SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0021002

Accession Number SPSED0021002
Secreted Protein Lipase (LipS)
Uniprot Accession Number S4TNY8
GenBank Accession Number AGF29555.2
Source Organism of the Secreted Protein Stenotrophomonas maltophilia
Nucleotide Sequence of the Secreted Protein Length = 1581 nt
ATGCGCAAGGTCCTCCTCCTCCTCATTCTCCTCATTGGCCTCGCCCCTACCGCGTGGGCGGCGATGGCGACGGTCAAGACCACCCATCGCACCATTGCCGGCTGGGATGGAACGCCTCTCGGGGCCTTCGTCATCGAACCGCAGGATGCGGGCGGCGGGCGCTATCCGCTGCTGGTGATGCCCAGCAGCTGGGCGGTGCCCAGCGTGGAGTACGTGGGGGTGGCGCAGTCGCTGGCACAGCGCGGCTATGTGGTGATCAGCTACAGCTCGCGTGGCTTCTGGGAATCCGGCGGCAGCATCGACATCGCCGGGCCCTCCACGGTGGAGGATGTCAGCGCGCTGATCGACTGGGCGCTGGACAACACCCGCGCTGACCCGGACCGGATCGGCGTTTCGGGCATCTCCTACGGTGCGGGCACGAGCCTGCTGGCGGCGGCACGCGATCCGCGCATCAAGGCCGTGGCCGCGCTCAGTGGCTGGGCCGATCTGCAGGCGTCGCTGTACAGCAACGACACCCCGAGCGCGCAGGGCATCGCACTGCTGGTTGCGGCCGGCCTGGTGACCGGACGCCCTGGCGCGGAGCTGGCCACGATCAACCGCAATGTTCTGGCGGGCAACTACCAGGGCGCGGTGGATTCGCTGTTGCCGGTGGCGGCGCAGCGCAGTCCCGCGGCCAGCATCGACGAGATCAACGCCAACCAGCCGGCGGTGTTCCTGGCCAACGCCTTCAACGACAGCCTGTTCCCGCCGGGCCAGCTGGTTGATTTCTTCAACCGGCTGAAAGGCCCGAAGCAGCTGCAGCTGCGCCACGGTGACCACGCGCTCAACGAAGCGCTCGGTGCACTGGGCATTCCCAACGAAGTCTATGACCAGGTGGGTGACTGGTTCGATCACTATCTGAAGGCAGTGGCCAACGGCATCGACCGCCAGCCGGCGGTGCAGCTGAAGTCACAGAAGGGCAGCTGGAGCAGTTACCCGGACTGGCAGGCGACCAGCAAGGGTGCGGTCAGCTACGGCCTGACCGCACCCTCTGGTTTGCTGCTGCCGACCGGCGGCCTGGCCGAGCACGGTGGCGGCACCGGCTGGAACTACCGCATCGGCAGCGGTTTGCTGACTGCGGCCAATTCCGGCGTGGCGATGGCCTCTGGCGCGCTGCAGATGATCAACCTGCCGCCGGGTGCCTACGTACCGTTTGTTGGCCGCAGTGCCGCCGGTGTCTGGCAGGGGCCGATCCAGTGGAGCGCCAAACGCCTGGACGGTGCGCCGGAAGTGCGCCTGACGGTCACGCCCAGCCGTGCCAACACCACGCTGTACGCCTACCTGTACGCCGAGGATGTGCTGGGCAATGGTCAGTTGATCAGCCACAAGCCGTACACCTTGCGCGGTGCCACGCCGGGCCAGGCGAAAACGCTCGACCTGCGGCTGGAAGCGAGCAGCTGGAACCTTCCAGCCGGCAGCCGCCTGACCTTGGTGGTCGATACCGTGGACCTGCGCTATGCGGGCATCAGCCAGCTGGGTGGGGCGGTGACCTTCACCTCGCCAGCCAATGCGCCGTCGGTGCTGAAAGTGCCGCTGCATTGA
Amino Acid Sequence of the Secreted Protein Length = 526 aa
MRKVLLLLILLIGLAPTAWAAMATVKTTHRTIAGWDGTPLGAFVIEPQDAGGGRYPLLVMPSSWAVPSVEYVGVAQSLAQRGYVVISYSSRGFWESGGSIDIAGPSTVEDVSALIDWALDNTRADPDRIGVSGISYGAGTSLLAAARDPRIKAVAALSGWADLQASLYSNDTPSAQGIALLVAAGLVTGRPGAELATINRNVLAGNYQGAVDSLLPVAAQRSPAASIDEINANQPAVFLANAFNDSLFPPGQLVDFFNRLKGPKQLQLRHGDHALNEALGALGIPNEVYDQVGDWFDHYLKAVANGIDRQPAVQLKSQKGSWSSYPDWQATSKGAVSYGLTAPSGLLLPTGGLAEHGGGTGWNYRIGSGLLTAANSGVAMASGALQMINLPPGAYVPFVGRSAAGVWQGPIQWSAKRLDGAPEVRLTVTPSRANTTLYAYLYAEDVLGNGQLISHKPYTLRGATPGQAKTLDLRLEASSWNLPAGSRLTLVVDTVDLRYAGISQLGGAVTFTSPANAPSVLKVPLH
Expression Host Bacillus subtilis WB800

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide NprB
Signal Peptide Sequence Length = 29 aa
