SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0026012
| Accession Number | SPSED0026012 |
| Secreted Protein | α-amylase (AmyS) |
| Uniprot Accession Number | P06279 |
| GenBank Accession Number | AAA22241.1 |
| Source Organism of the Secreted Protein | Geobacillus stearothermophilus | Nucleotide Sequence of the Secreted Protein | Length = 1650 ntGTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA |
| Amino Acid Sequence of the Secreted Protein | Length = 549 aaMLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP |
| Expression Host | Bacillus subtilis 1A751 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | phob |
| Signal Peptide Sequence | Length = 32 aaMKKFPKKLLPIAVLSSIAFSSLASGSVPEASA |
| Type | Sec |
| Yield | 888.4 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.66 |
| Charge | 3 |
| Charge (N-Region) | 4 |
| Hydrophobicity | 0.6 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 1.82 |
| Signal Peptide Original Protein Sequence | MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFDQYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLPKKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDNAIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK |
| Signal Peptide Original DNA Sequence | TTGAAAAAATTCCCGAAGAAATTACTGCCTATCGCGGTTTTATCATCAATTGCGTTCAGCAGCTTAGCCAGCGGCAGTGTGCCTGAAGCCAGCGCCCAGGAAAAGAAAAAGGGGAACCAAGACGAAATTAAAAATGTTATTGTTCTGATTGGTGATGGTATGGGTGTGTCTTATACGTCTGCTTATCGATACTTAAAGGATAATAAAAAGACAAAAGTTGTGGAGCCTACGGCTTTTGATCAGTATCTTGTCGGCCAGCAAACAACTTACCCCGATGATCCTGAACAAAATGTAACGGATTCTGCAGCGGCGGCAACGGCTATGTCTGCGGGAATTAAAACATACAATAATGCCATAGCCGTTGATAACGACGGGTCAGAAGCCAAAACGGTGCTTGAAGCTGCGAAAGAAAAAGGAAAAGCAACAGGACTTGTGGCCACTTCAGAAATTACACACGCCACTCCGGCCTCTTTTGGTTCACACGACCACAGCCGAAAAAATATGAACTCAATTGCGGATGATTATTTTGATGAGATGGTCAACGGCAAGCACAAAATTGATGTCCTTCTAGGCGGAGGAAAGAGCAATTTTGACCGTAAGGACCGTAATTTGATTAAGGAATTCAAAAAGGCGGGGTACAGCTACGTGGATGATCGCAAGGACATGCTGAAAAACAAAGACAGCCAAGTTCTCGGCCTATTCGCTGACGGCGGCCTGCCTAAAAAGATTGACCGTACGAAGGATATTCCATCACTTAAAGATATGACAAACACAGCCATTAAAAAATTAAACAAGGATAAAGACGGATTCTTCCTCATGGTGGAAGGCAGCCAGATTGACTGGGCGGGCCATGACAATGATATCGTTGGCGCCATGAGCGAGATGGAAGACTTCGAACAAGCTTATAAGGCGGCTATTGATTTCGCTAAAAAAGACAAGCACACATTGGTTGTCGCAACGGCTGACCACTCAACCGGCGGATATTCCATCGGTGCGGACGGCATCTACAACTGGTTCAGTGAACCCATTAAAGCCGCAAAGCGCACACCAGACTTTATGGCAGAAAAGATCGCTGACGGCGCGGATGTTGAAAAAACGTTGAAAACATATATCGATCAAAAGAAATTGGCTTTAACAAAAGCCGAAATTCAATCTGTTGAAGAAGCGGCAAAATCGAAGGAAGTACTGGATATCGATAACGCAATCGAGAACATCTTTAATAAACGCTCTCACACTGGATGGACAACAGGCGGACATACCGGTGAAGATGTTCCCGTCTATGCATACGGTCCGTCTAGTGAGACATTTGCCGGCCAAATCGACAATACAGAAATCGCCAAAAATGTTTTCAAGGCTTTACAATACAACATTAAGATTAACGATAAGTAA |
| Reference | Jinlan L, Gang F, Huina D, et al. Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis[J]. Industrial Microbiology, 2017, 47(01): 17-23. (In Chinese) |
| Miscellaneous | - |