SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0018002

Accession Number SPSED0018002
Secreted Protein α-amylase (AmyS)
Uniprot Accession Number P06279
GenBank Accession Number AAA22235.2
Source Organism of the Secreted Protein Geobacillus stearothermophilus
Nucleotide Sequence of the Secreted Protein Length = 1650 nt
GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA
Amino Acid Sequence of the Secreted Protein Length = 549 aa
MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP
Expression Host Bacillus subtilis 1A751p

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide yqxI
Signal Peptide Sequence Length = 28 aa
MFKKLLLATSALTFSLSLVLPLDGHAKA
Type Sec
Yield 1394.15 (U/mL)
Performance (Current Yield / Highest Yield) 0.94
Charge 2
Charge (N-Region) 2
Hydrophobicity 0.9
Hydrophobicity Plot
Hydrophobicity (H-region) 1.87
Signal Peptide Original Protein Sequence MFKKLLLATSALTFSLSLVLPLDGHAKAQEVTLQAQQEVTYQTPVKLSELPTNTTEQSGEFHTNGIKKWIAKEAMKATASALRHGGRIVGEVVDELGGSAGKTFAKHTDDVADALDELVKRGDVVEDAIIDTVSSYLIDAGVKSSTARTIASVFTFLAF
Signal Peptide Original DNA Sequence ATGTTTAAGAAATTACTTTTAGCAACATCTGCATTAACATTCTCTTTATCATTAGTTCTTCCGTTGGATGGACATGCCAAAGCTCAAGAGGTAACATTACAGGCACAGCAGGAAGTCACTTATCAAACTCCCGTTAAACTATCCGAATTACCAACTAATACAACTGAACAATCTGGTGAATTTCACACAAATGGTATTAAAAAATGGATTGCTAAAGAAGCAATGAAGGCAACAGCCAGTGCGCTTCGACATGGTGGGAGAATTGTTGGTGAGGTAGTCGATGAGTTAGGTGGATCAGCAGGAAAAACATTCGCAAAACATACTGATGATGTAGCAGATGCTTTGGACGAGTTAGTTAAACGAGGTGACGTTGTGGAGGACGCAATTATTGACACGGTTTCCTCATATCTAATTGATGCTGGTGTAAAATCTTCTACAGCTAGAACTATTGCAAGTGTATTTACTTTCTTAGCTTTTTAA
Reference Fu G, Liu J L, Li J S, et al. Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2018, 66(50): 13141-13151. [PubMed]
Miscellaneous -