MRNLTKTSLLLAGLCTAAQMVFVTHASAA
Type Sec
Yield 7.68 (U/L)
Performance (Current Yield / Highest Yield) 0.78
Charge 2
Charge (N-Region) 2
Hydrophobicity 0.82
Hydrophobicity Plot
Hydrophobicity (H-region) 1.63
Signal Peptide Original Protein Sequence MRNLTKTSLLLAGLCTAAQMVFVTHASAAESIEYDHTYQTPSYIIEKSPQKPVQNTTQKESLFSYLDKHQTQFKLKGNANSHFRVSKTIKDPKTKQTFFKLTEVYKGIPIYGFEQAVAMKENKQVKSFFGKVHPQIKDVSVTPSISEKKAIHTARRELEASIGKIEYLDGEPKGELYIYPHDGEYDLAYLVRLSTSEPEPGYWHYFIDAKNGKVIESFNAIHEAAGTGIGVSGDEKSFDVTEQNGRFYLADETRGKGINTFDAKNLNETLFTLLSQLIGYTGKEIVSGTSVFNEPAAVDAHANAQAVYDYYSKTFGRDSFDQNGARITSTVHVGKQWNNAAWNGVQMVYGDGDGSKFKPLSGSLDIVAHEITHAVTQYSAGLLYQGEPGALNESISDIMGAMADRDDWEIGEDVYTPGIAGDSLRSLEDPSKQGNPDHYSNRYTGTEDYGGVHINSSIHNKAAYLLAEGGVHHGVQVEGIGREASEQIYYRALTYYVTASTDFSMMKQAAIEAANDLYGEGSKQSASVEKAYEAVGIL
Signal Peptide Original DNA Sequence TTGCGCAACTTGACCAAGACATCTCTATTACTGGCCGGCTTATGCACAGCGGCCCAAATGGTTTTTGTAACACATGCCTCAGCTGAAGAAAGCATCGAATACGACCATACGTATCAAACCCCTTCATACATCATCGAAAAGTCACCGCAGAAGCCGGTACAAAACACAACCCAGAAAGAATCGCTATTTTCCTATCTTGACAAGCATCAAACGCAGTTTAAGCTCAAAGGGAATGCGAACAGCCATTTTCGCGTTTCGAAAACCATAAAGGATCCAAAGACAAAACAAACGTTTTTTAAATTAACGGAGGTTTACAAAGGAATTCCGATTTACGGCTTTGAACAAGCGGTCGCGATGAAGGAAAACAAACAAGTGAAAAGTTTCTTTGGAAAGGTGCATCCGCAAATCAAGGACGTCTCCGTCACACCGTCTATTTCTGAGAAAAAAGCAATACATACAGCAAGGCGTGAGCTCGAGGCTTCCATTGGAAAAATCGAATATCTTGATGGGGAACCAAAAGGCGAATTATATATCTATCCACACGACGGTGAATATGATCTCGCCTACCTTGTGAGACTCTCGACATCTGAACCTGAGCCTGGCTATTGGCATTATTTCATCGATGCCAAAAACGGAAAGGTCATCGAGTCCTTTAATGCCATTCATGAAGCGGCAGGTACAGGAATCGGCGTGTCAGGTGATGAAAAAAGCTTTGACGTCACAGAACAAAATGGGCGCTTTTATTTGGCTGACGAAACAAGGGGAAAAGGGATCAATACATTTGACGCGAAGAACCTGAACGAAACCTTGTTTACGCTTTTGTCTCAACTGATCGGGTATACGGGCAAAGAAATAGTCAGCGGCACGTCCGTATTTAATGAACCTGCGGCTGTAGACGCACACGCAAATGCGCAAGCCGTTTACGATTATTACAGCAAGACATTTGGCCGTGATTCTTTTGATCAAAACGGAGCAAGGATTACGTCTACCGTGCATGTCGGCAAACAATGGAACAATGCTGCGTGGAACGGTGTCCAGATGGTATACGGGGATGGAGACGGTTCGAAATTTAAGCCGCTGTCTGGATCGCTCGACATTGTCGCGCATGAAATCACACACGCAGTCACACAGTATTCCGCCGGTCTTTTATATCAAGGAGAACCCGGTGCATTAAATGAGTCCATTTCTGACATTATGGGCGCGATGGCTGACCGTGATGATTGGGAGATCGGCGAAGATGTCTATACTCCTGGTATTGCAGGAGATTCATTGCGGTCATTGGAGGACCCATCTAAGCAGGGAAATCCAGATCATTACTCGAACCGCTACACAGGAACAGAGGATTATGGCGGAGTCCATATCAATTCGTCCATTCACAATAAAGCAGCTTATCTTCTTGCAGAAGGAGGCGTGCACCACGGTGTACAGGTTGAAGGGATTGGGCGTGAAGCAAGTGAACAAATTTACTATCGGGCTTTAACATATTATGTAACGGCATCTACAGATTTCAGCATGATGAAGCAAGCGGCGATTGAAGCTGCCAATGATTTATACGGTGAAGGCTCGAAGCAATCAGCTTCAGTCGAAAAGGCGTATGAGGCTGTCGGCATTCTATGA
Reference Zhou Y, Xu G, Yang L, et al. Effect of signal Peptide Optimization on Lipase LipS secretion and expression in Bacillus subtilis system[J]. China Biotechnology, 2015, 35(9): 42-49. (in Chinese)
Miscellaneous -