SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0019003
| Accession Number | SPSED0019003 |
| Secreted Protein | Lipase A |
| Uniprot Accession Number | P37957 |
| GenBank Accession Number | CAB12064.1 |
| Source Organism of the Secreted Protein | Bacillus subtilis 168 | Nucleotide Sequence of the Secreted Protein | Length = 639 ntATGAAATTTGTAAAAAGAAGGATCATTGCACTTGTAACAATTTTGATGCTGTCTGTTACATCGCTGTTTGCGTTGCAGCCGTCAGCAAAAGCCGCTGAACACAATCCAGTCGTTATGGTTCACGGTATTGGAGGGGCATCATTCAATTTTGCGGGAATTAAGAGCTATCTCGTATCTCAGGGCTGGTCGCGGGACAAGCTGTATGCAGTTGATTTTTGGGACAAGACAGGCACAAATTATAACAATGGACCGGTATTATCACGATTTGTGCAAAAGGTTTTAGATGAAACGGGTGCGAAAAAAGTGGATATTGTCGCTCACAGCATGGGGGGCGCGAACACACTTTACTACATAAAAAATCTGGACGGCGGAAATAAAGTTGCAAACGTCGTGACGCTTGGCGGCGCGAACCGTTTGACGACAGGCAAGGCGCTTCCGGGAACAGATCCAAATCAAAAGATTTTATACACATCCATTTACAGCAGTGCCGATATGATTGTCATGAATTACTTATCAAGATTAGATGGTGCTAGAAACGTTCAAATCCATGGCGTTGGACACATCGGCCTTCTGTACAGCAGCCAAGTCAACAGCCTGATTAAAGAAGGGCTGAACGGCGGGGGCCAGAATACGAATTAA |
| Amino Acid Sequence of the Secreted Protein | Length = 212 aaMKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN |
| Expression Host | Bacillus subtilis WB600 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | wprA |
| Signal Peptide Sequence | Length = 31 aaMKRRKFSSVVAAVLIFALIFSLFSPGTKAAA |
| Type | Tat |
| Yield | 0.77 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.1 |
| Charge | 5 |
| Charge (N-Region) | 4 |
| Hydrophobicity | 0.98 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 2.44 |
| Signal Peptide Original Protein Sequence | MKRRKFSSVVAAVLIFALIFSLFSPGTKAAAAGAIDQAAALENGKEQTGAMKEPEQVKWYKVTPGATDIQKNSHMALTVKSDSVLNVSVYPSKEKALKDETFEMYRSFTAEDGKSEVIFPYAWSGPYYVKVEYLGEEEPEDGGTAEAAAEAKYTIGYKGTKKQPSDLEEEEACPVEMSVDQKKSGKGILDKLRSIRDEQLSQTAEGKELTSLYYKAAPFIVAKLALNKTARNEIYQDLVTLKPLFDDVSENGASSSYKVTEKDQKAINRLYDKALQSVPSFLKEEIKKQADRLNMKQLQGKTAGAILTENNIAAKSEVQTTKVIFKVKDNKSLSSVHNEMKGFSASAQSKKDISNVKKAKKLFDNLYSFELPKDEKQNGAYTASAKRVKSAAATLSKMSNVEFAEPVQEYKSLANDIQYPYQWPLKNNGENGGVKNADVKYEPANTLLSKRKLNDTLIAVVDTGVDSTLADLKGKVRTDLGHNFVGRNNNAMDDQGHGTHVAGIIAAQSDNGYSMTGLNAKAKIIPVKVLDSAGSGDTEQIALGIKYAADKGAKVINLSLGGGYSRVLEFALKYAADKNVLIAAASGNDGENALSYPASSKYVMSVGATNRMDMTADFSNYGKGLDISAPGSDIPSLVPNGNVTYMSGTSMATPYAAAAAGLLFAQNPKLKRTEVEDMLKKTADDISFESVDGGEEELYDDYGDPIEIPKTPGVDWHSGYGRLNVMKAVSAADLQLKVNKLESTQTAVRGSAKEGTLIEVMNGKKKLGSAKAGKDNAFKVNIATQKQDQVLYLKATKGDAKTSYKVVVVKGKPSGTPKVNAVKTKDTAVKGKANSKAMIRVKNKSKKVIASAKADAKGTFSVKIKKQKAGTVLYVTAVDTDKKESKEAKVVVEK |
