Accession Number |
SPSED0018012 |
Secreted Protein |
α-amylase (AmyS) |
Uniprot Accession Number |
P06279 |
GenBank Accession Number |
AAA22235.2 |
Source Organism of the Secreted Protein |
Geobacillus stearothermophilus |
Nucleotide Sequence of the Secreted Protein |
Length = 1650 ntGTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA |
Amino Acid Sequence of the Secreted Protein |
Length = 549 aaMLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP |
Expression Host |
Bacillus subtilis 1A751p |
|
Source Organism of the Signal Peptide |
Bacillus subtilis |
Source Gene of the Signal Peptide |
cwlO |
Signal Peptide Sequence |
Length = 30 aaMRKSLITLGLASVIGTSSFLIPFTSKTASA |
Type |
Sec |
Yield |
1064.05 (U/mL) |
Performance (Current Yield / Highest Yield) |
0.72 |
Charge |
3 |
Charge (N-Region) |
2 |
Hydrophobicity |
0.78 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
1.94 |
Signal Peptide Original Protein Sequence |
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTELQENQSKIEKELKDINDKALDTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEILKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSIVDADKDLIKQQEQDKAKLEDSEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASELANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDSSDNSSDNSSNGSSNSSSNGSSSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYKFGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTTDTLVGRGQAVSASEMKRGDLVFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ |
Signal Peptide Original DNA Sequence |
GTGAGAAAGAGTTTAATTACACTTGGTTTGGCTTCCGTCATCGGGACAAGCAGTTTTTTGATCCCATTTACAAGTAAAACTGCATCGGCGGAAACATTAGATGAAAAGAAACAAAAAATCGAAAGCAAGCAATCTGAGGTTGCTTCCAGCATTGAAGCGAAGGAAAAAGAATTAACCGAGCTTCAGGAAAATCAATCAAAGATTGAAAAAGAACTGAAAGACATTAACGATAAGGCGCTTGATACAAGCAACAAGATTGAAGATAAAAAAGAAGAAAACGATAAAACAAAAGAAGAAATCAAAAAACTGAAAAAAGAGATTAAAGAAACTGAAGCACGCATCGAAAAACGCAATGAAATCCTGAAAAAACGCGTTCGTTCTTTACAGGAAAGCGGCGGATCTCAAGGATACATAGATGTTCTTTTAGGATCAACAAGCTTTGGTGACTTTATCTCTCGTGCGACTGCGGTTTCATCTATTGTGGACGCAGACAAAGATTTAATCAAACAGCAAGAGCAGGATAAAGCGAAGCTCGAAGATTCTGAAGCGGATTTGAATGACAAGCTGAAAGAAGTTCAAGCTGCCTTGGCTAAATTAGAAACAATGCAAAAAGACCTTGATAAACAGCTTAATGAAAAAGACAAGCTTTTCGACGAAGCAAAAGCAAGCCAAAAGAAAACGGCTAAAGCGATTTCTGAATTAAAATCAGAGGCGTCTGAACTTGCAAATCAAAAAGCAAATACTGAAGCTGAACAAGCACGCATCAAAAAAGAACAAGAAGCAGCGGCTGCTTTGATCAAAAAGCAGGAAGAAGCACAAAAAGCATCTGATGAAACACAAACAGATGACAGTCAAACGGCGACAACTGAATCATCAAAAGCAAGCTCATCTGATGATTCTTCAGACAATTCTTCAGACAATTCTTCTAACGGTTCATCAAACAGTTCGTCAAACGGCTCATCTTCTAAGAAGAGCAGCGGCTCAAACAGCAATTCAGGCGGCACTGTTATCAGCAACTCTGGCGGAATTGAAGGCGCGATCAGCGTTGGTTCAAGCATTGTAGGACAATCTCCGTACAAGTTTGGCGGCGGACGCACTCAGTCTGATATCAACAACCGTATTTTTGACTGCTCATCATTCGTACGCTGGGCATACGCTTCTGCAGGTGTTAACCTTGGACCTGTAGGCGGAACAACAACTGATACGTTAGTTGGCAGAGGACAAGCTGTCAGCGCGTCTGAAATGAAACGCGGAGACCTTGTGTTCTTTGACACTTACAAAACAAATGGACACGTAGGAATCTACTTAGGAAACGGTACTTTCCTAAACGACAATACATCTCATGGCGTATCTGTTGACTCTATGAGCAATCCTTACTGGAAAGCAGCATTTAAAGGTGTTGTAAGACGTGTTGTTCAATAA |
Reference |
Fu G, Liu J L, Li J S, et al. Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2018, 66(50): 13141-13151. [PubMed] |
Miscellaneous |
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