SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0020005

Accession Number SPSED0020005
Secreted Protein Natto phytase
Uniprot Accession Number A0A0H5B9W2
GenBank Accession Number BAR91210.1
Source Organism of the Secreted Protein Bacillus subtilis natto starter
Nucleotide Sequence of the Secreted Protein Length = 1363 nt
AACAACAAAACTAAATAAACAATGTACTAAATATTCAACATATTTTTTTTTCGCTCCGCAAAAGCAAATCTGGCAAAGTCATCTCACGTACCTCTTTGGGGAATAAGTTGTTTATGAGTCAGAAACCCTATAAAAAAAGGTTCATTTTCCTCACGGTAATTACCTGTATATATTTTATAATAGTAGTGTTAGTGATAAAAGAGGAGGGTACCAAATGAAGGTTTCAAAAACAATGCTGCTAAGCACTGCCGCGGGTTTATTGCTTAGCCTGACAGCAACCTCGGTGTCGGCTCATTATGTGAATGAGGAACATCATTTCAAAGTGACTGCACACACGGAGACAGATCCGGTCGCATCTGGCGATGACGCAGCAGATGACCCGGCCATTTGGGTTCATGAAAAACACCCGGAAAAAAGCAAGTTGATTACAACAAATAAGAAGTCAGGGCTCGTCGTGTATGATTTAGACGGAAAACAGCTTCATTCTTATGAGTTTGGCAAGCTCAATAATGTCGATCTGCGCTATGATTTTCCATTGAACGGCGAAAAAATTGATATTGCTGCCGCATCCAATCGGTCCGAAGGAAAAAATACAATTGAAGTATATGCAATAGACGGGGATAAAGGAAAATTGAAAAGCATTACTGATCCGAACCATCCTATTTCCACCAATATTTCTGAGGTTTATGGATTCAGCTTGTATCACAGCCAGAAAACAGGAGCATTTTACGCATTAGTGACAGGCAAACAAGGGGAATTTGAGCAGTATGAAATTGTTGATGGTGGAAAGGGTTATGTAACAGGGAAAAAAGTGCGTGAATTTAAGTTGAATTCTCAGACCGAAGGCCTTGTTGCGGATGATGAGTACGGAAACCTATACATAGCAGAGGAAGATGAGGCCATCTGGAAATTTAACGCTGAGCCCGGCGGAGGATCAAAGGGGCAGGTTGTTGACCGTGCGACAGGAGATCATTTGACAGCTGATATTGAAGGACTGACAATCTATTATGCACCAAATGGCAAAGGATATCTCATGGCTTCAAGTCAAGGAAATAACAGCTATGCAATGTATGAACGGCAGGGGGAAAATCGCTATGTAGCCAACTTTGAGATTACAGATGGCGAGAAGATAGACGGTACTAGTGACACGGATGGTATTGATGTTCTCGGTTTCGGACTTGGCCCAAAATATCCGTACGGGATTTTTGTGGCGCAGGATGGCGAAAATATCGATAACGGACAAGCCGTCAATCAAAATTTCAAAATTGTATCGTGGGAACAAATTGCACAGCATCTCGGCGAAATGCCTGATCTTCATAAACAGGTGAACCCGAGGAAGCTGAAAGACCGTTCTGACGGCTAG
Amino Acid Sequence of the Secreted Protein Length = 382 aa
MKVSKTMLLSTAAGLLLSLTATSVSAHYVNEEHHFKVTAHTETDPVASGDDAADDPAIWVHEKHPEKSKLITTNKKSGLVVYDLDGKQLHSYEFGKLNNVDLRYDFPLNGEKIDIAAASNRSEGKNTIEVYAIDGDKGKLKSITDPNHPISTNISEVYGFSLYHSQKTGAFYALVTGKQGEFEQYEIVDGGKGYVTGKKVREFKLNSQTEGLVADDEYGNLYIAEEDEAIWKFNAEPGGGSKGQVVDRATGDHLTADIEGLTIYYAPNGKGYLMASSQGNNSYAMYERQGENRYVANFEITDGEKIDGTSDTDGIDVLGFGLGPKYPYGIFVAQDGENIDNGQAVNQNFKIVSWEQIAQHLGEMPDLHKQVNPRKLKDRSDG
Expression Host Bacillus subtilis RIK1285

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide bslB
Signal Peptide Sequence Length = 30 aa
MLKRTSFVSSLFISSAVLLSILLPSGQAHA
Type Sec
Yield 3103 (μmol/L per min)
Performance (Current Yield / Highest Yield) 0.73
Charge 2
Charge (N-Region) 2
Hydrophobicity 0.99
Hydrophobicity Plot
Hydrophobicity (H-region) 1.84
Signal Peptide Original Protein Sequence MLKRTSFVSSLFISSAVLLSILLPSGQAHAQSASIEAKTVNSTKEWTISDIEVTYKPNAVLSLGAVEFQFPDGFHATTRDSVNGRTLKETQILNDGKTVRLPLTLDLLGASEFDLVMVRKTLPRAGTYTIKGDVVNGLGIGSFYAETQLVIDPR
Signal Peptide Original DNA Sequence ATGCTAAAAAGAACTTCATTCGTATCTTCATTATTCATCAGTTCAGCTGTTTTACTATCAATCTTACTTCCTTCGGGCCAAGCTCATGCACAGTCTGCATCAATCGAGGCAAAAACAGTTAACAGCACGAAAGAGTGGACCATTTCTGATATTGAAGTGACATATAAACCAAATGCGGTGCTTTCTCTTGGAGCGGTAGAATTTCAATTTCCTGACGGGTTTCATGCTACGACAAGAGATTCAGTGAATGGAAGAACACTGAAAGAAACACAGATTTTAAACGATGGAAAAACAGTCAGACTCCCGCTTACGCTTGATTTGTTAGGCGCATCCGAATTTGACCTTGTCATGGTGCGTAAAACTCTTCCTCGCGCAGGCACTTACACGATTAAAGGCGATGTAGTAAACGGTTTGGGAATCGGCAGTTTTTATGCTGAAACGCAGCTGGTGATTGATCCCCGTTAA
Reference Tsuji S, Tanaka K, Takenaka S, et al. Enhanced secretion of natto phytase by Bacillus subtilis[J]. Bioscience Biotechnology and Biochemistry, 2015, 79(11): 1906-1914. [PubMed]
Miscellaneous -