| Signal Peptide Original DNA Sequence | ATGAAACGCAGAAAATTCAGCTCGGTTGTGGCGGCAGTGCTTATTTTTGCACTGATTTTCAGCCTTTTTTCTCCGGGAACCAAAGCTGCAGCGGCCGGCGCGATCGATCAGGCGGCTGCTCTGGAAAACGGCAAAGAGCAGACAGGCGCCATGAAGGAGCCGGAACAGGTGAAATGGTACAAAGTGACCCCGGGAGCAACGGATATTCAGAAAAACTCACATATGGCACTGACCGTAAAGAGTGATTCAGTACTGAATGTATCTGTATATCCAAGTAAGGAAAAAGCGCTTAAAGATGAGACGTTTGAGATGTACCGTTCTTTCACAGCGGAGGATGGAAAAAGCGAAGTCATTTTTCCATACGCGTGGAGCGGCCCTTACTATGTAAAAGTTGAATACCTCGGAGAAGAAGAACCAGAGGACGGCGGAACGGCAGAAGCAGCTGCAGAAGCCAAGTATACGATTGGGTATAAAGGCACCAAAAAACAGCCGTCAGATTTAGAAGAGGAAGAAGCTTGTCCGGTTGAAATGAGTGTCGATCAGAAGAAATCAGGAAAAGGCATCCTGGATAAGTTGAGATCGATTCGTGATGAGCAGCTGAGCCAAACAGCAGAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATCTAATGTGAAAAAGGCAAAGAAACTGTTTGACAATCTGTATTCATTTGAACTTCCGAAAGACGAGAAACAGAACGGCGCATATACGGCAAGCGCCAAAAGGGTCAAAAGCGCTGCTGCGACACTATCCAAGATGTCCAATGTAGAGTTTGCGGAACCCGTACAGGAATACAAAAGCTTGGCAAACGATATTCAGTACCCTTATCAATGGCCGCTTAAAAACAACGGTGAAAACGGCGGTGTCAAAAATGCGGATGTGAAATATGAGCCTGCCAACACATTGCTGTCCAAACGCAAGCTTAACGATACACTCATTGCAGTAGTAGACACAGGCGTAGACAGCACGCTTGCCGATTTAAAAGGAAAAGTAAGAACAGATCTCGGACACAATTTTGTCGGACGAAATAACAATGCAATGGATGATCAGGGGCATGGGACGCATGTCGCAGGCATTATTGCAGCCCAAAGCGATAACGGCTATTCAATGACTGGATTGAATGCCAAAGCAAAAATCATCCCTGTAAAAGTGCTTGATTCCGCAGGTTCCGGAGATACTGAACAAATTGCTCTCGGCATCAAATATGCTGCTGACAAAGGAGCAAAGGTGATTAATTTAAGTTTAGGCGGAGGCTACAGCCGCGTGCTTGAATTTGCTTTGAAGTACGCAGCTGACAAAAATGTCTTGATTGCCGCAGCCAGCGGGAATGATGGAGAAAATGCCTTATCTTATCCTGCATCTTCTAAATATGTGATGTCAGTCGGCGCAACGAATCGCATGGATATGACCGCTGATTTCTCTAACTATGGAAAAGGTCTGGACATCTCTGCTCCAGGGTCTGATATCCCGAGCTTAGTGCCGAACGGAAATGTCACGTACATGAGCGGAACGTCTATGGCGACGCCATATGCTGCCGCCGCTGCGGGACTGCTGTTTGCTCAAAATCCTAAGCTAAAAAGAACAGAAGTTGAGGATATGTTGAAAAAGACGGCAGATGATATTTCCTTTGAAAGTGTCGATGGCGGAGAAGAAGAGTTGTATGACGATTATGGCGATCCGATTGAAATTCCGAAGACACCTGGTGTAGACTGGCATTCAGGCTACGGGCGGCTGAATGTCATGAAGGCTGTCAGCGCAGCTGATTTACAGCTTAAGGTCAACAAGCTGGAAAGCACTCAAACAGCTGTCAGAGGAAGCGCGAAGGAAGGCACCCTTATCGAGGTGATGAACGGCAAAAAGAAACTCGGCAGCGCCAAAGCCGGAAAAGACAATGCGTTCAAGGTGAATATCGCGACTCAAAAACAGGATCAAGTACTGTATCTGAAAGCAACAAAAGGCGATGCGAAAACATCGTATAAAGTTGTTGTCGTCAAAGGAAAACCTTCCGGCACACCGAAAGTAAACGCGGTGAAAACGAAGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAA |
| Reference | LingLi Zeng.Effects of different signal peptides on the secretion of lipase A in Bacillus subtilis[D]. Jiangnan University, 2009. (in Chinese) |
| Miscellaneous | - |