SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0026019

Accession Number SPSED0026019
Secreted Protein α-amylase (AmyS)
Uniprot Accession Number P06279
GenBank Accession Number AAA22241.1
Source Organism of the Secreted Protein Geobacillus stearothermophilus
Nucleotide Sequence of the Secreted Protein Length = 1650 nt
GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA
Amino Acid Sequence of the Secreted Protein Length = 549 aa
MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP
Expression Host Bacillus subtilis 1A751

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide wpra
Signal Peptide Sequence Length = 31 aa
MKRRKFSSVVAAVLIFALIFSLFSPGTKAAA
Type Tat
Yield 668.5 (U/mL)
Performance (Current Yield / Highest Yield) 0.5
Charge 5
Charge (N-Region) 4
Hydrophobicity 0.98
Hydrophobicity Plot
Hydrophobicity (H-region) 2.44
Signal Peptide Original Protein Sequence MKRRKFSSVVAAVLIFALIFSLFSPGTKAAAAGAIDQAAALENGKEQTGAMKEPEQVKWYKVTPGATDIQKNSHMALTVKSDSVLNVSVYPSKEKALKDETFEMYRSFTAEDGKSEVIFPYAWSGPYYVKVEYLGEEEPEDGGTAEAAAEAKYTIGYKGTKKQPSDLEEEEACPVEMSVDQKKSGKGILDKLRSIRDEQLSQTAEGKELTSLYYKAAPFIVAKLALNKTARNEIYQDLVTLKPLFDDVSENGASSSYKVTEKDQKAINRLYDKALQSVPSFLKEEIKKQADRLNMKQLQGKTAGAILTENNIAAKSEVQTTKVIFKVKDNKSLSSVHNEMKGFSASAQSKKDISNVKKAKKLFDNLYSFELPKDEKQNGAYTASAKRVKSAAATLSKMSNVEFAEPVQEYKSLANDIQYPYQWPLKNNGENGGVKNADVKYEPANTLLSKRKLNDTLIAVVDTGVDSTLADLKGKVRTDLGHNFVGRNNNAMDDQGHGTHVAGIIAAQSDNGYSMTGLNAKAKIIPVKVLDSAGSGDTEQIALGIKYAADKGAKVINLSLGGGYSRVLEFALKYAADKNVLIAAASGNDGENALSYPASSKYVMSVGATNRMDMTADFSNYGKGLDISAPGSDIPSLVPNGNVTYMSGTSMATPYAAAAAGLLFAQNPKLKRTEVEDMLKKTADDISFESVDGGEEELYDDYGDPIEIPKTPGVDWHSGYGRLNVMKAVSAADLQLKVNKLESTQTAVRGSAKEGTLIEVMNGKKKLGSAKAGKDNAFKVNIATQKQDQVLYLKATKGDAKTSYKVVVVKGKPSGTPKVNAVKTKDTAVKGKANSKAMIRVKNKSKKVIASAKADAKGTFSVKIKKQKAGTVLYVTAVDTDKKESKEAKVVVEK
