SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0019005

Accession Number SPSED0019005
Secreted Protein Lipase A
Uniprot Accession Number P37957
GenBank Accession Number CAB12064.1
Source Organism of the Secreted Protein Bacillus subtilis 168
Nucleotide Sequence of the Secreted Protein Length = 639 nt
ATGAAATTTGTAAAAAGAAGGATCATTGCACTTGTAACAATTTTGATGCTGTCTGTTACATCGCTGTTTGCGTTGCAGCCGTCAGCAAAAGCCGCTGAACACAATCCAGTCGTTATGGTTCACGGTATTGGAGGGGCATCATTCAATTTTGCGGGAATTAAGAGCTATCTCGTATCTCAGGGCTGGTCGCGGGACAAGCTGTATGCAGTTGATTTTTGGGACAAGACAGGCACAAATTATAACAATGGACCGGTATTATCACGATTTGTGCAAAAGGTTTTAGATGAAACGGGTGCGAAAAAAGTGGATATTGTCGCTCACAGCATGGGGGGCGCGAACACACTTTACTACATAAAAAATCTGGACGGCGGAAATAAAGTTGCAAACGTCGTGACGCTTGGCGGCGCGAACCGTTTGACGACAGGCAAGGCGCTTCCGGGAACAGATCCAAATCAAAAGATTTTATACACATCCATTTACAGCAGTGCCGATATGATTGTCATGAATTACTTATCAAGATTAGATGGTGCTAGAAACGTTCAAATCCATGGCGTTGGACACATCGGCCTTCTGTACAGCAGCCAAGTCAACAGCCTGATTAAAGAAGGGCTGAACGGCGGGGGCCAGAATACGAATTAA
Amino Acid Sequence of the Secreted Protein Length = 212 aa
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide wapA
Signal Peptide Sequence Length = 32 aa
MKKRKRRNFKRFIAAFLVLALMISLVPADVLA
Type -
Yield 0.18 (U/mL)
Performance (Current Yield / Highest Yield) 0.02
Charge 7
Charge (N-Region) 8
Hydrophobicity 0.59
Hydrophobicity Plot
Hydrophobicity (H-region) 2.69
Signal Peptide Original Protein Sequence MKKRKRRNFKRFIAAFLVLALMISLVPADVLAKSTEEENGNRIAADDPEETLQKEQTEEAVPFDPKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPDADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLAEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYNGYNTFTFQLKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKAYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMTVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNAKVTWNTKPASKNIGKADVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPTIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHLDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSAKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGSGAELPINPGPTYKNAGGDGAKRNYSFKIIAYNKDGEAIASPAATPALPDIARPKNVTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSFDVGDADHWTTQNKNIWPTSEEIKAGSYKLHTDGKGGELALDPSPVYNNANGNYKGKKNYSFTLVAYDANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDSSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYENDLLKKVTDTDGTVTSYDYDSEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHKDKDGKGKAWFDEVQLEKGEVSSSYNPVQNSSFTSATENWNVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQSANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGIYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGYATSTDYDETGKKTSETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKFEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYIFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWIHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGDILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTIKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGKVTNYFYDGDSLNVLYETDADNNVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDIIAISDSTGKTVAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGASRAYKFTKKAVKITGHTRHGLNQSIGRNGGRGVNLRAKLNAVRSPKKVIKQPNGATKYVGKKATVVLNKRGKVITAYGSSRAKGSKHVFHTHGKGNKSKRRR
Signal Peptide Original DNA Sequence ATGAAAAAAAGAAAGAGGCGAAACTTTAAAAGGTTCATTGCAGCATTTTTAGTGTTGGCTTTAATGATTTCATTAGTGCCAGCCGATGTACTAGCAAAATCTACAGAAGAAGAGAATGGAAACCGTATTGCTGCCGATGATCCAGAAGAAACTCTTCAGAAAGAACAAACTGAGGAAGCTGTACCTTTTGATCCAAAAGATATAAACAAAGAGGGTGAGATCACCTCTGAAAGAACAGAAAATACAAAACTCTATTATGAAGGTGACGGTGTCTATAAACAGGAAGTATACCTTGATCCTATTCATACAAAGGAAACACCGGATGCCGACTGGGAAGACATTTCTCCTGAGTTAAAAGAGTCGACAAGCAAACAAGTCGAGACAGAGAACGCTATTTTGAATTCAGATTTTCAAAAGCAAATGAAGAACGGGCTTTATGCGACTTTTGAGCATAATGACCATAAGGTTACATATTCACTAGCAGAAGCGAAAGGCCCGAATAAAACTTCACTTACTCCGAAAGATACCTCAGCAGATTATAAGACTGACAGTAATGAGATTGTGTATCCGGATGTGTTTCCGAATATTGATCTTCAGACTTTCACATTCAATGAAAACATAAAAGAAGATTTAGTCCTTCATCAGTATAATGGATACAACACATTTACATTTCAGTTAAAAACCGATTTGCAGGCTAAAGAACAAGAGGATGGATCCATTGATTTTTCTGATGAAAAAGGAAAAGTTGTTTTTTCTGTGCCTAAGCCTTTTATGACGGATTCAAAACTAGATGAGCTTTCCGGCGAGGTTGAACGGTCAGACAAAGTCAGCTATAAGCTGGAAAAGAATGAAGAAGGGTATTTGCTTCATCTTACGGCAGACGAGAACTGGCTGAAAGACCCAGAACGCGTATATCCAGTATCTATCGACCCGTCAACTTCTTTATCTGTTTCATCGGATACGTTTGTCATGAGTGCCTACCCGACGACGAACTATTCGGCCTCCTCACAAAAATGGGATGCTAATTTAAAGGCCTATGTACTGAAAACGGGCTATTATGACAAAACAACAGGCACAAACTATGCGTTCATGAAATTTAACAACCTAAAGCCAATTCAAAATATGACTGTCACTAAAGCTACATTAAAAACATACGTAGCCCATTCCTATTATGGAACAAAGGCAACCGGCCTTTGGCTGGATACGGTCAACAGCAATTATGATAATGCAAAAGTGACATGGAACACCAAACCGGCATCTAAAAATATCGGAAAAGCCGACGTCCATAAAGGACAGTGGGCGAGTTATGATGTGACCGCTGCTGTAAAGTCATGGAACTCCGGCGGGGCCAACTATGGATTTAAGCTTCATACAAACGGAAACGGCAAGGAATATTGGAAAAAACTAATTTCTTCAGCGAACTCCGCCAATAAACCATATATTGAAGTGACGTATACCATTCCAAAGGGAAACACACCTACAATCAAAGCCTATCACAACGGCGATTCCACTGGTTATTTCGATATCAGCTGGAAAAAGGTTGAAGGCGCAAAAGGATATAAGGTATGGATTTACAATGGTAAGGAATATCAAGCGATTTCTGCAGGCAACGTCACGTCGTGGAGCACAAAAGGTAAAAAAATCTGGCCTACGAGCGCAGAAATTGCCTCCAAGAGATATAAACTGCATCTGGACGGCAAGGATGGAGCTGAATTGGCATTAGATCCATCCCCGGTCTATAAGAATTCCGGCGGAAGCTATGCTACAAGCAAAAACTATTGGATTGGCGTCAGTGCGATATTTGACCAAGGTGAAGGAGCGATGTCCGCACCGGCAAAGCCCGTTATCCCTAATGTAGGAAAAGCACAAGCACCGTCAGCAAAGGGCTACAATAATGGGAATGCCACAGGATACTTCGACCTTTCTTGGAAAGCTGTATCCGGTGCAACCGGCTATAAGGTTCAGGTGTTCAATGGGAAAGGCTTTGAGACACTTGATCTCGGAAATCAGACGTCTTGGACCACAAAAGGGAAAAAGATCTGGCCGACAAGTGCAGAAATCAAGGCGGGAAAATATGCTCTTCACCTAAAGGACGGAAGCGGAGCAGAGCTGCCGATCAATCCCGGACCAACCTATAAAAACGCCGGGGGAGATGGAGCCAAAAGAAATTACTCGTTTAAAATTATCGCATACAACAAAGACGGCGAAGCCATTGCATCCCCGGCTGCTACCCCGGCACTTCCTGATATCGCTAGGCCAAAGAATGTAACTGGCTATTTGTATACAAATACAAAATCGAGTCAAACGGGTTATGTGAATTTGATATGGGAAAAGGTTCAAAATGCGAAGGGCTATAAAGTTAACATTTACAACGGTAAAGAATATCAGTCCTTTGATGTTGGTGATGCTGATCATTGGACAACCCAAAATAAAAACATCTGGCCGACTTCTGAGGAAATCAAAGCCGGAAGCTATAAGCTTCATACAGATGGAAAAGGCGGGGAATTAGCCCTCGATCCATCACCTGTCTATAACAATGCAAATGGGAATTACAAAGGGAAGAAGAATTATTCTTTCACGCTTGTCGCCTATGATGCGAATGGTGAAACGATTCCAACGGCGCCCTTTAACCCAACGTTCCATGAAGGCGCAGAATTCCTAGGTACAGAAGAGTACTGGTCTATCATTGATATCCCAAGCGGCCAGTTAAATGGTGCGACAGGCAATGTCATTGTGAATGAAGAAGATTTGTCAATTGATGGACGCGGTCCGGGACTTGGCTTATCGAGAACCTACAACAGTCTTGACAGCTCTGATCACTTATTTGGACAAGGCTGGTATGCTGATGCAGAAACATCTGTCATCTCAACGGATGGCGGGGCAATGTATATCGATGAAGACGCTACGACACATCGATTCACGAAAAAAGCCGATGGCACGTATCAGCCGCCGACCGGTGTATATCTTGAATTAACAGAAACAGCTGATCAGTTCATATTAAAAACAAAGGATCAAACCAACGCTTATTTCAATAAAAAAGGCGGCAAACTCCAAAAGGTAGTGGATGGCCATAACAATGCAACTGTCTATACTTACAATGACAAAAATCAACTGACCGCCATTACAGATGCATCAGGCCGCAAGCTTACATTTACCTATGATGAAAACGGCCATGTGACGTCCATCACAGGACCTAAGAATAAGAAAGTCACCTACTCATATGAGAATGATTTGCTGAAGAAGGTTACAGATACAGACGGGACTGTCACAAGCTATGACTACGATAGTGAAGGGCGCCTTGTCAAACAATATTCAGCTAACAGTACCGAAGCAAAGCCTGTCTTTACCGAATATCAGTATAGCGGTCACCGTCTCGAAAAAGCGATCAACGCTAAAAAAGAAACATATGTCTACAGCTATGATGCTGATAAAAAGACGCTTCTCATGACACAGCCAAATGGCCGGAAAGTACAGTATGGCTATAATGAAGCGGGAAATCCGATTCAGGTCATTGATGATGCGGAAGGGTTAAAAATTACAACGAATACAAAGTATGAAGGCAACAATGTTGTAGAAGATGTAGATCCAAATGACGTCGGCACCGGAAAAGCAACGGAAAGCTATCAATATGACAAAGATGGCAACGTTACGTCGGTTAAAGATGCATATGGAACTGAGACATACGAATACAACAAAAACAACGATGTCACAAAGATGAAGGATACCGAGGGCAATGTCACGGATATTGCTTATGACGGACTGGATGCTGTATCCGAAACCGATCAGAGCGGAAAATCATCGTCAGCAGCTGTTTATGATAAATATGGAAACCAGATTCAATCGAGCAAAGATTTATCAGCTTCAACTAATATTTTGAAAGATGGAAGCTTTGAAGCTCAAAAATCCGGCTGGAATCTGACTGCCTCTAAAGACAGCGGCAAAATTTCAGTCATCGCCGATAAAAGCGGAGTTCTTTCCGGATCAAAGGCATTGGAGGTCTTGAGCCAATCAACGTCTGCCGGAACAGATCATGGGTATTCGTCAGCAACACAAACAGTTGAGCTGGAGCCGAATACAACCTATACATTAAGCGGGAAAATCAAAACAGATCTTGCGAAATCAAGAGCCTATTTTAACATTGATTTGCGAGACAAAGATCAAAAACGAATTCAATGGATTCACAATGAATACAGTGCATTAGCCGGAAAAAATGACTGGACAAAACGGCAAATCACTTTTACGACTCCAGCGAATGCAGGCAAAGCTGTTGTGTATATGGAAGTCGATCATAAAGATAAAGATGGCAAAGGAAAAGCATGGTTTGATGAAGTCCAGCTGGAAAAAGGCGAAGTATCATCAAGCTATAACCCAGTTCAAAACAGCAGCTTCACATCAGCAACGGAAAACTGGAATGTAAGCGGAGCATCCGTTGATTCTGAAGAAGGCTTTAATGATGATGTATCATTAAAAGCTGCCCGGACCAGTGCGTCCCAAGCCGGATCAGTAACAAAACAAACCGTTGTCTTAGGGCAAAGCGCAAATGACAAACCGGTTTATCTCACACTGACAGGAATGTCGAAAGCAAGCAGCGTCAAATTTACAGATGAAAAAGACTATTCTCTGCAGGCGAATGTCACTTACGCTGACGGCAGCACAGGCATCTACAATGCGAAATTCCCTTCTGGAACACAGGAATGGAACCGAGCAGCTGTCGTCATCCCTAAAACAAAGCCGATCAATAAAGTCGATATCAGTATTCTGTTCCAAAAGAGTGCGACAGGCACAGTCTGGTTTGATGATATCCGTTTGATTGAAGGAAGCCTTCTGACTAAAAGCACGTATGACAGTAATGGCAACTATGTGACCAAGGAAGAGGACGAACTAGGTTACGCCACTTCAACTGATTACGATGAAACTGGTAAGAAAACGTCTGAAACAGATGCAAAAGGTGAAAAAACAACCTATACGTATGATCAGGCAGACCAATTGACAAACATGACGCTCTCTAACGGCACGTCTATCCTTCACAGCTATGATAAGGAAGGCAATGAGGTAAGCAAAACCATCCGGGCAGGAGCAGATCAAACCTATAAATTCGAATATGATGTCATGGGCAAGCTTGTCAAAACAACTGACCCTCTCGGCAATGTGTTAGCAAGCGAATATGATGCCAACAGCAACCTGACCAAAACCATTTCACCAAATGGCAACGAAGTGTCTCTTTCTTATGATGGAACAGACCGGGTGAAATCAAAGTCGTATAACGGTACAGAAAAGTACATTTTCACGTATGACAAAAATGGAAATGAAACGTCGGTCGTCAATAAAGAGCAGAACACCACAAAGAAACGGACATTTGACAACAAAAACCGTTTAACCGAACTGACAGACCGCGGCGGCAGCCAAACTTGGACATATCCTAGTGACTCCGATAAGCTGAAAACCTTCTCGTGGATTCATGGGGACCAAAAAGGAACGAATCAATTTACGTATAACAAGCTGGATCAAATGATTGAGATGAAAGACAGTACATCCTCATATTCATTTGATTATGATGAAAATGGAAACGTCCAAACCTTTATTACGGGCAACGGCGGCGGAACAAGTTTTTCATATGACGAAAGAAACCTTGTAAGCTCGCTGCATATCGGTGACAAAAACGGCGGAGACATTCTGACGGAAAGCTATGAATATGATGCCAACGGCAACCGCACAACGATAAACAGCTCAGCAAGCGGCAAGGTGCAATATGAGTATGGGAAGCTGAATCAGCTTGTCAAAGAAACCCATGAAGACGGTACTGTTATTGAATACACATATGATGGATTCGGAAACCGGAAAACCGTTACAACTATAAAAGACGGCTCAAGCAAAACGGTGAACGCTTCCTTCAACATCATGAACCAGCTGACAAAAGTCAATGATGAAAGCATCTCTTACGATAAAAACGGCAACCGCACATCCGACGGCAAATTCACATATACGTGGGATGCAGAAGATAACCTGACCGCCGTTACCAAAAAAGGCGAAGACAAGCCATTCGCAACATATAAGTATGACGAAAAAGGAAACAGAATCCAAAAAACCGTCAACGGAAAAGTCACGAACTACTTCTACGACGGAGACAGCTTGAACGTCTTGTATGAAACAGACGCGGACAACAACGTCACAAAATCATACACGTACGGAGACAGCGGCCAGCTGTTATCCTACACAGAAAATGGTAAGAAATATTTCTATCACTACAACGCTCACGGCGACATCATCGCGATCTCAGACAGCACTGGAAAAACCGTTGCCAAGTATCAATACGACGCATGGGGAAATCCAACAAAAACCGAAGCAAGCGACGAAGTAAAAGACAATCGCTACCGCTACGCAGGCTACCAATACGACGAAGAAACCGGCCTGTACTATCTCATGGCCCGCTACTACGAGCCAAGAAACGGCGTGTTCCTATCGCTCGACCCAGACCCGGGCAGCGACGGAGATTCATTGGATCAGAATGGGTATGCTTATGGAAATAATAACCCAGTGATGAATGTTGATCCGGACGGGCATTGGGTATGGCTTGTGGTAAATGCCGGATTTGCAGCCTATGATGGATATAAGGCATATAAATCAGGTAAAGGATGGAAAGGTGCTGCTTGGGCAGCTGCTTCTAATTTTGGTCCTGGGAAGATATTTAAGGGTGCTAGTCGCGCCTATAAGTTTACGAAGAAGGCAGTTAAAATCACAGGTCATACAAGACATGGACTTAATCAATCTATAGGAAGAAATGGAGGCCGTGGAGTCAATTTAAGGGCCAAACTGAATGCTGTGCGAAGCCCTAAAAAAGTAATAAAACAACCTAATGGGGCAACGAAATATGTTGGAAAAAAAGCTACAGTTGTTTTAAATAAAAGGGGTAAAGTAATCACAGCTTATGGAAGTTCAAGAGCTAAGGGTTCTAAACACGTATTCCATACACACGGAAAAGGAAATAAATCTAAGAGAAGGCGTTAA
Reference LingLi Zeng.Effects of different signal peptides on the secretion of lipase A in Bacillus subtilis[D]. Jiangnan University, 2009. (in Chinese)
Miscellaneous -