAAAACTACTTTGATCACCAAAGCCCAACATACGGCTCACCGTTTGATATGATGAAATCTTTCGGAATCAGCTACAAAACAGCAGGTGAAAACATTGCGAAGGGCCAAAAGACACCAGAAGAAGTAGTAAAAGCTTGGATGAACAGTGAAGGACACAGAAAAAACATTCTAAACCCTAACTTTACTCACATCGGTGTTGGTTACGTTGAGTCTGGAAGCATCTGGACACAACAATTCATCGGAAAATAA
Reference Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed]
Miscellaneous -