Accession Number |
SPSED0021009 |
Secreted Protein |
Lipase (LipS) |
Uniprot Accession Number |
S4TNY8 |
GenBank Accession Number |
AGF29555.2 |
Source Organism of the Secreted Protein |
Stenotrophomonas maltophilia |
Nucleotide Sequence of the Secreted Protein |
Length = 1581 ntATGCGCAAGGTCCTCCTCCTCCTCATTCTCCTCATTGGCCTCGCCCCTACCGCGTGGGCGGCGATGGCGACGGTCAAGACCACCCATCGCACCATTGCCGGCTGGGATGGAACGCCTCTCGGGGCCTTCGTCATCGAACCGCAGGATGCGGGCGGCGGGCGCTATCCGCTGCTGGTGATGCCCAGCAGCTGGGCGGTGCCCAGCGTGGAGTACGTGGGGGTGGCGCAGTCGCTGGCACAGCGCGGCTATGTGGTGATCAGCTACAGCTCGCGTGGCTTCTGGGAATCCGGCGGCAGCATCGACATCGCCGGGCCCTCCACGGTGGAGGATGTCAGCGCGCTGATCGACTGGGCGCTGGACAACACCCGCGCTGACCCGGACCGGATCGGCGTTTCGGGCATCTCCTACGGTGCGGGCACGAGCCTGCTGGCGGCGGCACGCGATCCGCGCATCAAGGCCGTGGCCGCGCTCAGTGGCTGGGCCGATCTGCAGGCGTCGCTGTACAGCAACGACACCCCGAGCGCGCAGGGCATCGCACTGCTGGTTGCGGCCGGCCTGGTGACCGGACGCCCTGGCGCGGAGCTGGCCACGATCAACCGCAATGTTCTGGCGGGCAACTACCAGGGCGCGGTGGATTCGCTGTTGCCGGTGGCGGCGCAGCGCAGTCCCGCGGCCAGCATCGACGAGATCAACGCCAACCAGCCGGCGGTGTTCCTGGCCAACGCCTTCAACGACAGCCTGTTCCCGCCGGGCCAGCTGGTTGATTTCTTCAACCGGCTGAAAGGCCCGAAGCAGCTGCAGCTGCGCCACGGTGACCACGCGCTCAACGAAGCGCTCGGTGCACTGGGCATTCCCAACGAAGTCTATGACCAGGTGGGTGACTGGTTCGATCACTATCTGAAGGCAGTGGCCAACGGCATCGACCGCCAGCCGGCGGTGCAGCTGAAGTCACAGAAGGGCAGCTGGAGCAGTTACCCGGACTGGCAGGCGACCAGCAAGGGTGCGGTCAGCTACGGCCTGACCGCACCCTCTGGTTTGCTGCTGCCGACCGGCGGCCTGGCCGAGCACGGTGGCGGCACCGGCTGGAACTACCGCATCGGCAGCGGTTTGCTGACTGCGGCCAATTCCGGCGTGGCGATGGCCTCTGGCGCGCTGCAGATGATCAACCTGCCGCCGGGTGCCTACGTACCGTTTGTTGGCCGCAGTGCCGCCGGTGTCTGGCAGGGGCCGATCCAGTGGAGCGCCAAACGCCTGGACGGTGCGCCGGAAGTGCGCCTGACGGTCACGCCCAGCCGTGCCAACACCACGCTGTACGCCTACCTGTACGCCGAGGATGTGCTGGGCAATGGTCAGTTGATCAGCCACAAGCCGTACACCTTGCGCGGTGCCACGCCGGGCCAGGCGAAAACGCTCGACCTGCGGCTGGAAGCGAGCAGCTGGAACCTTCCAGCCGGCAGCCGCCTGACCTTGGTGGTCGATACCGTGGACCTGCGCTATGCGGGCATCAGCCAGCTGGGTGGGGCGGTGACCTTCACCTCGCCAGCCAATGCGCCGTCGGTGCTGAAAGTGCCGCTGCATTGA |
Amino Acid Sequence of the Secreted Protein |
Length = 526 aaMRKVLLLLILLIGLAPTAWAAMATVKTTHRTIAGWDGTPLGAFVIEPQDAGGGRYPLLVMPSSWAVPSVEYVGVAQSLAQRGYVVISYSSRGFWESGGSIDIAGPSTVEDVSALIDWALDNTRADPDRIGVSGISYGAGTSLLAAARDPRIKAVAALSGWADLQASLYSNDTPSAQGIALLVAAGLVTGRPGAELATINRNVLAGNYQGAVDSLLPVAAQRSPAASIDEINANQPAVFLANAFNDSLFPPGQLVDFFNRLKGPKQLQLRHGDHALNEALGALGIPNEVYDQVGDWFDHYLKAVANGIDRQPAVQLKSQKGSWSSYPDWQATSKGAVSYGLTAPSGLLLPTGGLAEHGGGTGWNYRIGSGLLTAANSGVAMASGALQMINLPPGAYVPFVGRSAAGVWQGPIQWSAKRLDGAPEVRLTVTPSRANTTLYAYLYAEDVLGNGQLISHKPYTLRGATPGQAKTLDLRLEASSWNLPAGSRLTLVVDTVDLRYAGISQLGGAVTFTSPANAPSVLKVPLH |
Expression Host |
Bacillus subtilis WB800 |
|
Source Organism of the Signal Peptide |
Bacillus subtilis |
Source Gene of the Signal Peptide |
lipA |
Signal Peptide Sequence |
Length = 32 aaMKFVKRRIIALVTILMLSVTSLFALQPSAKAA |
Type |
Tat |
Yield |
3.31 (U/L) |
Performance (Current Yield / Highest Yield) |
0.34 |
Charge |
5 |
Charge (N-Region) |
4 |
Hydrophobicity |
1.06 |
Hydrophobicity Plot |
|
Hydrophobicity (H-region) |
2.57 |
Signal Peptide Original Protein Sequence |
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN |
Signal Peptide Original DNA Sequence |
ATGAAATTTGTAAAAAGAAGGATCATTGCACTTGTAACAATTTTGATGCTGTCTGTTACATCGCTGTTTGCGTTGCAGCCGTCAGCAAAAGCCGCTGAACACAATCCAGTCGTTATGGTTCACGGTATTGGAGGGGCATCATTCAATTTTGCGGGAATTAAGAGCTATCTCGTATCTCAGGGCTGGTCGCGGGACAAGCTGTATGCAGTTGATTTTTGGGACAAGACAGGCACAAATTATAACAATGGACCGGTATTATCACGATTTGTGCAAAAGGTTTTAGATGAAACGGGTGCGAAAAAAGTGGATATTGTCGCTCACAGCATGGGGGGCGCGAACACACTTTACTACATAAAAAATCTGGACGGCGGAAATAAAGTTGCAAACGTCGTGACGCTTGGCGGCGCGAACCGTTTGACGACAGGCAAGGCGCTTCCGGGAACAGATCCAAATCAAAAGATTTTATACACATCCATTTACAGCAGTGCCGATATGATTGTCATGAATTACTTATCAAGATTAGATGGTGCTAGAAACGTTCAAATCCATGGCGTTGGACACATCGGCCTTCTGTACAGCAGCCAAGTCAACAGCCTGATTAAAGAAGGGCTGAACGGCGGGGGCCAGAATACGAATTAA |
Reference |
Zhou Y, Xu G, Yang L, et al. Effect of signal Peptide Optimization on Lipase LipS secretion and expression in Bacillus subtilis system[J]. China Biotechnology, 2015, 35(9): 42-49. (in Chinese) |
Miscellaneous |
- |