SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0023003

Accession Number SPSED0023003
Secreted Protein α-amylase (AmyS)
Uniprot Accession Number P06279
GenBank Accession Number AAA22235.2
Source Organism of the Secreted Protein Bacillus stearothermophilus
Nucleotide Sequence of the Secreted Protein Length = 1650 nt
GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA
Amino Acid Sequence of the Secreted Protein Length = 549 aa
MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP
Expression Host Bacillus subtilis WS11

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide cwlS
Signal Peptide Sequence Length = 26 aa
MKKKIVAGLAVSAVVGSSMAAAPAEA
Type Sec
Yield 732 (U/ml)
Performance (Current Yield / Highest Yield) 1
Charge 2
Charge (N-Region) 3
Hydrophobicity 0.9
Hydrophobicity Plot
Hydrophobicity (H-region) 2.41
Signal Peptide Original Protein Sequence MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKSTVLYVGQSLKVPESSKKSTTSPSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSSDLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYTVKLGDSLWKIANSLNMTVAELKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIANRLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKIDRMITEAKKYVGVPYRWGGNTPAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSVGDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSNSYWKQRYLGARSYF
Signal Peptide Original DNA Sequence ATGAAAAAGAAGATTGTAGCCGGCTTGGCTGTTTCTGCAGTTGTTGGGTCGTCGATGGCCGCAGCACCCGCGGAAGCAAAAACGATAAAAGTAAAAAGCGGTGACTCTCTTTGGAAACTTTCCCGCCAGTATGATACGACAATATCAGCTCTGAAATCCGAAAACAAATTGAAATCAACGGTGCTTTATGTCGGACAAAGTCTTAAGGTTCCTGAAAGCAGCAAAAAAAGCACGACATCCCCATCTAATTCAAGCAAAACATCAACATACACAGTTGCATATGGTGATTCCCTATGGATGATTGCTAAAAATCATAAAATGAGTGTTTCTGAGCTTAAAAGCTTAAACAGCCTGAGCAGTGACCTGATTCGTCCGGGGCAAAAGCTTAAGATCAAAGGGACAAGCAGTTCAAGCGGATCAAATGGATCAAAGAAAAACAGCGGTTCCAATTCTTCAGGTTCTTCAAAAAGTACATATACGGTCAAGCTTGGTGACTCACTGTGGAAAATCGCAAACAGCTTAAATATGACTGTAGCCGAATTAAAAACCTTAAATGGCTTAACATCAGATACACTTTATCCTAAACAGGTGCTCAAAATAAAAGGAAGCAGTTCACCTAAGAACGGAAACAGCGGTTCCAAAAAGCCGTCAAACTCCAATCCCTCAAAAACAACGACGTACAAGGTAAAAGCAGGCGATTCGCTGTGGAAAATTGCAAATCGGCTCGGAGTCACTGTCCAAAGCATCCGTGATAAAAACAATTTATCATCGGATGTGTTGCAAATCGGGCAGGTTCTGACTATTTCGGGAGCATCAAAATCTAATCCTTCAAATCCGACAAAGCCAACAAAGCCAAAAGATAACTCAGGGTCAAACATTCAAATAGGTTCGAAGATTGACAGAATGATAACGGAAGCGAAAAAATATGTCGGAGTGCCTTACCGATGGGGCGGAAACACGCCTGCCGGTTTTGATTGCAGCGGCTTCATTTATTACCTCATTAATAATGTATCATCTATATCAAGATTGAGTACGGCAGGGTATTGGAATGTCATGCAGAAAGTCAGCCAGCCGTCAGTAGGAGACTTTGTCTTCTTTACCACATATAAATCCGGCCCGTCTCACATGGGGATTTATCTAGGCGGCGGTGATTTTATTCATGCAAGCTCATCGGGCGTCGACATTTCCAATCTCAGCAATTCGTATTGGAAACAGCGGTACTTGGGCGCAAGAAGTTATTTTTAA
Reference Ya D, Su L, Li N, et al. Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection[J]. Microbial Cell Factories, 2019, 18: 69. [PubMed]
Miscellaneous Dual promoters PHpaII and PAmyQ’