Detail Information for SPSED0019001

Accession Number SPSED0019001
Secreted Protein Lipase A
Uniprot Accession Number P37957
GenBank Accession Number CAB12064.1
Source Organism of the Secreted Protein Bacillus subtilis 168
Nucleotide Sequence of the Secreted Protein Length = 639 nt
ATGAAATTTGTAAAAAGAAGGATCATTGCACTTGTAACAATTTTGATGCTGTCTGTTACATCGCTGTTTGCGTTGCAGCCGTCAGCAAAAGCCGCTGAACACAATCCAGTCGTTATGGTTCACGGTATTGGAGGGGCATCATTCAATTTTGCGGGAATTAAGAGCTATCTCGTATCTCAGGGCTGGTCGCGGGACAAGCTGTATGCAGTTGATTTTTGGGACAAGACAGGCACAAATTATAACAATGGACCGGTATTATCACGATTTGTGCAAAAGGTTTTAGATGAAACGGGTGCGAAAAAAGTGGATATTGTCGCTCACAGCATGGGGGGCGCGAACACACTTTACTACATAAAAAATCTGGACGGCGGAAATAAAGTTGCAAACGTCGTGACGCTTGGCGGCGCGAACCGTTTGACGACAGGCAAGGCGCTTCCGGGAACAGATCCAAATCAAAAGATTTTATACACATCCATTTACAGCAGTGCCGATATGATTGTCATGAATTACTTATCAAGATTAGATGGTGCTAGAAACGTTCAAATCCATGGCGTTGGACACATCGGCCTTCTGTACAGCAGCCAAGTCAACAGCCTGATTAAAGAAGGGCTGAACGGCGGGGGCCAGAATACGAATTAA
Amino Acid Sequence of the Secreted Protein Length = 212 aa
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide LipA
Signal Peptide Sequence Length = 31 aa
MKFVKRRIIALVTILMLSVTSLFALQPSAKA
Type Sec
Yield 7.64 (U/mL)
Performance (Current Yield / Highest Yield) 1
Charge 5
Charge (N-Region) 4
Hydrophobicity 1.04
Hydrophobicity Plot
Hydrophobicity (H-region) 2.57
Signal Peptide Original Protein Sequence -
Signal Peptide Original DNA Sequence -
Reference LingLi Zeng.Effects of different signal peptides on the secretion of lipase A in Bacillus subtilis[D]. Jiangnan University, 2009. (in Chinese)
Miscellaneous -