SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0026004

Accession Number SPSED0026004
Secreted Protein α-amylase (AmyS)
Uniprot Accession Number P06279
GenBank Accession Number AAA22241.1
Source Organism of the Secreted Protein Geobacillus stearothermophilus
Nucleotide Sequence of the Secreted Protein Length = 1650 nt
GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA
Amino Acid Sequence of the Secreted Protein Length = 549 aa
MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP
Expression Host Bacillus subtilis 1A751

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide ybfo
Signal Peptide Sequence Length = 28 aa
MKRMIVRMTLPLLIVCLAFSSFSASARA
Type Sec
Yield 1175.8 (U/mL)
Performance (Current Yield / Highest Yield) 0.87
Charge 4
Charge (N-Region) 3
Hydrophobicity 1.1
Hydrophobicity Plot
Hydrophobicity (H-region) 1.78
Signal Peptide Original Protein Sequence MKRMIVRMTLPLLIVCLAFSSFSASARAASEEKYWDHWIERHAQPLDASNASNKDLRFLKKVLKGKRIVQLGETTHGAGEINATKVRMIKYLHEELGYDVLAFESGFPDTNASYLNMDQLTPKSTMKNSIYAVWHTEDVVELFDYMKEQKEKGDPLILTGFDIQSMKNSFNVAATQWVKAVDPEKAELLSQSENDFSTLVTDSNTFDEFSQKKEKLVKNYQKLIKFTKTHASELKENLPKEPKAYEMFMHSLQLRIDVMETYMLEEMKEKLEEYPENIEDFSFFMRDRMMAEQFQWVADTLYPKKKIIVWGHNYHLRKQNTKMIKDWVQLNGPNMGDYLPERLKKQTYTIGIYAYSGASLDSSDNKTVKPVTSPPPSGSLEALLKAADRPAVFVDFLHTKNKKGTSWMYTPRTALYWGYMEEQMILKEQYDGVIWLEHITPSVIIK
Signal Peptide Original DNA Sequence TTGAAACGAATGATAGTGAGAATGACCCTGCCGCTGCTGATCGTTTGTCTGGCGTTTTCTTCTTTTTCAGCCTCAGCCCGTGCTGCTTCTGAAGAAAAATATTGGGATCACTGGATTGAACGACATGCACAGCCTCTGGATGCTTCCAATGCTTCAAACAAAGACCTTCGATTTCTAAAAAAGGTGCTGAAGGGAAAACGAATCGTTCAGTTAGGAGAAACCACACACGGTGCTGGCGAAATCAATGCCACAAAGGTCAGAATGATCAAATACCTCCACGAAGAGCTGGGATACGATGTATTGGCCTTTGAATCCGGCTTTCCTGATACAAATGCATCCTACCTTAATATGGACCAATTAACCCCTAAAAGCACGATGAAAAACTCCATTTACGCTGTATGGCACACAGAAGATGTCGTCGAATTGTTTGATTACATGAAAGAGCAAAAAGAGAAAGGCGATCCGCTGATCCTTACCGGTTTTGATATTCAAAGCATGAAGAATTCATTCAATGTAGCTGCGACTCAATGGGTAAAAGCGGTGGACCCTGAGAAAGCAGAGTTGCTCTCCCAATCAGAAAATGATTTTTCAACATTAGTGACAGACAGCAATACGTTTGATGAATTTTCGCAAAAGAAGGAAAAGCTTGTGAAAAACTATCAGAAGCTGATCAAGTTCACTAAAACACATGCTTCAGAGCTAAAAGAAAACCTTCCAAAAGAGCCGAAAGCTTATGAGATGTTTATGCATTCTCTTCAGCTGAGAATTGACGTAATGGAAACATATATGCTGGAAGAAATGAAAGAAAAACTAGAGGAGTATCCGGAGAATATTGAGGATTTTTCATTCTTTATGAGAGACCGCATGATGGCTGAACAATTTCAGTGGGTAGCCGACACCCTTTATCCGAAAAAGAAAATCATTGTCTGGGGCCACAATTACCATCTACGCAAACAGAATACGAAGATGATAAAGGACTGGGTACAGCTAAACGGCCCTAACATGGGTGATTATTTGCCGGAGCGGCTGAAAAAACAAACATATACGATTGGCATTTATGCTTACTCAGGTGCGAGTCTTGATAGCTCCGATAATAAGACTGTTAAGCCGGTTACAAGCCCGCCGCCGTCTGGGAGCCTTGAAGCTTTGCTGAAAGCGGCGGACCGACCGGCTGTTTTTGTTGATTTTCTCCATACAAAAAACAAGAAAGGCACTTCGTGGATGTATACCCCGCGGACTGCGCTCTATTGGGGGTACATGGAAGAGCAGATGATCCTGAAGGAACAATATGACGGTGTCATTTGGCTGGAGCATATCACGCCCTCTGTCATTATCAAGTGA
Reference Jinlan L, Gang F, Huina D, et al. Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis[J]. Industrial Microbiology, 2017, 47(01): 17-23. (In Chinese)
Miscellaneous -