Signal Peptide Original DNA Sequence ATGAAACGCAGAAAATTCAGCTCGGTTGTGGCGGCAGTGCTTATTTTTGCACTGATTTTCAGCCTTTTTTCTCCGGGAACCAAAGCTGCAGCGGCCGGCGCGATCGATCAGGCGGCTGCTCTGGAAAACGGCAAAGAGCAGACAGGCGCCATGAAGGAGCCGGAACAGGTGAAATGGTACAAAGTGACCCCGGGAGCAACGGATATTCAGAAAAACTCACATATGGCACTGACCGTAAAGAGTGATTCAGTACTGAATGTATCTGTATATCCAAGTAAGGAAAAAGCGCTTAAAGATGAGACGTTTGAGATGTACCGTTCTTTCACAGCGGAGGATGGAAAAAGCGAAGTCATTTTTCCATACGCGTGGAGCGGCCCTTACTATGTAAAAGTTGAATACCTCGGAGAAGAAGAACCAGAGGACGGCGGAACGGCAGAAGCAGCTGCAGAAGCCAAGTATACGATTGGGTATAAAGGCACCAAAAAACAGCCGTCAGATTTAGAAGAGGAAGAAGCTTGTCCGGTTGAAATGAGTGTCGATCAGAAGAAATCAGGAAAAGGCATCCTGGATAAGTTGAGATCGATTCGTGATGAGCAGCTGAGCCAAACAGCAGAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATCTAATGTGAAAAAGGCAAAGAAACTGTTTGACAATCTGTATTCATTTGAACTTCCGAAAGACGAGAAACAGAACGGCGCATATACGGCAAGCGCCAAAAGGGTCAAAAGCGCTGCTGCGACACTATCCAAGATGTCCAATGTAGAGTTTGCGGAACCCGTACAGGAATACAAAAGCTTGGCAAACGATATTCAGTACCCTTATCAATGGCCGCTTAAAAACAACGGTGAAAACGGCGGTGTCAAAAATGCGGATGTGAAATATGAGCCTGCCAACACATTGCTGTCCAAACGCAAGCTTAACGATACACTCATTGCAGTAGTAGACACAGGCGTAGACAGCACGCTTGCCGATTTAAAAGGAAAAGTAAGAACAGATCTCGGACACAATTTTGTCGGACGAAATAACAATGCAATGGATGATCAGGGGCATGGGACGCATGTCGCAGGCATTATTGCAGCCCAAAGCGATAACGGCTATTCAATGACTGGATTGAATGCCAAAGCAAAAATCATCCCTGTAAAAGTGCTTGATTCCGCAGGTTCCGGAGATACTGAACAAATTGCTCTCGGCATCAAATATGCTGCTGACAAAGGAGCAAAGGTGATTAATTTAAGTTTAGGCGGAGGCTACAGCCGCGTGCTTGAATTTGCTTTGAAGTACGCAGCTGACAAAAATGTCTTGATTGCCGCAGCCAGCGGGAATGATGGAGAAAATGCCTTATCTTATCCTGCATCTTCTAAATATGTGATGTCAGTCGGCGCAACGAATCGCATGGATATGACCGCTGATTTCTCTAACTATGGAAAAGGTCTGGACATCTCTGCTCCAGGGTCTGATATCCCGAGCTTAGTGCCGAACGGAAATGTCACGTACATGAGCGGAACGTCTATGGCGACGCCATATGCTGCCGCCGCTGCGGGACTGCTGTTTGCTCAAAATCCTAAGCTAAAAAGAACAGAAGTTGAGGATATGTTGAAAAAGACGGCAGATGATATTTCCTTTGAAAGTGTCGATGGCGGAGAAGAAGAGTTGTATGACGATTATGGCGATCCGATTGAAATTCCGAAGACACCTGGTGTAGACTGGCATTCAGGCTACGGGCGGCTGAATGTCATGAAGGCTGTCAGCGCAGCTGATTTACAGCTTAAGGTCAACAAGCTGGAAAGCACTCAAACAGCTGTCAGAGGAAGCGCGAAGGAAGGCACCCTTATCGAGGTGATGAACGGCAAAAAGAAACTCGGCAGCGCCAAAGCCGGAAAAGACAATGCGTTCAAGGTGAATATCGCGACTCAAAAACAGGATCAAGTACTGTATCTGAAAGCAACAAAAGGCGATGCGAAAACATCGTATAAAGTTGTTGTCGTCAAAGGAAAACCTTCCGGCACACCGAAAGTAAACGCGGTGAAAACGAAGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAA
Reference Jinlan L, Gang F, Huina D, et al. Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis[J]. Industrial Microbiology, 2017, 47(01): 17-23. (In Chinese)
Miscellaneous -